CD45 Antibody | OX-1

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CD45 Antibody | OX-1 gallery image 1

Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD45 (MCA43R)

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CD45 Antibody | OX-1 gallery image 2

Staining of rat spleen cells with Mouse anti Rat CD45: RPE (MCA43PE)

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CD45 Antibody | OX-1 gallery image 3

Staining of rat spleen cells with Mouse anti Rat CD45:Alexa Fluor® 488 (MCA43A488)

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CD45 Antibody | OX-1 gallery image 4

Staining of rat spleen cells with Mouse anti Rat CD45:FITC (MCA43FT)

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CD45 Antibody | OX-1 gallery image 5

Figure A. A647 conjugated mouse anti rat CD45 (MCA43A647) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. A647 conjugated mouse anti rat CD45 (MCA43A647) and FITC conjugated mouse anti rat CD45RA (MCA340FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 6

Figure A. RPE conjugated mouse anti rat CD45 (MCA43PE) and FITC conjugated mouse IgG2a isotype control (MCA1210F). Figure B. RPE conjugated mouse anti rat CD45 (MCA43PE) and FITC conjugated mouse anti rat CD26 (MCA924F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 7

Figure A. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse IgG2a isotype control (MCA1210PE). Figure B. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse anti rat CD49d (MCA2872PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 8

Figure A. RPE conjugated mouse anti rat CD49d (MCA2872PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat CD49d (MCA2872PE) and FITC conjugated mouse anti rat CD45 (MCA43F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 9

Figure A. A488 conjugated mouse anti rat CD45 (MCA43A488) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. A488 conjugated mouse anti rat CD45 (MCA43A488) and RPE conjugated mouse anti rat CD86 (MCA2874PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 10

Figure A. PE conjugated mouse anti rat CD86 (MCA2874PE) and A488 conjugated mouse IgG1 isotype control (MCA1209A488). Figure B. PE conjugated mouse anti rat CD86 (MCA2874PE) and A488 conjugated mouse anti rat CD45 (MCA43A488). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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CD45 Antibody | OX-1 gallery image 11

Published customer image:
Mouse anti Rat CD45 antibody, clone OX-1 used for illustration of leukocytes in rat brain by immunohistofluorescence.
Image caption:
Infiltration of monocytes in ATP-injected SNpc.
Sections were obtained 2 d after ATP injection, and double-labeled with Iba-1 and CD45 antibodies. Round Iba-1+ cells were CD45+ (arrows) whereas ramified Iba-1+ cells were CD45- (arrowheads).Scale Bar =50μm (B).

From: Jeong H-K, Ji K-m, Kim B, Kim J, Jou I, Joe E-h (2010)
Inflammatory Responses Are Not Sufficient to Cause Delayed Neuronal Death in ATP-Induced Acute Brain Injury.
PLoS ONE 5(10): e13756.

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  • Mouse anti Rat CD45:FITC
  • Mouse anti Rat CD45:FITC
  • Mouse anti Rat CD45:RPE
  • Mouse anti Rat CD45:RPE-Alexa Fluor® 750
  • Mouse anti Rat CD45:Alexa Fluor® 488
  • Mouse anti Rat CD45
  • Mouse anti Rat CD45
  • Mouse anti Rat CD45:Alexa Fluor® 700
  • Mouse anti Rat CD45:FITC
  • Mouse anti Rat CD45:Alexa Fluor® 647
  • Mouse anti Rat CD45
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-1
  • Isotype
    IgG1
7 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA43FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA43FT
    MCA43GAC *, F, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA43GA
    MCA43RC *, F, IFdatasheet pdfdatasheet pdf0.25 mg
    MCA43R
    MCA43FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA43FB
    MCA43GC *, F, IFdatasheet pdfdatasheet pdf1 mg
    MCA43G
    MCA43PEFdatasheet pdfdatasheet pdf100 Tests
    MCA43PE
    MCA43P750Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA43P750
    MCA43A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA43A488
    MCA43A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA43A700
    MCA43A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA43A647
    MCA43FAFdatasheet pdfdatasheet pdf50 µg
    MCA43FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.

      Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.

      CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

      This product is routinely tested in flow cytometry on rat splenocytes
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
      Purified IgG conjugated to Alexa Fluor® 488-liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 700 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Rat thymocyte membrane glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      release of sequestered calcium ion into cytosol
      negative regulation of protein kinase activity
      focal adhesion
      positive regulation of antigen receptor-mediated signaling pathway
      integral to plasma membrane
      protein kinase regulator activity
      protein dephosphorylation
      T cell receptor signaling pathway
      negative regulation of cytokine-mediated signaling pathway
      negative regulation of T cell mediated cytotoxicity
      response to gamma radiation
      membrane raft
      B cell receptor signaling pathway
      internal side of plasma membrane
      transmembrane receptor protein tyrosine phosphatase activity
      B cell proliferation
      defense response to virus
      protein kinase binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      NeatPack Size: 50 µg, 0.1 mg
      1/5Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      NeatPack Size: 50 µg, 0.1 mg
      1/5Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      NeatPack Size: 50 µg, 0.1 mg
      1/5Pack Size: 0.5 mg
      1/10Pack Size: 50 µg, 0.1 mg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD45 Antibody Formats

    Formats Clone Applications Sizes available
    CD45 Antibody : Purified OX-1 C *, F, IF 0.25 mg | 0.1 mg | 1 mg
    CD45 Antibody : RPE OX-1 F 100 Tests
    CD45 Antibody : RPE-Alexa Fluor® 750 OX-1 F 100 Tests/1ml
    CD45 Antibody : FITC OX-1 F 0.1 mg | 50 µg | 0.5 mg
    CD45 Antibody : Alexa Fluor® 488 OX-1 F 100 Tests/1ml
    CD45 Antibody : Alexa Fluor® 647 OX-1 F 100 Tests/1ml
    CD45 Antibody : Alexa Fluor® 700 OX-1 F 100 Tests/1ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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