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Protocol: ADA Bridging ELISA for Use with Anti-Evolocumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for Use with Anti-Evolocumab Antibody HCA376, HCA377, or HCA378
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Evolocumab Antibody, catalog number HCA376, HCA377, or HCA378, using evolocumab antibody for capture and detection. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.