Hispec Assay Diluent | N/A

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Hispec Assay Diluent

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
500 ml

50 ml

125 ml

Product Details

Intended Use
HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry.

The effect of HISPEC assay diluent is dependent on assay system and the antibodies used. HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.

HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.

Examples of use:

ELISA: use as a dilution buffer for the specimen and for the detection antibodies
Western blotting: use as dilution buffer for primary and secondary antibodies
Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
Protein arrays: use as dilution buffer for specimen and for the detection antibodies
Product Form
Ready to use assay diluent buffer
Preservative Stabilisers
0.1% Proclin™ 300

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.
Guaranteed until date of expiry. Please see product label.

More Information

Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
For research purposes only

Applications of Hispec Assay Diluent

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
The buffer should be mixed thoroughly immediately before use.
Dilution of the samples:
The standards and samples for ELISA and protein arrays can be diluted with HISPEC assay diluent at 1:2 or higher. Standards and samples should be treated identically.

Dilution of antibodies:
Antibodies can be diluted with HISPEC assay diluent in a user-defined manner, depending on the recommendation of the antibody supplier.

Expected results:
In some cases a reduction in assay signal intensity may be observed when using HISPEC assay diluent. This does not result in a reduction in assay sensitivity due to the accompanying reduction in non-specific binding.

Signal intensity may be increased in some assay systems by use of higher concentrations of antibodies, or by dilution of the HISPEC assay diluent in distilled water (1:2) prior to use. However, these adjustments may also result in some associated increase in non-specific binding.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
ELISA BSA Block BUF032A E 100 ml
ELISA Ultrablock BUF033A E 100 ml
ELISA Synblock BUF034A E 100 ml

Product Specific References

References for Hispec Assay Diluent

  1. Eriksson, O. et al. (2017) Pancreatic imaging using an antibody fragment targeting the zinc transporter type 8: a direct comparison with radio-iodinated Exendin-4.
    Acta Diabetol. Oct 24 [Epub ahead of print].

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