IgD antibody | MARD-3

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Mouse anti Rat IgD:Biotin

Mouse anti Rat IgD

Product Type
Monoclonal Antibody
Clone
MARD-3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA190B E 0.5 mg
MCA190 C E 0.25 mg
Mouse anti Rat IgD antibody, clone MARD-3 recognizes rat IgD, and does not cross-react with other rat immunoglobulin classes.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
IR731 myeloma protein.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunized BALB/c mouse were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01883 Related reagents
Entrez Gene
LOC641523 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 250 ng/ml
ELISA
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent.

Product Specific References

References for IgD antibody

  1. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J Immunol. 121 (5): 2077-82.
  2. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  3. Westermann, J. et al. (2005) Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells.
    J Immunol. 174: 2517-24.
  4. FrancoSalinas, G. et al. (2011) TNF blockade abrogates the induction of T cell-dependent humoral responses in an allotransplantation model.
    J Leukoc Biol. 90 (2): 367-75.
  5. Zonneveld-Huijssoon, E. et al. (2011) Bystander suppression of experimental arthritis by nasal administration of a heat shock protein peptide.
    Ann Rheum Dis. 70: 2199-206.
  6. Shin, J. et al. (2015) Development and pharmacological validation of novel methods of B cell activation in rat whole blood.
    J Pharmacol Toxicol Methods. 71: 61-7.
  7. Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.