TCR Gamma/Delta antibody | V65

Mouse anti Rat TCR Gamma/Delta:RPE

Product Type
Monoclonal Antibody
Clone
V65
Isotype
IgG1
Specificity
TCR Gamma/Delta

Product Code Applications Pack Size List Price Your Price Qty
MCA1146PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.

Target Species
Rat
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:RPE

References for TCR Gamma/Delta antibody

  1. Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
    J Immunol. 153 (3): 979-86.
  2. Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
    Dev Immunol. 4 (3): 181-8.
  3. Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
    J Immunol. 177: 6115-21.

RRID
AB_322446

MCA1146PE

INN0515

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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