CD14 antibody | VPM65

Mouse anti Sheep CD14

Product Type
Monoclonal Antibody
Clone
VPM65
Isotype
IgG1
Specificity
CD14

Product Code Applications Pack Size List Price Your Price Qty
MCA920GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IP 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse Anti Sheep CD14 antibody, clone VPM65 recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes.

CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine macrophages
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/250
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP

References for CD14 antibody

  1. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  2. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
    Vet Immunol Immunopathol. 51 (1-2): 89-99.
  3. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  4. Langrova, T. et al. (2008) Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide
    Veterinarni Medicina, 53: 1–11
  5. Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.
    J Histochem Cytochem. 50: 1357-70.
  6. Berthon, P. and Hopkins, J. (1996) Ruminant cluster CD14.
    Vet Immunol Immunopathol. 52: 245-8.
  7. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107 (3): 366-72.
  8. Daniel,. J.A. et al. (2003) Leptin, tumor necrosis factor-alpha (TNF), and CD14 in ovine adipose tissue and changes in circulating TNF in lean and fat sheep.
    J Anim Sci. 81: 2590-9.
  9. View The Latest Product References
  10. Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339 (1): 1-10.
  11. González, L. et al. (2001) Detection of immune system cells in paraffin wax-embedded ovine tissues.
    J Comp Pathol. 125: 41-7.
  12. Merkwitz, C. et al. (2013) Dual origin, development, and fate of bovine pancreatic islets.
    J Anat. 222: 358-71.
  13. Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
    Immunology. 113: 310-7.
  14. Ryan, S. et al. (2000) Infection of dendritic cells by the Maedi-Visna lentivirus.
    J Virol. 74: 10096-103.
  15. Sladek, Z. and Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
    Acta Vet Scand. 52: 12.
  16. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  17. Roperto, S. et al. (2011) PBMCs are additional sites of productive infection of bovine papillomavirus type 2.
    J Gen Virol. 92: 1787-94.
  18. Spanel-Borowski, K. et al. (1997) Immunolocalization of CD18-positive cells in the bovine ovary.
    J Reprod Fertil. 111: 197-205.
  19. Zladek,Z and Rysanek,D. (2008) Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide
    Veterinarni Medicina: 53: 347–57
  20. Sladek, Z. et al. (2001) Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx
    Vet. Med. Czech, 46: 190–98
  21. Vilmos, P., et. al (1996) Phylogenetically conserved epitopes of leukocyte antigens
    Vet Immunol Immunopath. 52: 415-426
  22. Bischoff, J. et al. (2016) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction.
    Circ Res. 119 (11): 1215-25.
  23. Sladek, Z. & Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
    Acta Vet Scand. 52: 12.
  24. Douet, J.Y. et al. (2016) Mononucleated Blood Cell Populations Display Different Abilities To Transmit Prion Disease by the Transfusion Route.
    J Virol. 90 (7): 3439-45.
  25. Zavadilova, T. (2019) The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 and CD44 by Mammary Gland Neutrophils in vitro
    J Microbiol, Biotechnol and Food Sci. 9 (Special issue): 467-72.
  26. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  27. Pedro, A.R.V. et al. (2021) Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes.
    Front Immunol. 12: 689879.

Flow Cytometry

UniProt
Q06AV9
View more products with CD14 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Sheep Products