CD47 antibody | OX-101
Mouse anti Rat CD47 antibody, clone OX-101 recognizes the rat homologue of human CD47, also known as integrin associated protein (IAP). Rat CD47 is a 303 amino acid ӯ33 kDa multi pass transmembrane glycoprotein bearing a single V-type Ig-like domain and has been identified as the ligand for the rat signal regulatory protein, SIRP (Vernon-Wilson et al. 2000). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.
Mouse anti Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilson et al. 2000).
Mouse anti Rat CD47 antibody, clone OX-101 has been reported to block the interaction of CD47 and SIRP (Vernon-Wilson et al. 2000).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P97829
- Entrez Gene
- Cd47
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0007229 integrin-mediated signaling pathway
- GO:0016477 cell migration
- Regulatory
- For research purposes only
Applications of CD47 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
References for CD47 antibody
-
Vernon-Wilson, E.F. et al. (2000) CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1.
Eur J Immunol. 30 (8): 2130-7. -
de Vries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168 (11): 5832-9. -
Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
Cell Mol Life Sci. 66: 1271-82. -
Barrès, C. et al. (2010) Galectin-5 is bound onto the surface of rat reticulocyte exosomes and modulates vesicle uptake by macrophages.
Blood. 115: 696-705. -
Kurihara H et al. (2010) SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.
Am J Physiol Renal Physiol. 299 (3): F517-27.
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