Feline Immunodeficiency Virus p24 antibody | NM3-6A9

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Mouse anti Feline Immunodeficiency Virus p24

Product Type
Monoclonal Antibody
Clone
NM3-6A9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4413-5107 E F WB 0.2 mg
Mouse anti Feline immunodeficiency virus p24 antibody, clone NM3-6A9 recognizes feline immunodeficiency virus (FIV) p24 gag capsid protein, derived from the p50 GAG polyprotein. The p24 capsid protein forms the conical viral core encapsulating the genomic RNA-nucleocapsid complex and is involved in viral packaging and subsequent release from infected cells. FIV is a lentivirus infecting many domestic and wild cat species, resulting in a progressive immunodeficiency disease bearing a number of parallels with HIV-1 infection in man (Sparger et al. 1989).

Mouse anti Feline immunodeficiency virus p24 antibody, clone NM3-6A9 does not recognise Feline Leukemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus.

Mouse anti feline immunodeficiency virus p24, clone NM3-6A9 recognizes a different epitope to clone PAK3-2C1.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
Immunogen
Native, extracts
Approx. Protein Concentrations
1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Feline Immunodeficiency Virus p24 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 µg/well
Flow Cytometry 1/50 1/100
Western Blotting 1/25 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
4413-5107 detects a major band of approximately 24kDa

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Feline Immunodeficiency Virus p24 gag MCA2278 0.25 mg C* E F* IF WB

Product Specific References

Further Reading

  1. Kenyon, J.C. and Lever, A.M. (2011) The molecular biology of feline immunodeficiency virus (FIV).
    Viruses. 3: 2192-213.
  2. Luttge, B.G. and Freed, E.O. (2010) FIV Gag: virus assembly and host-cell interactions.
    Vet Immunol Immunopathol. 134: 3-13.
  3. Willett, B.J. et al. (1997) FIV infection of the domestic cat: an animal model for AIDS.
    Immunol Today. 18: 182-9.