TNF Alpha antibody | VPM61
Mouse anti Sheep TNF alpha, clone VPM61 (MCA905GA) used to evaluate THF-&aplha; expression in ovine brain derived cell samples by flow cytometry.
Image caption:
Representative fluorograms obtained after flow cytometry analysis showing ROS, TNF-α, IL-1β, and IL-6 fluorescence. Samples of different brain regions from both hemispheres were analyzed in triplicate by flow cytometry (n = 6) for each animal and condition. Black lines correspond to auto-fluorescence, whereas grey areas correspond to positive cells. Sham, n = 6; HI+VEH, n = 6; HI+WIN, n = 6.
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep TNF alpha, clone VPM61 (MCA905GA) used to evaluate TNF-&aplha; expression in ovine brain by immunofluorescence.
Image caption:
Representative microphotographs 3 h after HI TNF-α (a,b,c), IL-1β (d,e,f), and IL-6 (g,h,i) positive cells after immunofluorescence labeling. Cortex brain sections were obtained after sham operation (a,d,g), HI+vehicle (b,e,h) or HI+WIN treatment (c,f,i). The histological evaluation reveals the presence of these cytokines (arrows) in asphyctic animals. Original magnification: ×200.
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep TNF alpha, clone VPM61 (MCA905GA) used to evaluate TNF-&aplha; expression in ovine brain by flow cytometry.
Image caption:
Evaluation of TNF-α expression in different brain regions evaluated after sham or HI (and vehicle or WIN treatment) in fetal lambs. Samples from both hemispheres were analyzed in triplicate by flow cytometry (n = 6) for each animal and condition. Values given as the mean ± SD of the percentage of TNF-α positive cells. (*) ANOVA p <0.05 vs. sham and HI+WIN; (**) ANOVA p <0.01 vs. sham and HI+WIN; (#) ANOVA p <0.05 vs. HI+VEH. Sham, n = 6; HI+VEH, n = 6; HI+WIN, n = 6. (C: cortex; BN: basal nuclei; HT: hypothalamus; T: thalamus; HC: hippocampus; CB: cerebellum).
From:Alonso-Alconada D, Álvarez FJ, Goñi-de-Cerio F, Hilario E, Álvarez A.
Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 2020 Feb 14;21(4):1283.
doi: 10.3390/ijms21041283.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep TNF Alpha
- Product Type
- Monoclonal Antibody
- Clone
- VPM61
- Isotype
- IgG2a
- Specificity
- TNF Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Sheep TNF alpha, clone VPM61 recognizes ovine tumor necrosis factor (TNF) alpha, a 17.5kDa cytokine expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumor and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant ovine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
||
| Immunohistology - Frozen | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for TNF Alpha antibody
-
Green, I.R. et al. (1993) Expression and characterization of bioactive recombinant ovine TNF-alpha: some species specificity in cytotoxic response to TNF.
Cytokine. 5 (3): 213-23. -
Vilmos, P. et al. (1996) Phylogenetically conserved epitopes of leukocyte antigens.
Vet Immunol Immunopathol. 52 (4): 415-26. -
Alonso-Alconada, D. et al. (2020) Cannabinoid-mediated Modulation of Oxidative Stress and Early Inflammatory Response after Hypoxia-Ischemia.
Int J Mol Sci. 21 (4): 1283.
Further Reading
-
Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.
Vet Immunol Immunopathol. 135 (3-4): 320-4. -
Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
- UniProt
- P23383
- Entrez Gene
- TNF
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
MCA905GA
152890
167017
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