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TCR Gamma/Delta antibody | V65

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Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 1 Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 2 Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 3
Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 1
Figure A. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse anti Rat TCR gamma/delta (MCA1146PE). All experiments performed on rat splenocytes gated on live, single cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer
Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 2
Figure A. RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE) and FITC conjugated Mouse anti Rat CD43 (MCA54FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells
Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 3
Figure A. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse anti Rat TCRg/d (MCA1146PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells

Mouse anti Rat TCR Gamma/Delta

Mouse anti Rat TCR Gamma/Delta:RPE

Product Type
Monoclonal Antibody
Clone
V65
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1146GA
Datasheet
C F IP
SDS
0.1 mg loader

MCA1146
Datasheet
C F IP
SDS
0.25 mg loader

MCA1146PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Prosep A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of TCR Gamma/Delta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader

Product Specific References

References for TCR Gamma/Delta antibody

  1. Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
    J Immunol. 153 (3): 979-86.
  2. Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
    Dev Immunol. 4 (3): 181-8.
  3. Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
    J Immunol. 177: 6115-21.

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