Canine Distemper Virus antibody | DV2-12

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Mouse anti Canine Distemper Virus

Product Type
Monoclonal Antibody
Clone
DV2-12
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1893
Datasheet
C E P* WB
MSDS
0.25 mg loader

Mouse anti canine distemper virus antibody, clone DV2-12 recognizes canine distemper virus nucleoprotein. In western blots the antibody recognises bands of ~76 kDa, 68 kDa, 54 kDa and 32 kDa under reducing conditions.

Mouse anti canine distemper virus antibody, clone DV2-12 is useful for the detection of CDV in immunofluorescence procedures, in which the staining seen has a cytoplasmic pattern in infected cells. It is also effective in paraffin embedded material.

Mouse anti canine distemper virus antibody, clone DV2-12 is anti morbillivirus specific and reacts with phocine distemper virus, racoon distemper virus and human measles virus. It does not cross react with CAV2, CCV, CPI or CPV.

Product Details

Target Species
Viral
Species Cross-Reactivity
Target SpeciesCross Reactivity
Raccoon dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Canine Distemper Virus antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Serotec target unmasking fluid (STUF) product code BUF025A/B/C is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Canine Distemper Virus antibody

  1. Guvenc, T. et al. (2008) Immunohistochemical distribution of alpha B-crystallin in the cerebellum of dogs infected with canine distemper virus.
    Acta Vet Hung. 56: 117-23.
  2. Headley, S.A. et al. (2009) Diagnostic exercise: Tyzzer's disease, distemper, and coccidiosis in a pup. .
    Vet Pathol. 46: 151-4.
  3. Headley, S.A. et al. (2009) Molecular detection of Canine distemper virus and the immunohistochemical characterization of the neurologic lesions in naturally occurring old dog encephalitis.
    J Vet Diagn Invest. 21: 588-97.
  4. Bathen-Noethen, A. et al. (2008) Magnetic resonance imaging findings in acute canine distemper virus infection.
    J Small Anim Pract. 49: 460-7.
  5. Kyöstilä, K. et al. (2012) A SEL1L mutation links a canine progressive early-onset cerebellar ataxia to the endoplasmic reticulum-associated protein degradation (ERAD) machinery.
    PLoS Genet. 8 (6): e1002759.
  6. Headley, S.A. and Sukura, A. (2009) Naturally occurring systemic canine distemper virus infection in a pup
    Braz J Vet Pathol 2: 95-101.
  7. Kubo, M. et al. (2010) Meningoencephalitis associated with Sarcocystis spp. in a free-living Japanese raccoon dog (Nyctereutes procyonoides viverrinus).
    J Comp Pathol. 143: 185-9.
  8. Sánchez-Migallón Guzmán, D. et al. (2008) Aleutian disease serology, protein electrophoresis, and pathology of the European mink (Mustela lutreola) from Navarra, Spain.
    J Zoo Wildl Med. 39: 305-13.
  9. Szentiks, C.A. et al. (2013) Polar bear encephalitis: establishment of a comprehensive next-generation pathogen analysis pipeline for captive and free-living wildlife.
    J Comp Pathol. 2014 May;150(4):474-88.
  10. Rentería-Solís, Z. et al. (2014) Canine distemper outbreak in raccoons suggests pathogen interspecies transmission amongst alien and native carnivores in urban areas from Germany.
    Vet Microbiol. 174 (1-2): 50-9.
  11. Woo, G.H. et al. (2010) Canine distemper virus infection in fennec fox (Vulpes zerda).
    J Vet Med Sci. 72 (8): 1075-9.
  12. Spitzbarth, I. et al. (2010) Immunohistochemical characterization of inflammatory and glial responses in a case of necrotizing leucoencephalitis in a French bulldog.
    J Comp Pathol. 142 (2-3): 235-41.
  13. Liang, C.T. et al. (2007) A non-biotin polymerized horseradish-peroxidase method for the immunohistochemical diagnosis of canine distemper.
    J Comp Pathol. 136 (1): 57-64.
  14. Bak, W.E. and Woo, G.H. (2016) Canine Distemper in Albino Ferrets (Mustela putorius furo)
    J comp Pathol. 154: 121