Feline Herpes Virus Type 1 antibody | FHV7-7C
Mouse anti Feline herpesvirus type 1 antibody, clone FHV7-7C (MCA2490) used to detect virus infected cells in feline corneal explants by immunofluorescence.
Image caption:
The feline whole corneal explant model is susceptible for feline herpesvirus type 1 infection.
(c) Mock- or FHV-1-infected corneal cross-sections were stained with hematoxilin and eosin (H&E), or with monoclonal anti-FHV-1 antibodies followed by Alexa Fluor 488-conjugated secondary antibodies. Nuclei were stained with DAPI. Bars, 100 μm.
From: Pennington MR, Fort MW, Ledbetter EC, Van de Walle GR.
A novel corneal explant model system to evaluate antiviral drugs against feline herpesvirus type 1 (FHV-1).
J Gen Virol. 2016 Jun;97(6):1414-1425.
doi: 10.1099/jgv.0.000451
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline herpesvirus type 1 antibody, clone FHV7-7C (MCA2490) used to detect virus infected cells in feline corneal explants by immunofluorescence.
Image caption:
Incubation of FHV-1-infected corneas with cidofovir or acyclovir reduces FHV-1 viral load and epithelial damage (n=3).
(c) Cidofovir and acyclovir significantly reduce FHV-1 replication in feline corneas. Paired corneas from the same cat were both infected with 3×105 PFU FHV-1 and were either left untreated or treated every 12 h with ~ 2× the EC50 of cidofovir (16.2 μM) or acyclovir (181 μM) and produced infectious virus in the supernatants was assessed by standard plaque assays every 12 h (c); dotted lines represent FHV-1-infected, untreated matched corneas set to 100 %. (d) Cidofovir and acyclovir reduce FHV-1 viral antigen-positive staining in feline corneas. Corneal cross sections were stained with monoclonal anti-FHV-1 antibodies followed by Alexa Fluor 488-conjugated secondary antibodies, and nuclei were stained with DAPI. Bars, 100 μm.
From: Pennington MR, Fort MW, Ledbetter EC, Van de Walle GR.
A novel corneal explant model system to evaluate antiviral drugs against feline herpesvirus type 1 (FHV-1).
J Gen Virol. 2016 Jun;97(6):1414-1425.
doi: 10.1099/jgv.0.000451
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline herpesvirus type 1 antibody, clone FHV7-7C (MCA2490) used to detect virus infected cells in feline corneal explants by immunofluorescence.
Image caption:
Incubation of FHV1-infected corneas with raltegravir reduces FHV-1 viral load and epithelial damage (n=3). Paired corneas from the same cat were both infected with 3×105 PFU FHV-1 and were either left untreated or treated every 24 h with ~2× the EC50 of raltegravir (500 μM). (a) Raltegravir significantly reduces FHV-1 replication in feline corneas. Viral load in the cornea was assessed by qPCR after 48 h, and produced infectious virus in the supernatants was assessed by standard plaque assays every 24 h. Dotted line represents the viral load in supernatants collected from FHV-1 infected, untreated matched corneas, set to 100 %. (b) Corneal cross-sections were stained with monoclonal anti-FHV-1 antibodies followed by Alexa Fluor 488-conjugated secondary antibodies, and nuclei were stained with DAPI. Bars, 100 μm.
From: Pennington MR, Fort MW, Ledbetter EC, Van de Walle GR.
A novel corneal explant model system to evaluate antiviral drugs against feline herpesvirus type 1 (FHV-1).
J Gen Virol. 2016 Jun;97(6):1414-1425.
doi: 10.1099/jgv.0.000451
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Feline Herpes Virus Type 1
- Product Type
- Monoclonal Antibody
- Clone
- FHV7-7C
- Isotype
- IgG2a
- Specificity
- Feline Herpes Virus Type 1
Antibody Quality and Performance Guarantee
| Mouse anti Feline herpesvirus type 1 antibody, clone FHV7-7C recognizes feline Herpesvirus type 1 (FHV-1), of the genus Herpesviridae. FHV-1 is worldwide, and the most clinically significant pathogen for feline respiratory infections, being the causative agent of feline viral rhinotracheitis (FVR). Clone FHV7-7C specifically reacts with three different cultured FHV isolates, but does not cross react with feline Calcivirus (FCV), feline Immunodeficiency virus (FIV) or feline Leukaemia virus (FeLV). |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
||
| Western Blotting 1 | |
- 1Clone FHV7-7C recognizes feline herpes virus under reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Feline Herpes Virus Type 1 antibody
-
Lee, M. et al. (2010) Immunohistological evaluation of feline herpesvirus-1 infection in feline eosinophilic dermatoses or stomatitis.
J Feline Med Surg. 12 (2): 72-9. -
Verweij, M.C. et al. (2011) The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus Varicellovirus.
J Virol. 85 (5): 2351-63. -
Synowiec, A. et al. (2023) Feline herpesvirus 1 (FHV-1) enters the cell by receptor-mediated endocytosis.
J Virol. 97 (8): e0068123. -
Pennington, M.R. et al. (2016) A novel corneal explant model system to evaluate antiviral drugs against feline herpesvirus type 1 (FHV-1).
J Gen Virol. 97 (6): 1414-1425. -
Pennington, M.R. et al. (2018) Transcriptome profiling of alphaherpesvirus-infected cells treated with the HIV-integrase inhibitor raltegravir reveals profound and specific alterations in host transcription.
J Gen Virol. 99 (8): 1115-1128. -
Pennington, M.R. & Van, G.W.D. (2017) Electric Cell-Substrate Impedance Sensing To Monitor Viral Growth and Study Cellular Responses to Infection with Alphaherpesviruses in Real Time.
mSphere. 2 (2)Apr 05 [Epub ahead of print].
Further Reading
-
Maeda, K. et al. (1998) Properties and functions of feline herpesvirus type 1 glycoproteins.
J Vet Med Sci. 60 (8): 881-8.
- RRID
- AB_609663
MCA2490
148859
152318
156725
180608
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