CD43 Antibody | W3/13

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CD43 Antibody | W3/13 gallery image 1

Staining of rat splenocytes with Mouse anti Rat CD43: FITC (MCA54FT)

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CD43 Antibody | W3/13 gallery image 2

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. Low power

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CD43 Antibody | W3/13 gallery image 3

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. Med power

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CD43 Antibody | W3/13 gallery image 4

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) followed by peroxidase conjugated Goat anti Mouse IgG1 antibody (STAR132P) for detection. High power

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CD43 Antibody | W3/13 gallery image 5

Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) in red and Mouse anti Rat CD4 (MCA55) in green. Merged image is on the right. Low power

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CD43 Antibody | W3/13 gallery image 6

Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) in red and Mouse anti Rat CD4 (MCA55) in green. Merged image is on the right. Medium power

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CD43 Antibody | W3/13 gallery image 7

Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody (MCA54) in red and Mouse anti Rat CD4 (MCA55) in green. Merged image is on the right. High power

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CD43 Antibody | W3/13 gallery image 8

Published customer image:
Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) used to identify Lymphocytes (a + b) in rat brain following traumatic brain injury using immunohistochemistry. Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar?=?100 μm for (a, c) and d; 50 μm for (b)

From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.

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  • Mouse anti Rat CD43
  • Mouse anti Rat CD43:FITC
  • Mouse anti Rat CD43
  • Mouse anti Rat CD43
  • Mouse anti Rat CD43:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    W3/13
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA54FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA54FT
    MCA54GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA54GA
    MCA54RC, F, P *datasheet pdfdatasheet pdf0.25 mg
    MCA54R
    MCA54GC, F, P *datasheet pdfdatasheet pdf1 mg
    MCA54G
    MCA54PEFdatasheet pdfdatasheet pdf100 Tests
    MCA54PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmebrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)

      Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Rat thymocyte membrane glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      cell surface
      cleavage furrow
      integral to membrane
      thymocyte aggregation
      microvillus
      monocyte activation
      chronic inflammatory response
      protein domain specific binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/25 Pack Size: 0.25 mg
      1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/25 Pack Size: 0.25 mg
      1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/25 Pack Size: 0.25 mg
      1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD43 Antibody Formats

    Formats Clone Applications Sizes available
    CD43 Antibody : RPE W3/13 F 100 Tests
    CD43 Antibody : FITC W3/13 F 0.1 mg
    CD43 Antibody : Purified W3/13 C, F, P * 1 mg | 0.25 mg | 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

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              References

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