Antigen Retrieval Buffer, pH8.0

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  • Antigen Retrieval Buffer, pH8.0
  • Antigen Retrieval Buffer, pH8.0
  • Antigen Retrieval Buffer, pH8.0
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF025CPdatasheet pdfdatasheet pdf50 ml
    BUF025C
    BUF025BPdatasheet pdfdatasheet pdf500 ml
    BUF025B
    BUF025APdatasheet pdfdatasheet pdf500 ml
    BUF025A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Bio-Rad also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
      Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Bio-Rad also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
      Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The 'universal' formulation presented at pH8.0 is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Bio-Rad also offers two other versions, an acidic and basic form, which may be of value for use with antibodies that do not work well with this reagent. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
    • Product Form
    • Reconstitution
    • Preparation
      Mix 10ml with 90ml distilled water
      Mix 10ml with 990ml distilled water
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10x
      100x
      Ready to use
    • Storage
      Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1. Deparaffinize sections and re-hydrate

      2. Pre-heat retrieval buffer to 92oC *.

      3. Place slides in buffer and incubate 10 minutes at 92oC *.

      4. Allow to cool for 5-10 minutes.

      5. Rinse in distilled water or PBS, proceed with staining.

      * N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
    • Instructions For Use
      1. Deparaffinise sections and re-hydrate

      2. Pre-heat retrieval buffer to 92oC *.

      3. Place slides in buffer and incubate 10 minutes at 92oC *.

      4. Allow to cool for 5-10 minutes.

      5. Rinse in distilled water or PBS, proceed with staining.

      * N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.
    • Instructions For Use
      1. Deparaffinize sections and re-hydrate

      2. Pre-heat retrieval buffer to 92oC *.

      3. Place slides in buffer and incubate 10 minutes at 92oC *.

      4. Allow to cool for 5-10 minutes.

      5. Rinse in distilled water or PBS, proceed with staining.

      * N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.

    Additional Antigen Retrieval Buffer, pH8.0 Formats

    Formats Applications Sizes available
    Antigen Retrieval Buffer, pH8.0 : 100 X Concentrate P 50 ml
    Antigen Retrieval Buffer, pH8.0 : 10 X Concentrate P 500 ml
    Antigen Retrieval Buffer, pH8.0 : Ready To Use P 500 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH10.0BUF026B500 mlP
          BUF026B
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025B500 mlP
          BUF025B
          Antigen Retrieval Buffer, pH10.0BUF026C50 mlP
          BUF026C
          Antigen Retrieval Buffer, pH4.8BUF027C50 mlP
          BUF027C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025B500 mlP
          BUF025B
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH10.0BUF026A500 mlP
          BUF026A
          Antigen Retrieval Buffer, pH4.8BUF027A500 mlP
          BUF027A

          Recommended Positive Controls

            Histology Controls

              References

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