CD43 antibody | CO.44B8
Mouse anti Sheep CD43
- Product Type
- Monoclonal Antibody
- Clone
- CO.44B8
- Isotype
- IgG1
- Specificity
- CD43
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD43 antibody, clone CO.44B8 recognizes ovine CD43, also known as leukosialin or Sialophorin. CD43 is a ~105 kDa single pass type-1 transmembrane glycoprotein expressed by leucocytes. CD43 appears to have a role in cell aggregation (Pintado and Llanes 1995). |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Sheep leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused the cells of the SP2-0/Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for CD43 antibody
-
Brodesen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species
Vet Immunol Immunopathol. 64: 1-13. -
Pintado, C.O. and Llanes, D. (1995) Homotypic aggregation of ovine leucocyte induced by anti-leucosialin (CD43) monoclonal antibodies.
Vet Immunol Immunopathol. 47: 333-40. -
Schwartz-Cornil, I. et al. (1997) Bovine leukaemia virus-induced lymphocytosis in sheep is associated with reduction of spontaneous B cell apoptosis.
J Gen Virol. 78: 153-62. -
Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
PLoS One. 17 (12): e0278313.
- Synonyms
- Leukosialin
MCA1096GA
0215
169006
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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