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CD46 antibody | 6D8/8

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MCA2310GA
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SDS Safety Datasheet SDS
F IP WB 0.1 mg loader
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loader

Mouse anti Pig CD46, clone 6D8/8 recognizes the porcine CD46 cell surface antigen, a ~50-60 kDa glycoprotein also known as membrane cofactor protein (MCP).

Porcine CD46 is expressed on a wide range of cells including leucocytes, platelets and erythrocytes. CD46 serves as an inhibitor of complement activation on host cells.

Mouse anti Pig CD46, clone 6D8/8 induces homotypic cell aggregation, (Bullido et al. 2000). .

Mouse anti pig CD46, clone 6D8/8 detects a series of closely apposed bands of approximately 50-60 kDa in pig erythrocyte lysates (Perez De La Lastra et al. 1999).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to 1x106 cells in 100μl

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References for CD46 antibody

  1. Perez de la Lastra, J.M. et al. (1999) Epitope mapping of 10 monoclonal antibodies against the pig analogue of human membrane cofactor protein (MCP).
    Immunology 96: 663-670.
  2. Bullido, R. et al. (2000) Induction of aggregation in porcine lymphoid cells by antibodies to CD46.
    Vet Immunol Immmunopathol. 73: 73-81.
  3. Dräger, C. et al. (2015) Porcine complement regulatory protein CD46 and heparan sulfates are the major factors for classical swine fever virus attachment in vitro.
    Arch Virol. 160: 739-46.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
Membrane CO-FACTOR Protein
UniProt
O02839
Entrez Gene
CD46
GO Terms
GO:0002079 inner acrosomal membrane
GO:0016021 integral to membrane
GO:0006958 complement activation, classical pathway
GO:0007338 single fertilization
GO:0045087 innate immune response

MCA2310GA

1601 161373 167515

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