Canine Distemper Virus antibody | DV2-12
Mouse anti canine distemper virus antibody, clone DV2-12 (MCA1893) used to label CDV in canine brain tissue by immunohistochemistry
Image caption:
Canine distemper virus infection in domestic dogs.CDV stainings at three progessive stages (normal, sub-acute and chronic), CDV immunoreactive particle deposition is shown in each stage. Figure S1. (g) Demyelination is not detected. Dog of Control group. (g) Immunohistochemistry against Canine Distemper Virus (CDV) ribonucleoproteins. Scale=500μm. (h) Demyelination is apparent. Dog of Subacute (h) Immunohistochemistry against Canine Distemper Virus (CDV) ribonucleoproteins. Scale=500μm. (i) Demyelination is severe. Dog of Chronic group. (i) Immunohistochemistry against Canine Distemper Virus (CDV) ribonucleoproteins. Scale=500μm
From: Iribarnegaray, V.; Godiño, G.; Larrañaga, C.; Yamasaki, K.; Verdes, J. M.; Puentes, R.
Droplet Digital Pcr Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection.
Preprints 2024, 2024091390.
doi: 10.20944/preprints202409.1390.v1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Canine Distemper Virus
- Product Type
- Monoclonal Antibody
- Clone
- DV2-12
- Isotype
- IgG2b
- Specificity
- Canine Distemper Virus
Antibody Quality and Performance Guarantee
| Mouse anti canine distemper virus antibody, clone DV2-12 recognizes canine distemper virus nucleoprotein. In western blots the antibody recognizes bands of ~76 kDa, 68 kDa, 54 kDa and 32 kDa under reducing conditions. Mouse anti canine distemper virus antibody, clone DV2-12 is useful for the detection of CDV in immunofluorescence procedures, in which the staining seen has a cytoplasmic pattern in infected cells. It is also effective in paraffin embedded material. Mouse anti canine distemper virus antibody, clone DV2-12 is anti morbillivirus specific and reacts with phocine distemper virus, raccoon distemper virus and human measles virus. It does not cross react with CAV2, CCV, CPI or CPV. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
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| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Canine Distemper Virus antibody
-
Liang, C.T. et al. (2007) A non-biotin polymerized horseradish-peroxidase method for the immunohistochemical diagnosis of canine distemper.
J Comp Pathol. 136 (1): 57-64. -
Sánchez-Migallón Guzmán, D. et al. (2008) Aleutian disease serology, protein electrophoresis, and pathology of the European mink (Mustela lutreola) from Navarra, Spain.
J Zoo Wildl Med. 39: 305-13. -
Bathen-Noethen, A. et al. (2008) Magnetic resonance imaging findings in acute canine distemper virus infection.
J Small Anim Pract. 49: 460-7. -
Guvenc, T. et al. (2008) Immunohistochemical distribution of alpha B-crystallin in the cerebellum of dogs infected with canine distemper virus.
Acta Vet Hung. 56: 117-23. -
Headley, S.A. and Sukura, A. (2009) Naturally occurring systemic canine distemper virus infection in a pup
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Headley, S.A. et al. (2009) Molecular detection of Canine distemper virus and the immunohistochemical characterization of the neurologic lesions in naturally occurring old dog encephalitis.
J Vet Diagn Invest. 21: 588-97. -
Headley, S.A. et al. (2009) Diagnostic exercise: Tyzzer's disease, distemper, and coccidiosis in a pup.
Vet Pathol. 46: 151-4. -
Spitzbarth, I. et al. (2010) Immunohistochemical characterization of inflammatory and glial responses in a case of necrotizing leucoencephalitis in a French bulldog.
J Comp Pathol. 142 (2-3): 235-41.
View The Latest Product References
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Woo, G.H. et al. (2010) Canine distemper virus infection in fennec fox (Vulpes zerda).
J Vet Med Sci. 72 (8): 1075-9. -
Kubo, M. et al. (2010) Meningoencephalitis associated with Sarcocystis spp. in a free-living Japanese raccoon dog (Nyctereutes procyonoides viverrinus).
J Comp Pathol. 143: 185-9. -
Kyöstilä, K. et al. (2012) A SEL1L mutation links a canine progressive early-onset cerebellar ataxia to the endoplasmic reticulum-associated protein degradation (ERAD) machinery.
PLoS Genet. 8 (6): e1002759. -
Szentiks, C.A. et al. (2013) Polar bear encephalitis: establishment of a comprehensive next-generation pathogen analysis pipeline for captive and free-living wildlife.
J Comp Pathol. 2014 May;150(4):474-88. -
Rentería-Solís, Z. et al. (2014) Canine distemper outbreak in raccoons suggests pathogen interspecies transmission amongst alien and native carnivores in urban areas from Germany.
Vet Microbiol. 174 (1-2): 50-9. -
Bak, E.J. & , Woo, G.H. (2016) Canine Distemper in Albino Ferrets (Mustela putorius furo).
J. Comp Path. 154 (1): 121. -
Çomakli, S. et al. (2020) Canine distemper virus induces downregulation of GABAA, GABAB, and GAT1 expression in brain tissue of dogs.
Arch Virol. 165 (6): 1321-31. -
Iribarnegaray, V. et al. (2024) Droplet Digital PCR Enhances Sensitivity and Accuracy in Canine Distemper Virus Detection
Preprints 18 Sept [Epub ahead of print]. -
Garcia, P.A.T. et al. (2022) Distribution of canine distemper virus and nectin-4 in raccoon (Procyon lotor) skin.
Vet Pathol. 59 (5): 782-786.
- RRID
- AB_323083
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