CD230 antibody | ROS-BH1
Mouse anti SheepCD230 antibody, clone ROS-BH1 (MCA6008GA) used to cisualize prion protein deposition in scrapie infected mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
ROS-BH1 stains PrPd deposited after 87V infection in mice in a more sensitive manner compared to 6H4.
Staining of PrPSc during 87V (scrapie) infection in the cortical hippocampus, the cortex and the CA2 region of the hippocampus in mice using ROS-BH1 (0.2 μg/ml, panels A, D and G), 6H4 (3.0 μg/ml, panels B, E and F) and the IgG1 isotype control antibody, TNP (0.2 μg/ml, panels C, F and I). Panels, G H and I are higher magnification images of the CA2 region. Neuroanatomical landmarks are identified as follows: Fi – fimbria; Th - thalamus; DG - dentate gyrus; CA2 - CA2 region of the hippocampus; CA1 - CA1 region of the hippocampus; Cx - cortex; V - ventricle and S - septum. Scale bars are indicated in µm. ROS-BH1 gave more sensitive detection of PrPd in this assay compared to 6H4 whilst retaining an identical specificity and profile of staining to that of 6H4.
From: McCutcheon S, Langeveld JPM, Tan BC, Gill AC, de Wolf C, Martin S, et al. (2014)
Prion Protein-Specific Antibodies that Detect Multiple TSE Agents with High Sensitivity.
PLoS ONE 9(3): e91143.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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F WB P ResetMouse anti Sheep CD230 (aa143-154)
- Product Type
- Monoclonal Antibody
- Clone
- ROS-BH1
- Isotype
- IgG1
- Specificity
- CD230
- Region
- (aa143-154)
Antibody Quality and 
Unrivaled 
| Mouse anti Sheep CD230 (Prpsc) antibody, clone ROS-BH1 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals, and known as Scrapie (sheep and goats), BSE (cattle), CWD (cervine/deer ) and CJD (humans). These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioral changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. Also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Human Hamster Mouse Red deer - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A truncated form of recombinant Ovine PRP spanning residues aa94 - aa233.
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised 129/Ola mice were fused with cells of the SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was licenced from The Roslin Institute at the University of Edinburgh as part of the immunological toolbox project
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunohistology - Paraffin | |
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| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Source Reference
-
Mccutcheon, S. et al. (2014) Prion protein-specific antibodies that detect multiple TSE agents with high sensitivity.
PLoS One. 9 (3): e91143.
- Synonyms
- PRP
- UniProt
- P23907
- P10279
- Entrez Gene
- PRNP
- PRNP
- GO Terms
- GO:0005886 plasma membrane
- GO:0005507 copper ion binding
- GO:0005794 Golgi apparatus
- GO:0008017 microtubule binding
- GO:0031225 anchored to membrane
- GO:0051260 protein homooligomerization
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