TCR Gamma/Delta antibody | V65
Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor.
Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.
Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.
Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.
Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Prosep A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of TCR Gamma/Delta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
|
Product Specific References
References for TCR Gamma/Delta antibody
-
Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 153 (3): 979-86. -
Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
Dev Immunol. 4 (3): 181-8. -
Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
J Immunol. 177: 6115-21.
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