CD14 antibody | VPM65

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Mouse anti Sheep CD14

Product Type
Monoclonal Antibody
Clone
VPM65
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA920GA C E F IP 0.1 mg
Mouse Anti Sheep CD14 antibody, clone VPM65 recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes.

CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine macrophages
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q06AV9 Related reagents
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/250
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD14 antibody

  1. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  2. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
    Vet Immunol Immunopathol. 51 (1-2): 89-99.
  3. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  4. Langrova, T. et al. (2008) Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide
    Veterinarni Medicina, 53: 1–11
  5. Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.
    J Histochem Cytochem. 50: 1357-70.
  6. Berthon, P. and Hopkins, J. (1996) Ruminant cluster CD14.
    Vet Immunol Immunopathol. 52: 245-8.
  7. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107 (3): 366-72.
  8. Daniel,. J.A. et al. (2003) Leptin, tumor necrosis factor-alpha (TNF), and CD14 in ovine adipose tissue and changes in circulating TNF in lean and fat sheep.
    J Anim Sci. 81: 2590-9.
  9. Faldyna, M. et al. (1999) Leukocyte counts and lymphocyte subpopulations in the peripheral blood of pygmy goats from herd infected with Mycobacterium avium subspecies paratuberculosis
    Veterinarni Medicina 44: 259-62
  10. González, L. et al. (2001) Detection of immune system cells in paraffin wax-embedded ovine tissues.
    J Comp Pathol. 125: 41-7.
  11. Merkwitz, C. et al. (2013) Dual origin, development, and fate of bovine pancreatic islets.
    J Anat. 222: 358-71.
  12. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
    Immunology. 113: 310-7.
  13. Ryan, S. et al. (2000) Infection of dendritic cells by the Maedi-Visna lentivirus.
    J Virol. 74: 10096-103.
  14. Sladek, Z. and Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
    Acta Vet Scand. 52: 12.
  15. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  16. Roperto, S. et al. (2011) PBMCs are additional sites of productive infection of bovine papillomavirus type 2.
    J Gen Virol. 92: 1787-94.
  17. Spanel-Borowski, K. et al. (1997) Immunolocalization of CD18-positive cells in the bovine ovary.
    J Reprod Fertil. 111: 197-205.
  18. Zladek,Z and Rysanek,D. (2008) Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide
    Veterinarni Medicina: 53: 347–57
  19. Sladek, Z. et al. (2001) Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx
    Vet. Med. Czech, 46: 190–98
  20. Vilmos, P., et. al (1996) Phylogenetically conserved epitopes of leukocyte antigens
    Vet Immunol Immunopath. 52: 415-426
  21. Bischoff, J. et al. (2016) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction.
    Circ Res. 119 (11): 1215-25.
  22. Sladek, Z. & Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
    Acta Vet Scand. 52: 12.
  23. Douet, J.Y. et al. (2016) Mononucleated Blood Cell Populations Display Different Abilities To Transmit Prion Disease by the Transfusion Route.
    J Virol. 90 (7): 3439-45.