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Protocol: ADA Bridging ELISA for Use with Anti-Eculizumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for Use with Anti-Eculizumab Monoclonal Antibodies HCA313, HCA314 and HCA315
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Eculizumab Antibody, catalog number HCA313, HCA314 or HCA315 using eculizumab antibody for capture and detection. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.