TCR Gamma/Delta antibody | V65
Mouse anti Rat TCR Gamma/Delta
- Product Type
- Monoclonal Antibody
- Clone
- V65
- Isotype
- IgG1
- Specificity
- TCR Gamma/Delta
Antibody Quality and Performance Guarantee
| Mouse anti Rat TCR Gamma/Delta antibody, clone V65 recognizes a constant determinant of the rat gamma/delta T cell receptor. Mouse anti Rat TCR Gamma/Delta antibody, clone V65 stains all rat gamma/delta T cells, and also identifies as gamma/delta T cells a population of dendritic epidermal T lymphocytes. Mouse anti Rat TCR Gamma/Delta antibody, clone V65 may be used to stimulate the proliferation of gamma/delta T cells in the presence of either IL-2 or IL-4. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Prosep A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CD3 +ve TCR alpha/beta -ve rat T cell hybridoma line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag:8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Kühnlein, P. et al. (1994) Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor.
J Immunol. 153 (3): 979-86.
References for TCR Gamma/Delta antibody
-
Kühnlein, P. et al. (1995) Gamma/delta T cells in fetal, neonatal, and adult rat lymphoid organs.
Dev Immunol. 4 (3): 181-8. -
Yrlid, U. et al. (2006) Plasmacytoid dendritic cells do not migrate in intestinal or hepatic lymph.
J Immunol. 177: 6115-21. -
Martin, A. et al. (2018) Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
Oncotarget. 9 (47): 28364-78.
- RRID
- AB_322446
MCA1146
149734
166402
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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