CD16-Like antibody | VPM64
Mouse anti Sheep CD16-Like, clone VPM64 recognizes a cell surface antigen of 65-80kDa which is similar to the human cell surface antigen designated as CD16. It has therefore been hypothesized that clone VPM64 may recognize the ovine homologue of human CD16 (Hopkins, J. et. al. 1996).
Clone VPM64 recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.
In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad. Clone KD1 is available purified and conjugated to FITC.
Clone VPM64 recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.
In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad. Clone KD1 is available purified and conjugated to FITC.
Product Details
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide.
- Carrier Free
- Yes
- Immunogen
- Ovine macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD16-Like antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | ||
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | Neat | ||
| Immunoprecipitation | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Human CD16:FITC | MCA5665F | F | 0.1 mg |
|
Product Specific References
References for CD16-Like antibody
-
Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.
Vet Immunol Immunopathol. 52 (4): 329-39. -
Caroprese, M. et al. (2008) Technical note: immunomagnetic procedure for positive selection of macrophages in ovine milk.
J Dairy Sci. 91:1908-12.
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