CD16-Like antibody | VPM64
Clone VPM64 recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.
In addition to clone VPM64, clone KD1, an antibody directed against human CD16 and cross reacting with ovine and bovine CD16 is also available from Bio-Rad. Clone KD1 is available purified and conjugated to FITC.
Product Details
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide.
- Carrier Free
- Yes
- Immunogen
- Ovine macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD16-Like antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/500 | ||
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | Neat | ||
Immunoprecipitation | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Human CD16:FITC | MCA5665F | F | 0.1 mg |
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Product Specific References
References for CD16-Like antibody
-
Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.
Vet Immunol Immunopathol. 52 (4): 329-39. -
Caroprese, M. et al. (2008) Technical note: immunomagnetic procedure for positive selection of macrophages in ovine milk.
J Dairy Sci. 91:1908-12.