IgD antibody | MARD-3
Biotinylated Mouse anti Rat IgD antibody, clone MARD-3 (MCA190B) used for the evaluation of IgD expression on rat splenocytes by flow cytometry.
Image caption:
Three-colour cytometry was used to analyze FO-B cells and MZ-B cells.
A single neonatal rat splenic cell suspension was stained with FITC conjugated anti-rat IgM (HIS40), biotinylated anti-rat IgD (MaRD3; AbD Serotec, Oxford, UK), and APC anti-rat CD90/Thy1.1 (HIS51). Biotinylated mAb were revealed with streptavidin conjugated to the tandem fluorochrome PE-Cy5.5. Lymphocytes were sequentially gated by forward scatter and side scatter. Acquisition gates were set to exclude the unwanted immature B cells (CD90+-APC). Gate settings were set appropriately for FO-B cells (CD90negIgDhighIgMlow) and MZ-B (CD90negIgMhighIgDlow) cells.
From: Hendricks J, Visser A, Dammers PM, Burgerhof JGM, Bos NA, Kroese FGM (2019)
The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.
PLoS ONE 14(9): e0220933.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat IgD
- Product Type
- Monoclonal Antibody
- Clone
- MARD-3
- Isotype
- IgG1
- Specificity
- IgD
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgD antibody, clone MARD-3 recognizes rat IgD, and does not cross-react with other rat immunoglobulin classes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- IR731 myeloma protein.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunized BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent.
References for IgD antibody
-
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82. -
Westermann, J. et al. (2005) Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells.
J Immunol. 174: 2517-24. -
FrancoSalinas, G. et al. (2011) TNF blockade abrogates the induction of T cell-dependent humoral responses in an allotransplantation model.
J Leukoc Biol. 90 (2): 367-75. -
Zonneveld-Huijssoon, E. et al. (2011) Bystander suppression of experimental arthritis by nasal administration of a heat shock protein peptide.
Ann Rheum Dis. 70: 2199-206. -
Shin, J. et al. (2015) Development and pharmacological validation of novel methods of B cell activation in rat whole blood.
J Pharmacol Toxicol Methods. 71: 61-7. -
Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15. -
Hendricks, J. et al. (2019) The formation of mutated IgM memory B cells in rat splenic marginal zones is an antigen dependent process.
PLoS One. 14 (9): e0220933. -
Cai, Y. et al. (2024) Preclinical Pharmacology Characterization of Sovleplenib (HMPL-523), an Orally Available Syk Inhibitor.
J Pharmacol Exp Ther. 388 (1): 156-70.
View The Latest Product References
-
Ménoret, S. et al. (2020) In Vivo Analysis of Human Immune Responses in Immunodeficient Rats.
Transplantation. 104 (4): 715-23.
Further Reading
-
Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8.
- RRID
- AB_322200
- UniProt
- P01883
- Entrez Gene
- LOC641523
- GO Terms
- GO:0003823 antigen binding
MCA190
148513
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