CD5 antibody | OX-19

Product Code Applications Pack Size List Price Your Price Qty
MCA52GA
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SDS Safety Datasheet SDS
C F IP P * 0.1 mg loader
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loader
MCA52R
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SDS Safety Datasheet SDS
C F IP P * 0.25 mg loader
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loader
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Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells.

Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995).

Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat thymocyte glycoproteins.
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml
Pack Size: 0.25 mg
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunized BALB/c mouse were fused with cells from the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1The use of PLP fixation is recommended for optimal results.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their particular system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD5 antibody

  1. Dallman, M.J. et al. (1982) The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells.
    Eur J Immunol. 12 (6): 511-8.
  2. Dallman, M.J. et al. (1984) MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses.
    Eur J Immunol. 14 (3): 260-7.
  3. Huitinga, I. et al. (1995) Macrophages in T cell line-mediated, demyelinating, and chronic relapsing experimental autoimmune encephalomyelitis in Lewis rats.
    Clin Exp Immunol. 100 (2): 344-51.
  4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  5. Song, E. et al. (2002) Early application of Met-RANTES ameliorates chronic allograft nephropathy.
    Kidney Int. 61: 676-85.
  6. Moreno-Manzano, V. et al. (2003) Retinoids as a potential treatment for experimental puromycin-induced nephrosis
    Br J Pharmacol. 139: 823-31.
  7. Shiraishi, H. et al. (2002) Antibody binding to fas ligand attenuates inflammatory cell infiltration and cytokine secretion, leading to reduction of myocardial infarct areas and reperfusion injury.
    Lab Invest. 82: 1121-9.
  8. Rusai, K. et al. (2008) Administration of interleukin-1 receptor antagonist ameliorates renal ischemia-reperfusion injury.
    Transpl Int. 21: 572-80.
  9. View The Latest Product References
  10. Antus, B. et al. (2001) Contribution of androgens to chronic allograft nephropathy is mediated by dihydrotestosterone
    Kidney Int. 60: 1955-63.
  11. Antus, B. et al. (2005) Effects of progesterone and selective oestrogen receptor modulators on chronic allograft nephropathy in rats.
    Nephrol Dial Transplant. 20: 329-35.
  12. Kikuchi, H. et al. (2000) Severe proteinuria, sustained for 6 months, induces tubular epithelial cell injury and cell infiltration in rats but not progressive interstitial fibrosis.
    Nephrol Dial Transplant.15: 799-810.
  13. Abrams, M.B. et al. (2009) Multipotent mesenchymal stromal cells attenuate chronic inflammation and injury-induced sensitivity to mechanical stimuli in experimental spinal cord injury.
    Restor Neurol Neurosci. 27: 307-21.
  14. Robichon, R. et al. (2005) Pig xenografts to the immunocompetent rat brain: Survival rates using distinct neurotoxic lesions in the nigrostriatal pathway and two rat strains.
    Exp Neurol. 194: 333-40.
  15. de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
    J Immunol. 168: 5832-9.
  16. Pamukcu, O. et al. (2016) Anti-inflammatory role of obestatin in autoimmune myocarditis.
    Clin Exp Pharmacol Physiol. 43 (1): 47-55.

Immunohistology - Frozen

RRID
AB_2075316
UniProt
P51882
Entrez Gene
Cd5
GO Terms
GO:0001948 glycoprotein binding
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0009966 regulation of signal transduction
GO:0044214 fully spanning the plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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