Respiratory Syncytial Virus Nucleoprotein antibody | RSV3132 (B023)
Mouse anti Respiratory Syncytial Virus nucleoprotein antibody, clone RSV3132 (MCA491G) used to validate an anti RSN Nprotein ELISA
Image caption:
Validation of anti-nucleoprotein (N) ELISA. Different amounts of purified his-tagged N protein (Sino Biological) were incubated on nickel-coated plates overnight. Plates were blocked, washed, and then incubated with the following primary Abs: Anti-N monoclonal Ab (AbD serotec), pooled sera from mice (n=5) twice infected with wt RSV, or pooled sera from mock-infected mice (n=5). After primary Abs, plates were processed for standard ELISA. Error bars represent the standard deviation of the mean of triplicate samples (technical replicates of pooled sera).
From: Terhüja, M.; Siddappa, M.; Lamichhane, P.; Meshram, C.D.; Snider, T.A.; Ritchey, J.W.; Oomens, A.G.P.
Intranasal Vaccination with a Respiratory-Syncytial-Virus-Based Virus-like Particle Displaying the G Protein Conserved Region Induces Severe Weight Loss and Pathology upon Challenge with Wildtype Respiratory Syncytial Virus.
Viruses 2024, 16, 843.
doi: 10.3390/v16060843.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Respiratory Syncytial Virus Nucleoprotein
- Product Type
- Monoclonal Antibody
- Clone
- RSV3132 (B023)
- Isotype
- IgG1
- Specificity
- Respiratory Syncytial Virus Nucleoprotein
Antibody Quality and Performance Guarantee
| Mouse anti Respiratory Syncytial Virus Nucleoprotein antibody, clone RSV3132 (B023) recognizes an epitope within the 42kD RSV nucleoprotein. Mouse anti Respiratory Syncytial Virus Nucleoprotein antibody, clone RSV3132 (B023) can be used in immunofluorescence assays in conjunction with clone RSV3216. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Bovine RSV strains: 127, SNK and 9007. Human RSV strains: A/2, Long, Randall and 8/60.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from BALB/c mice were fused with cells of the NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/100 |
- 1Suitable for use as detection reagent with MCA490 as capture reagent in sandwich ELISA (See Adams et al. 2010 for details).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their system using appropriate negative/positive controls.
References for Respiratory Syncytial Virus Nucleoprotein antibody
-
Adams, O. et al. (2010) Palivizumab-resistant human respiratory syncytial virus infection in infancy.
Clin Infect Dis. 51 (2): 185-8. -
Bajimaya, S. et al. (2017) Cholesterol is required for stability and infectivity of influenza A and respiratory syncytial viruses.
Virology. 510: 234-41. -
Terhüja, M. et al. (2024) Intranasal Vaccination with a Respiratory-Syncytial-Virus-Based Virus-like Particle Displaying the G Protein Conserved Region Induces Severe Weight Loss and Pathology upon Challenge with Wildtype Respiratory Syncytial Virus
Viruses. 16 (6): 843.
- RRID
- AB_324337
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