CD14 antibody | VPM65
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F ResetMouse anti Sheep CD14
- Product Type
- Monoclonal Antibody
- Clone
- VPM65
- Isotype
- IgG1
- Specificity
- CD14
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Antibody Quality and 
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| Mouse Anti Sheep CD14 antibody, clone VPM65 recognizes Ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, which has been shown to upregulate the cell surface expression of adhesion molecules. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine macrophages
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/250 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood
References for CD14 antibody
-
Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
Vet Immunol Immunopathol. 51 (1-2): 89-99. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Langrova, T. et al. (2008) Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide
Veterinarni Medicina, 53: 1–11 -
Andréoletti, O. et al. (2002) Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry.
J Histochem Cytochem. 50: 1357-70. -
Berthon, P. and Hopkins, J. (1996) Ruminant cluster CD14.
Vet Immunol Immunopathol. 52: 245-8. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107 (3): 366-72. -
Daniel,. J.A. et al. (2003) Leptin, tumor necrosis factor-alpha (TNF), and CD14 in ovine adipose tissue and changes in circulating TNF in lean and fat sheep.
J Anim Sci. 81: 2590-9.
View The Latest Product References
-
Foulon, E. & Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339 (1): 1-10. -
González, L. et al. (2001) Detection of immune system cells in paraffin wax-embedded ovine tissues.
J Comp Pathol. 125: 41-7. -
Merkwitz, C. et al. (2013) Dual origin, development, and fate of bovine pancreatic islets.
J Anat. 222: 358-71. -
Newland, A. et al. (2004) Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.
Immunology. 113: 310-7. -
Ryan, S. et al. (2000) Infection of dendritic cells by the Maedi-Visna lentivirus.
J Virol. 74: 10096-103. -
Sladek, Z. and Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
Acta Vet Scand. 52: 12. -
Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
J Virol. 80: 7873-84. -
Roperto, S. et al. (2011) PBMCs are additional sites of productive infection of bovine papillomavirus type 2.
J Gen Virol. 92: 1787-94. -
Spanel-Borowski, K. et al. (1997) Immunolocalization of CD18-positive cells in the bovine ovary.
J Reprod Fertil. 111: 197-205. -
Zladek,Z and Rysanek,D. (2008) Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide
Veterinarni Medicina: 53: 347–57 -
Sladek, Z. et al. (2001) Leukocytes in bovine virgin mammary gland: flow cytometry imaging during development and resolution of induced influx
Vet. Med. Czech, 46: 190–98 -
Vilmos, P., et. al (1996) Phylogenetically conserved epitopes of leukocyte antigens
Vet Immunol Immunopath. 52: 415-426 -
Bischoff, J. et al. (2016) CD45 Expression in Mitral Valve Endothelial Cells After Myocardial Infarction.
Circ Res. 119 (11): 1215-25. -
Sladek, Z. & Rysanek, D. (2010) Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.
Acta Vet Scand. 52: 12. -
Douet, J.Y. et al. (2016) Mononucleated Blood Cell Populations Display Different Abilities To Transmit Prion Disease by the Transfusion Route.
J Virol. 90 (7): 3439-45. -
Zavadilova, T. (2019) The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 and CD44 by Mammary Gland Neutrophils in vitro
J Microbiol, Biotechnol and Food Sci. 9 (Special issue): 467-72. -
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Pedro, A.R.V. et al. (2021) Dectin-1-Mediated Production of Pro-Inflammatory Cytokines Induced by Yeast β-Glucans in Bovine Monocytes.
Front Immunol. 12: 689879.
- UniProt
- Q06AV9
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