Canine Distemper Virus antibody | DV2-12

Mouse anti Canine Distemper Virus

Product Type
Monoclonal Antibody
Clone
DV2-12
Isotype
IgG2b
Specificity
Canine Distemper Virus

Product Code Applications Pack Size List Price Your Price Qty
MCA1893
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P * WB 0.25 mg loader
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loader
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Mouse anti canine distemper virus antibody, clone DV2-12 recognizes canine distemper virus nucleoprotein. In western blots the antibody recognizes bands of ~76 kDa, 68 kDa, 54 kDa and 32 kDa under reducing conditions.

Mouse anti canine distemper virus antibody, clone DV2-12 is useful for the detection of CDV in immunofluorescence procedures, in which the staining seen has a cytoplasmic pattern in infected cells. It is also effective in paraffin embedded material.

Mouse anti canine distemper virus antibody, clone DV2-12 is anti morbillivirus specific and reacts with phocine distemper virus, raccoon distemper virus and human measles virus. It does not cross react with CAV2, CCV, CPI or CPV.

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Canine Distemper Virus antibody

  1. Guvenc, T. et al. (2008) Immunohistochemical distribution of alpha B-crystallin in the cerebellum of dogs infected with canine distemper virus.
    Acta Vet Hung. 56: 117-23.
  2. Headley, S.A. et al. (2009) Diagnostic exercise: Tyzzer's disease, distemper, and coccidiosis in a pup. .
    Vet Pathol. 46: 151-4.
  3. Headley, S.A. et al. (2009) Molecular detection of Canine distemper virus and the immunohistochemical characterization of the neurologic lesions in naturally occurring old dog encephalitis.
    J Vet Diagn Invest. 21: 588-97.
  4. Bathen-Noethen, A. et al. (2008) Magnetic resonance imaging findings in acute canine distemper virus infection.
    J Small Anim Pract. 49: 460-7.
  5. Kyöstilä, K. et al. (2012) A SEL1L mutation links a canine progressive early-onset cerebellar ataxia to the endoplasmic reticulum-associated protein degradation (ERAD) machinery.
    PLoS Genet. 8 (6): e1002759.
  6. Headley, S.A. and Sukura, A. (2009) Naturally occurring systemic canine distemper virus infection in a pup
    Braz J Vet Pathol 2: 95-101.
  7. Kubo, M. et al. (2010) Meningoencephalitis associated with Sarcocystis spp. in a free-living Japanese raccoon dog (Nyctereutes procyonoides viverrinus).
    J Comp Pathol. 143: 185-9.
  8. Sánchez-Migallón Guzmán, D. et al. (2008) Aleutian disease serology, protein electrophoresis, and pathology of the European mink (Mustela lutreola) from Navarra, Spain.
    J Zoo Wildl Med. 39: 305-13.
  9. View The Latest Product References
  10. Szentiks, C.A. et al. (2013) Polar bear encephalitis: establishment of a comprehensive next-generation pathogen analysis pipeline for captive and free-living wildlife.
    J Comp Pathol. 2014 May;150(4):474-88.
  11. Rentería-Solís, Z. et al. (2014) Canine distemper outbreak in raccoons suggests pathogen interspecies transmission amongst alien and native carnivores in urban areas from Germany.
    Vet Microbiol. 174 (1-2): 50-9.
  12. Woo, G.H. et al. (2010) Canine distemper virus infection in fennec fox (Vulpes zerda).
    J Vet Med Sci. 72 (8): 1075-9.
  13. Spitzbarth, I. et al. (2010) Immunohistochemical characterization of inflammatory and glial responses in a case of necrotizing leucoencephalitis in a French bulldog.
    J Comp Pathol. 142 (2-3): 235-41.
  14. Liang, C.T. et al. (2007) A non-biotin polymerized horseradish-peroxidase method for the immunohistochemical diagnosis of canine distemper.
    J Comp Pathol. 136 (1): 57-64.
  15. Bak, E.J. & , Woo, G.H. (2016) Canine Distemper in Albino Ferrets (Mustela putorius furo).
    J. Comp Path. 154 (1): 121.

RRID
AB_323083