IFN Gamma antibody | DB-1

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Mouse anti Rat Interferon Gamma

Product Type
Monoclonal Antibody
Clone
DB-1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1301GA
Datasheet
C E F
MSDS
0.1 mg loader

MCA1301
Datasheet
C E F WB
MSDS
0.25 mg loader

Mouse anti Rat Interferon gamma antibody, clone DB-1 reacts with both mouse and rat Interferon gamma. Clone DB-1 exhibits high affinity binding to the natural and recombinant forms and effectively neutralizes the anti viral activity of IFN-gamma. It also blocks the induction of Ia antigen expression on murine keratinocytes.

Mouse anti Rat Interferon gamma antibody, clone DB-1 does not cross-react with rodent IFN-alpha or IFN-beta or with human interferons.

Mouse anti Rat Interferon gamma antibody, clone DB-1 has also been reported for use in neutralising studies.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09%Sodium Azide
Pack Size: 0.25 mg
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant rat IFN gamma.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01581 Related reagents
Entrez Gene
Ifng Related reagents
GO Terms
GO:0002026 regulation of the force of heart contraction
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0042493 response to drug
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032224 positive regulation of synaptic transmission, cholinergic
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045666 positive regulation of neuron differentiation
GO:0045785 positive regulation of cell adhesion
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 10ug/ml 100ug/ml
Flow Cytometry
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for IFN Gamma antibody

  1. Paineau, J. et al. (1991) Effect of recombinant interferon gamma and interleukin-2 and of a monoclonal antibody against interferon gamma on the rat immune response against heart allografts.
    J Heart Lung Transplant. 10 (3): 424-30.
  2. Paineau, J. et al.. (1989) Effects of gamma interferon and interleukin-2, and of gamma-interferon antibodies, on the rat immune response against allografts.
    Transplant Proc. 21: 999-1001.
  3. van der Meide, P.H. et al. (1986) The purification and characterization of rat gamma interferon by use of two monoclonal antibodies.
    J Gen Virol. 67 ( Pt 6): 1059-71.
  4. Keller, R. et al. (1987) Induction, maintenance, and reinduction of tumoricidal activity in bone marrow-derived mononuclear phagocytes by Corynebacterium parvum. Evidence for the involvement of a T cell- and interferon-gamma-independent pathway of macrophage activation.
    J Immunol. 138 (7): 2366-71.
  5. Jacob, C.O. et al. (1989) Heterogeneous effects of IFN-gamma in adjuvant arthritis.
    J Immunol. 142 (5): 1500-5.
  6. Kingston, A.E. et al. (1989) Schwann cells co-cultured with stimulated T cells and antigen express major histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction.
    Eur J Immunol. 19 (1): 177-83.
  7. Graf, M.R. et al. (2001) IL-6 secretion by a rat T9 glioma clone induces a neutrophil-dependent antitumor response with resultant cellular, antiglioma immunity.
    J Immunol. 166 (1): 121-9.
  8. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
    Blood. 102: 1764-70.
  9. Straube, F. and Herrmann, T. (2001) Differential modulation of CD8beta by rat gammadelta and alphabeta T cells after activation.
    Immunology. 104: 252-8.
  10. van der Meide PH et al. (1989) Isolation and characterization of monoclonal antibodies directed to rat interferon-gamma.
    Lymphokine Res. 8 (4): 439-49.