CD8 Beta antibody | 341
Mouse anti Rat CD8 beta antibody, clone 341 recognizes the beta chain of the rat CD8 cell surface antigen, expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. Mouse anti Rat CD8 beta antibody, clone 341 is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat/mouse T cell hybrids expressing CD8.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05541
- Entrez Gene
- Cd8b
- GO Terms
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of CD8 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/2 | 1/10 | |
| Flow Cytometry | 1/100 | 1/200 | |
| Immunofluorescence | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
References for CD8 Beta antibody
-
Pérez-Cano, F.J. et al. (2010) Influence of breast milk polyamines on suckling rat immune system maturation.
Dev Comp Immunol. 34: 210-8. -
Torres-nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression.
Eur J Immunol. 22 (11): 2841-8. -
Yan, Y. et al. (2003) Pathogenesis of Autoimmunity After Xenogeneic Thymus Transplantation
J Immunol. 170: 5936-46. -
Jörns, A. et al. (2010) Diabetes prevention by immunomodulatory FTY720 treatment in the LEW.1AR1-iddm rat despite immune cell activation.
Endocrinology. 151: 3555-65. -
Feferman, T. et al. (2005) Overexpression of IFN-induced protein 10 and its receptor CXCR3 in myasthenia gravis.
J Immunol. 174 (9): 5324-31.
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