Mouse IgG1 Negative Control antibody

Mouse IgG1 Negative Control:RPE

Product Type
Negative/Isotype Control
Isotype
IgG1
Specificity
Mouse IgG1 Negative Control

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MCA1209PE
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Mouse IgG1 Negative Control antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES

Target Species
Negative Control
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Human T lymphocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat*
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for Mouse IgG1 Negative Control antibody

  1. Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  2. Chen, W. et al. (2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.
    Mol Vis. 15: 619-28.
  3. Safeukui I et al. (2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.
    MBio. 6 (1) pii: e02493-14.
  4. Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
    J Autoimmun. 67: 57-64.
  5. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  6. Stangl, H. et al. (2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
    Sci Rep. 10 (1): 17071.

RRID
AB_322267
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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