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CD8 Beta antibody | CA15.4G2

Product Code Applications Pack Size List Price Your Price Qty
MCA1775S
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 2 ml loader
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loader

Mouse anti Dog CD8 beta antibody, clone CA15.4G2 recognises the canine CD8β cell surface antigen, expressed by cytotoxic\suppressor T lymphocytes that interact with MHC Class I expressing targets.

Target Species
Dog
Product Form
Tissue culture supernatant - liquid
Preservative Stabilisers
0.1% sodium azide (NaN3)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1 1/10
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20μl of the suggested working dilution to label 106 cells or 100μl whole blood

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References for CD8 Beta antibody

  1. Moreno, J. et al. (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
    Vet Immunol Immunopathol. 71: 181-95.
  2. Vernau, W. and Moore, P.F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
    Vet Immunol Immunopathol. 69: 145-64.
  3. Sonea, I.M. et al. (2000) Flow cytometric analysis of colonic and small intestinal mucosal lymphocytes obtained by endoscopic biopsy in the healthy dog.
    Vet Immunol Immunopathol. 77: 103-19.
  4. Pumarola, M. et al. (2004) Canine inflammatory myopathy: analysis of cellular infiltrates.
    Muscle Nerve. 29: 782-9.
  5. Wilkerson, M.J. et al. (2005) Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules.
    Vet Immunol Immunopathol. 106: 179-96.
  6. Gauthier, M.J. et al. (2005) The immunophenotype of peripheral blood lymphocytes in clinically healthy dogs and dogs with lymphoma in remission.
    J Vet Intern Med. 19: 193-9.
  7. Kisseberth, W.C. et al. (2007) A novel canine lymphoma cell line: a translational and comparative model for lymphoma research.
    Leuk Res. 31: 1709-20.
  8. Luckschander, N. et al. (2009) Phenotyping, functional characterization, and developmental changes in canine intestinal intraepithelial lymphocytes.
    Vet Res. 40: 58.
  9. View The Latest Product References
  10. Izci C et al. (2015) Clinical and light microscopic studies of the conjunctival tissues of dogs with bilateral keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine.
    Biotech Histochem. 90 (3): 223-30.

Further Reading

  1. Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T lymphocyte subsets and high density expression of CD4 by canine neutrophils.
    Tissue Antigens 40: 75-85.

Flow Cytometry

RRID
AB_1220541

MCA1775S

150185

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