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Progesterone Antibody | 9B4

Progesterone Antibody | 9B4 gallery image 1

Immunoperoxidase staining of paraffin embedded human ovary tissue with Mouse anti Progesterone antibody (7720-1704)

Progesterone Antibody | 9B4 gallery image 2

Immunoperoxidase staining of paraffin embedded human ovary tissue with Mouse anti Progesterone antibody (7720-1704)

  • Progesterone Antibody | 9B4 thumbnail image 1
  • Progesterone Antibody | 9B4 thumbnail image 2
  • Mouse anti Progesterone
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    7720-1704E, P *datasheet pdfdatasheet pdf1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti progesterone antibody, clone 9B4 is specific for progesterone, a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland.

      Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.

      Clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Progesterone conjugated to bovine serum albumin at position 3.
    • Purity
    • Approx. Protein Concentrations
      0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)1/50
      Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Breast carcinoma and normal young ovarian tissue.
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Progesterone Antibody Formats

    Formats Clone Applications Sizes available
    Progesterone Antibody : Purified 9B4 E, P * 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Breast carcinoma and normal young ovarian tissue.
                • Product Images

                • Progesterone Antibody | 9B4 thumbnail image 1
                • Progesterone Antibody | 9B4 thumbnail image 2


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