CD45 antibody | 1.11.32
Mouse anti Bovine CD21 monoclonal antibody, clone CC21 (MCA1424GA) used to identify ovine CD21 expressing cells in heart tissue by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescence examination of explanted heart valves. CD45‐positive cells could be found within (A) allogeneic and (B) xenogeneic explants. C, Allogeneic explants exhibited the lowest number of CD45‐positive cells, whereas xenogeneic (xeno) SDS/SD‐treated explants exhibited the highest number, about 40% of all invading cells. High numbers of CD11b‐positive cells were present in all (D) allogeneic and (E) xenogeneic explants; however, (F) no significant differences were found. G, In allogeneic explants, CD3‐positive T cells were almost absent, but in (H) xenogeneic explants, a low number of CD3‐positive cells could be found. I, Enzyme‐treated valves tended to contain less CD3‐positive cells as untreated explants. J, In allogeneic as well (K) xenogeneic explants, (L) only few CD21‐positive B‐cells could be found. C, F, I, L, Every dot represents one individual animal included in the analysis. For each PHV specimen, at least two sections obtained from different areas have been analyzed, counting 11 HPFs per section. All scale bars represent 100 μm.
From: Ramm R, Goecke T, Theodoridis K, Hoeffler K, Sarikouch S, Findeisen K, Ciubotaru A, Cebotari S, Tudorache I, Haverich A, Hilfiker A.
Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 2020 Mar;27(2):e12571.
doi: 10.1111/xen.12571.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Sheep CD45 antibody, clone 1.11.32 (MCA2220F) used to evaluate CD45 expression on sheep cells by flow cytometry
Image caption:
Flow cytometry expression analysis.
Labeling of A: AT-Muse cells and B: ASCs with antibodies anti- SSEA3, CD45, CD44, CD166 and controls.
From: Castillo MG, Peralta TM, Locatelli P, Velazquez C, Herrero Y, Crottogini AJ, et al. (2023)
Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
PLoS ONE 18(1): e0277442.
doi:10.1371/journal.pone.0277442
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F C P IF ResetMouse anti Sheep CD45
- Product Type
- Monoclonal Antibody
- Clone
- 1.11.32
- Isotype
- IgG1
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD45 antibody, clone 1.11.32 recognizes the ovine CD45 (Leucocyte common antigen), expressed on all ovine lymphocytes, macrophages and granulocytes. Mouse anti Sheep CD45 antibody, clone 1.11.32 immunoprecipitates CD45 molecules of 190 kDa, 210 kDa and 225 kDa from lymph node lysates. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine efferent lymphatic duct lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Source Reference
-
Maddox, J.F. et al. (1985) The sheep analogue of leucocyte common antigen (LCA).
Immunology. 55 (2): 347-53.
References for CD45 antibody
-
Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. -
Zannettino, A.C. et al. (2010) Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
Tissue Eng Part A. 16 (12): 3579-87. -
Mackay, C.R. et al. (1989) Gamma/delta T cells express a unique surface molecule appearing late during thymic development.
Eur J Immunol. 19 (8): 1477-83. -
Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113 (4): 416-22. -
Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations.
Comp Immunol Microbiol Infect Dis. 34: 217-25. -
Herdrich, B.J. et al. (2010) Regenerative healing following foetal myocardial infarction.
Eur J Cardiothorac Surg. 38: 691-8. -
Reichert, J.C. et al. (2010) Ovine bone- and marrow-derived progenitor cells and their potential for scaffold-based bone tissue engineering applications in vitro and in vivo.
J Tissue Eng Regen Med. 4: 565-76. -
Galinsky, R. et al. (2011) Effect of intra-amniotic lipopolysaccharide on nephron number in preterm fetal sheep.
Am J Physiol Renal Physiol. 301 (2): F280-5.
View The Latest Product References
-
Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. -
Stickler, P. et al. (2010) Cyclically stretching developing tissue in vivo enhances mechanical strength and organization of vascular grafts.
Acta Biomater. 6 (7): 2448-56. -
Berardinelli, P. et al. (2013) Role of amniotic fluid mesenchymal cells engineered on MgHA/collagen-based scaffold allotransplanted on an experimental animal study of sinus augmentation.
Clin Oral Investig. 17 (7): 1661-75. -
Geherin, S.A. et al. (2012) The skin, a novel niche for recirculating B cells.
J Immunol. 188: 6027-35. -
Jahroomishirazi, R. et al. (2014) Isolation and Characterization of CD271+ Stem Cells Derived from Sheep Dermal Skin.
Cells Tissues Organs. 200 (2): 141-52. -
Stephens, E.H. et al. (2016) Extracellular matrix remodeling in wound healing of critical size defects in the mitral valve leaflet.
Heart Vessels. 31 (7): 1186-95. -
Mokhtari, S. et al. (2016) Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation.
Stem Cell Reports. 6 (6): 957-69. -
Bischoff, J. et al. (2016) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction.
Circ Res. 119 (11): 1215-25. -
Lydon, H. et al. (2018) Peripheral mononuclear blood cell apheresis in a preclinical ovine model.
BMC Vet Res. 14 (1): 47. -
Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
Viruses. 12 (3)Mar 07 [Epub ahead of print]. -
Barboni, B. et al. (2013) Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.
PLoS One. 8 (5): e63256. -
Ramm, R. et al. (2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model.
Xenotransplantation. 27 (2): e12571. -
López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
Tissue Eng Part A. 26 (17-18): 993-1004. -
Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-4262. -
Savy, V. et al. (2021) Effect of Embryo Aggregation on In Vitro Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones.
Cell Reprogram. 23 (5): 277-89. -
Castillo, M.G. et al. (2023) Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
PLoS One. 18 (1): e0277442.
- Synonyms
- LCA
- RRID
- AB_11152601
MCA2220GA
161823
1804
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