CD47 antibody | OX-101



Mouse anti Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilson et al. 2000).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P97829
- Entrez Gene
- Cd47
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0007229 integrin-mediated signaling pathway
- GO:0016477 cell migration
- Regulatory
- For research purposes only
Applications of CD47 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 | 1/100 | |
Immunoprecipitation | |||
Western Blotting |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Product Specific References
References for CD47 antibody
-
Vernon-Wilson, E.F. et al. (2000) CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1.
Eur J Immunol. 30 (8): 2130-7. -
de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168 (11): 5832-9. -
Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
Cell Mol Life Sci. 66: 1271-82. -
Barrès, C. et al. (2010) Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.
Blood. 115: 696-705. -
Kurihara H et al. (2010) SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.
Am J Physiol Renal Physiol. 299 (3): F517-27.
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