CD5 antibody | OX-19
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|Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells.
Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995).
Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat thymocyte glycoproteins.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 1.0mg/mlPack Size: 0.25 mgIgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunized BALB/c mouse were fused with cells from the NS1 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1The use of PLP fixation is recommended for optimal results.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD5 antibody
Dallman, M.J. et al. (1982) The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells.
Eur J Immunol. 12 (6): 511-8.
Dallman, M.J. et al. (1984) MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses.
Eur J Immunol. 14 (3): 260-7.
Huitinga, I. et al. (1995) Macrophages in T cell line-mediated, demyelinating, and chronic relapsing experimental autoimmune encephalomyelitis in Lewis rats.
Clin Exp Immunol. 100 (2): 344-51.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
Song, E. et al. (2002) Early application of Met-RANTES ameliorates chronic allograft nephropathy.
Kidney Int. 61: 676-85.
Moreno-Manzano, V. et al. (2003) Retinoids as a potential treatment for experimental puromycin-induced nephrosis
Br J Pharmacol. 139: 823-31.
Shiraishi, H. et al. (2002) Antibody binding to fas ligand attenuates inflammatory cell infiltration and cytokine secretion, leading to reduction of myocardial infarct areas and reperfusion injury.
Lab Invest. 82: 1121-9.
Rusai, K. et al. (2008) Administration of interleukin-1 receptor antagonist ameliorates renal ischemia-reperfusion injury.
Transpl Int. 21: 572-80.
View The Latest Product References
Antus, B. et al. (2001) Contribution of androgens to chronic allograft nephropathy is mediated by dihydrotestosterone
Kidney Int. 60: 1955-63.
Antus, B. et al. (2005) Effects of progesterone and selective oestrogen receptor modulators on chronic allograft nephropathy in rats.
Nephrol Dial Transplant. 20: 329-35.
Kikuchi, H. et al. (2000) Severe proteinuria, sustained for 6 months, induces tubular epithelial cell injury and cell infiltration in rats but not progressive interstitial fibrosis.
Nephrol Dial Transplant.15: 799-810.
Abrams, M.B. et al. (2009) Multipotent mesenchymal stromal cells attenuate chronic inflammation and injury-induced sensitivity to mechanical stimuli in experimental spinal cord injury.
Restor Neurol Neurosci. 27: 307-21.
Robichon, R. et al. (2005) Pig xenografts to the immunocompetent rat brain: Survival rates using distinct neurotoxic lesions in the nigrostriatal pathway and two rat strains.
Exp Neurol. 194: 333-40.
de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168: 5832-9.
Pamukcu, O. et al. (2016) Anti-inflammatory role of obestatin in autoimmune myocarditis.
Clin Exp Pharmacol Physiol. 43 (1): 47-55.
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