CD32-Like antibody | VPM63

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F ResetMouse anti Sheep CD32-Like
- Product Type
- Monoclonal Antibody
- Clone
- VPM63
- Isotype
- IgG1
- Specificity
- CD32-Like
Mouse anti Sheep CD32-Like, clone VPM63 recognizes a cell surface antigen of 40-42 kDa which is similar to the human cell surface antigen designated as CD32. It has therefore been hypothesized that clone VPM63 may recognise a minor isoform of the ovine homologue of human CD32 (Hopkins et. al. 1996). CD32 is one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. CD32, also known as the Fc gamma RII, acts as a low affinity receptor for IgG. The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. Mouse anti Sheep CD32-Like, clone VPM63 has been shown to recognize an antigen expressed by macrophages and monocytes but not by lymphocytes and shows strong staining of alveolar macrophages in flow cytometry. In addition to clone VPM63, clone CCG36, an antibody directed against bovine CD32 but cross reacting with ovine CD32 is also available. Clone CCG36 is available in both unconjugated and conjugated formats. |
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine macrophages.
- Approx. Protein Concentrations
- IgG concentration1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/25 | 1/200 |
Immunohistology - Frozen | ![]() |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse anti Bovine CD32:FITC | MCA5919F | F | 0.1 mg |
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Description | Mouse anti Bovine CD32:FITC |
References for CD32-Like antibody
-
Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.
Vet Immunol Immunopathol. 52 (4): 329-39.
- Synonyms
- FcRII
MCA918GA


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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