Protocol: ADA Bridging ELISA for Use with Anti-Ranibizumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for Use with Anti-Ranibizumab Antibody HCA305 and Anti-Bevacizumab Antibody HCA185
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Ranibizumab Antibody, catalog number HCA305 or Anti-Bevacizumab Antibody HCA185 using ranibizumab antibody for capture and detection. Ranibizumab is a monovalent Fab fragment antibody, and was created from the same parent mouse antibody as bevacizumab. Anti-Bevacizumab Antibody HCA185 also recognizes ranibizumab. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
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