Influenza B Nucleoprotein antibody | B114
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Mouse anti Influenza B Nucleoprotein
- Product Type
- Monoclonal Antibody
- Clone
- B114
- Isotype
- IgG2b
- Specificity
- Influenza B Nucleoprotein
Antibody Quality and 
Unrivaled 
| Mouse anti Influenza B Nucleoprotein antibody, clone B114 recognises a species specific conserved epitope within the nucleoprotein of influenza B virus. The influenza viruses, classified as type A, B and C, are members of the Orthomyxoviridae family which differ in their epidemiology and host ranges and lack serological cross-reactivity of their internal components, especially their matrix proteins and nucleoprotein. Influenza B virus is a slow-mutating single stranded RNA virus subject to antigenic drift which, although enough to prevent lasting immunity, prevents influenza B from causing pandemics. Mouse anti Influenza B Nucleoprotein antibody, clone B114 can be used in influenza B IFA typing in conjunction with MCA403 (clone B017). |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Influenza B/Lee/40 and B/Singapore/-222/79 viruses.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Londo, D.R. et al. (1983) Complete nucleotide sequence of the nucleoprotein gene of influenza B virus.
J Virol. 47 (3): 642-8.
- RRID
- AB_915341
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