CD5 antibody | OX-19
Mouse anti Rat CD5
- Product Type
- Monoclonal Antibody
- Clone
- OX-19
- Isotype
- IgG1
- Specificity
- CD5
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells. Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995). Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocyte glycoproteins.
- Approx. Protein Concentrations
- MCA52R: IgG concentration 1.0 mg/ml
- MCA52GA: IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from an immunized BALB/c mouse were fused with cells from the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
||
| Immunoprecipitation | |
- 1The use of PLP fixation is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their particular system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD5 antibody
-
Dallman, M.J. et al. (1982) The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells.
Eur J Immunol. 12 (6): 511-8. -
Dallman, M.J. et al. (1984) MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses.
Eur J Immunol. 14 (3): 260-7. -
Huitinga, I. et al. (1995) Macrophages in T cell line-mediated, demyelinating, and chronic relapsing experimental autoimmune encephalomyelitis in Lewis rats.
Clin Exp Immunol. 100 (2): 344-51. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Kikuchi, H. et al. (2000) Severe proteinuria, sustained for 6 months, induces tubular epithelial cell injury and cell infiltration in rats but not progressive interstitial fibrosis.
Nephrol Dial Transplant.15: 799-810. -
Antus, B. et al. (2001) Contribution of androgens to chronic allograft nephropathy is mediated by dihydrotestosterone
Kidney Int. 60: 1955-63. -
Song, E. et al. (2002) Early application of Met-RANTES ameliorates chronic allograft nephropathy.
Kidney Int. 61: 676-85. -
Shiraishi, H. et al. (2002) Antibody binding to fas ligand attenuates inflammatory cell infiltration and cytokine secretion, leading to reduction of myocardial infarct areas and reperfusion injury.
Lab Invest. 82: 1121-9.
View The Latest Product References
-
de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168: 5832-9. -
Moreno-Manzano, V. et al. (2003) Retinoids as a potential treatment for experimental puromycin-induced nephrosis
Br J Pharmacol. 139: 823-31. -
Antus, B. et al. (2005) Effects of progesterone and selective oestrogen receptor modulators on chronic allograft nephropathy in rats.
Nephrol Dial Transplant. 20: 329-35. -
Robichon, R. et al. (2005) Pig xenografts to the immunocompetent rat brain: Survival rates using distinct neurotoxic lesions in the nigrostriatal pathway and two rat strains.
Exp Neurol. 194: 333-40. -
Rusai, K. et al. (2008) Administration of interleukin-1 receptor antagonist ameliorates renal ischemia-reperfusion injury.
Transpl Int. 21: 572-80. -
Abrams, M.B. et al. (2009) Multipotent mesenchymal stromal cells attenuate chronic inflammation and injury-induced sensitivity to mechanical stimuli in experimental spinal cord injury.
Restor Neurol Neurosci. 27: 307-21. -
Pamukcu, O. et al. (2016) Anti-inflammatory role of obestatin in autoimmune myocarditis.
Clin Exp Pharmacol Physiol. 43 (1): 47-55.
- RRID
- AB_2075316
- UniProt
- P51882
- Entrez Gene
- Cd5
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0009966 regulation of signal transduction
- GO:0044214 fully spanning the plasma membrane
MCA52R
1609
MCA52GA
153107
154015
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