CD16-Like antibody | VPM64

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Mouse anti Sheep CD16-Like

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA919GA C E F IP 0.1 mg
Mouse anti Sheep CD16-Like, clone VPM64 recognizes a cell surface antigen of 65-80kDa which is similar to the human cell surface antigen designated as CD16. It has therefore been hypothesized that clone VPM64 may recognize the ovine homologue of human CD16 (Hopkins, J. et. al. 1996).

Clone VPM64 recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.

In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad. Clone KD1 is available purified and conjugated to FITC.

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide.
Carrier Free
Ovine macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch

More Information

For research purposes only

Applications of CD16-Like antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500
Flow Cytometry 1/25 1/200
Immunohistology - Frozen Neat
Immunoprecipitation Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Human CD16:FITC MCA5665F 0.1 mg F

Product Specific References

References for CD16-Like antibody

  1. Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.
    Vet Immunol Immunopathol. 52 (4): 329-39.
  2. Caroprese, M. et al. (2008) Technical note: immunomagnetic procedure for positive selection of macrophages in ovine milk.
    J Dairy Sci. 91:1908-12.
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