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Protocol: ADA Bridging ELISA for Use with Anti-Denosumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for use with Anti-Denosumab Monoclonal Antibodies HCA280, HCA281 and HCA282
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Denosumab Antibody, HCA280, HCA281 or HCA282 using denosumab antibody for capture and detection. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.