Mouse anti Rat CD47 antibody, clone OX-101
recognizes the rat homologue of human CD47, also known as integrin associated protein (IAP). Rat CD47 is a 303 amino acid ӯ33 kDa multi pass transmembrane glycoprotein bearing a single V-type Ig-like
domain and has been identified as the ligand for the rat signal regulatory protein, SIRP (Vernon-Wilson et al. 2000
). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.
Mouse anti Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilson et al. 2000
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Rat thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
integrin-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD47 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD47 antibody
Vernon-Wilson, E.F. et al. (2000) CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1.
Eur J Immunol. 30 (8): 2130-7.
de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168 (11): 5832-9.
Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
Cell Mol Life Sci. 66: 1271-82.
Barrès, C. et al. (2010) Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.
Blood. 115: 696-705.
Kurihara H et al. (2010) SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.
Am J Physiol Renal Physiol. 299 (3): F517-27.