Influenza A H1N1 Hemagglutinin antibody | C102 (IV.C102)
Mouse anti influenza A H1N1 hemagglutinin antibody, clone C102 (5315-2907) used to measure hemagglutinin levels using Raman spectral scattering.
Image caption:
SERS spectra of the half sandwich assay with three different kinds of influenza H1N1-VLPs (red), H3N2-VLPs (green), and H5N1-VLPs (blue) against a series of antibodies. The Raman signal at 1290 cm-1 from the NC membrane is consistent and used as an internal standard here. Bar graphs show the intensities of the SERS peaks at 1340 cm-1 for different subtypes of influenza VLP.
From: Lin YJ, Wu CY, Li T, Hsiao PW, Chang DK (2014)
A Rapid and Sensitive Early Diagnosis of Influenza Virus Subtype via Surface Enhanced Raman Scattering.
J Biosens Bioelectron 5:150.
Mouse anti influenza A H1N1 hemagglutinin antibody, clone C102 (5315-2907) used for the evaluation of viral load in MDCK cells by flow cytometry.
Image caption
Binding of porcine NKp46 Ig fusion protein to of influenza A virus infected MDCK cells analysed by flow cytometry. Cells were gated according to forward/side scatter characteristics. (A) Histograms show binding of different concentrations of NKp46-Ig to infected cells. Percentages of positively stained cells are indicated. (B) Infected as well as non-infected MDCK were stained with anti-H1 mAbs. Corresponding isotype-matched irrelevant mAbs and secondary antibodies only served as controls. (C) Double staining for H1 and the highest concentration of NKp46-Ig are shown on influenza infected cells. Results are representative of four independent experiments.
From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus. PLoS ONE 9(6): e100619.
Mouse anti Influenza A H1N1 Hemagglutinin
- Product Type
- Monoclonal Antibody
- Clone
- C102 (IV.C102)
- Isotype
- IgG1
- Specificity
- Influenza A H1N1 Hemagglutinin
Antibody Quality and Performance Guarantee
| Mouse anti Influenza A heamagglutinin antibody, clone C102 recognizes the haemagglutinin of the Influenza A H1N1 serotype and does not react with H2N2, H3N2, Influenza B, RSV, Parainfluenza I, II or III, Adenovirus, mumps or measle antigens. Mouse anti Influenza A heamagglutinin antibody, clone C102 has been used sucessfully for the detection of H1 influenza virus in a Surface Enhanced Raman Scattering biosensor assay demonstrating lack of reactivity with either H3 or H5 influenza A strains (Lin et al. 2014). |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- InfluenzaA strain: A/USSR/90/77.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
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| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Influenza A H1N1 Hemagglutinin antibody
-
Lin, Y.J. et al. (2014) A Rapid and Sensitive Early Diagnosis of Influenza Virus Subtype via Surface Enhanced Raman Scattering.
J Biosens Bioelectron 5: 150. -
Forberg, H. et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
PLoS One. 9:e100619. -
Canelle, Q. et al. (2016) Evaluation of potential immunogenicity differences between Pandemrix™ and Arepanrix™.
Hum Vaccin Immunother. 12 (9): 2289-98.
- RRID
- AB_620586
- UniProt
- P03453
- GO Terms
- GO:0016021 integral to membrane
- GO:0019031 viral envelope
- GO:0019064 viral envelope fusion with host membrane
- GO:0020002 host cell plasma membrane
- GO:0046789 host cell surface receptor binding
- GO:0055036 virion membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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