CD63 antibody | AD1

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MCA4754GA
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SDS Safety Datasheet SDS
F IP P WB 0.1 mg loader
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loader
MCA4754T
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F IP P WB 25 µg loader
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Search for Batch Specific Datasheets

Mouse anti Rat CD63 antibody, clone AD1 recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell types in vitro (Nishikata et al. 1992).

Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitani et al. 1991).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
RBL-2H3 rat basophilic leukaemia cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/200
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Mast cells or basophils.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

Source Reference

  1. Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.
    J Biol Chem. 266 (3): 1903-9.

Antibody Characterization Reference

  1. Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.
    J Immunol. 149(3): 862-70. J Immunol. 149(3): 862-70.

References for CD63 antibody

  1. Kraft, S. et al. (2005) Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo.
    J Exp Med. 201: 385-96.
  2. Boros, M. et al. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat.
    Shock. 3 (2): 125-31.
  3. Vincent-Schneider, H. et al. (2001) Secretory granules of mast cells accumulate mature and immature MHC class II molecules
    J Cell Sci. 114(Pt 2): 323-34.
  4. Ramezani-Moghadam, M. et al. (2015) Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion.
    J Biol Chem. 290(9): 5533-42.
  5. Yoshimura, A. et al. (2016) Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids.
    Sci Rep. 6: 31172.
  6. Pasquier, A. et al. (2019) Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
    Nat Commun. 10 (1): 3312.

Immunoprecipitation

Western Blotting

RRID
AB_2076616
UniProt
P28648
Entrez Gene
Cd63
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0030658 transport vesicle membrane
GO:0010633 negative regulation of epithelial cell migration
GO:0030855 epithelial cell differentiation
GO:0032403 protein complex binding
GO:0043234 protein complex
GO:0045785 positive regulation of cell adhesion
GO:0045807 positive regulation of endocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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