TCR Gamma/Delta antibody | V65

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Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 1

Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 2

Anti Rat TCR Gamma/Delta Antibody, clone V65 thumbnail image 3

Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 1 — Figure A. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse anti Rat TCR gamma/delta (**MCA1146PE**). All experiments performed on rat splenocytes gated on live, single cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer

Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 2 — Figure A. RPE conjugated Mouse anti Rat TCRg/d (**MCA1146PE**) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated Mouse anti Rat TCRg/d (**MCA1146PE**) and FITC conjugated Mouse anti Rat CD43 (MCA54FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Anti Rat TCR Gamma/Delta Antibody, clone V65 gallery image 3 — Figure A. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD43 (MCA54FT) and RPE conjugated Mouse anti Rat TCRg/d (**MCA1146PE**). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

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Mouse anti Rat TCR Gamma/Delta:RPE

Product Type: Monoclonal Antibody
Clone: V65
Isotype: IgG1
Specificity: TCR Gamma/Delta

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References (3)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA1146PE Datasheet Datasheet SDS SDS	F	100 Tests		Log in	Get Quote
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Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes.

Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4.

Target Species: Rat
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
5% sucrose
Immunogen: CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:RPE	MCA1209PE	F	100 Tests	Log in	Get Quote
List Price	Your Price
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Description	Mouse IgG1 Negative Control:RPE

References for TCR Gamma/Delta antibody

Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 153 (3): 979-86.
Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
Dev Immunol. 4 (3): 181-8.
Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
J Immunol. 177: 6115-21.