CD45RA antibody | OX-33
Mouse anti Rat CD45RA antibody, clone OX-33 (MCA340PE) used for the identification of rat B-cells by flow cytometry.
Image caption:
Effects of a high fat diet (HFD) vs. standard diet (SD) on percentage of splenic total and apoptotic B cells over time. a Density plots depicting gating strategy for B220+ cells in the spleen (left plot) and analysis of early (Annexin V+/7-AAD-) and late (Annexin V+/7-AAD+) apoptotic B cells in the pleen (right plot). b, c Circles represent mean values at different age groups [grey circle: baseline value, closed circles: HFD, open circles: SD]. b percentage of splenic B220+ B cells. c percentage of early apoptotic (left graph) or late apoptotic (right graph) B cells in the spleen. ANOVA was used to determine overall difference between dietary regimen and age groups. Error bars represent the S.E.M. Young: 2 month, old: 18–24 month
From: Pongratz G, Lowin T, Kob R, Buettner R, Bertsch T, Bollheimer LC.
A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
Immun Ageing. 2015 Sep 28;12:12.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD45RA antibody, clone OX-33 (MCA340G) used to evaluate CD45RA expression in rat brain following focal injury by immunofluorescence.
Image caption:
Human bone marrow mesenchymal stem cells (hBM-MSCs) or their extracellular vesicles (EVs) transplanted intra-arterially alleviate CD45RA+ cells in the rat brain evoked by focal brain injury. a Representative images of CD45RA staining in the rat brain of control rats, focal brain injured rats left intact, and those receiving hBM-MSC or EV transplantation. Scale bars 20 μm.
From: Dabrowska S, Andrzejewska A, Strzemecki D, Muraca M, Janowski M, Lukomska B.
Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
J Neuroinflammation. 2019 Nov 13;16(1):216.
doi: 10.1186/s12974-019-1602-5. PubMed PMID: 31722731.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD45RA (B Cells Only)
- Product Type
- Monoclonal Antibody
- Clone
- OX-33
- Isotype
- IgG1
- Specificity
- CD45RA
- Region
- (B CELLS ONLY)
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- MCA340R, MCA340G: Phosphate buffered saline
- MCA340GA: Phosphate buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Purified Rat spleen L-CA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
1/100 | 1/500 |
| Immunohistology - Paraffin 1 | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73.
References for CD45RA antibody
-
McCall, M.N. et al. (1992) Expression of soluble isoforms of rat CD45. Analysis by electron microscopy and use in epitope mapping of anti-CD45R monoclonal antibodies.
Immunology. 76: 310-7. -
Cho, W.S. et al. (2012) NiO and Co3O4 nanoparticles induce lung DTH-like responses and alveolar lipoproteinosis.
Eur Respir J. 39 (3): 546-57. -
Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
Cell Mol Immunol. 9 (2): 163-74. -
Han, X. et al. (2013) Porphyromonas gingivalis infection-associated periodontal bone resorption is dependent on receptor activator of NF-κB ligand.
Infect Immun. 81 (5): 1502-9. -
Marolda, R. et al. (2013) Differential targeting of immune-cells by Pixantrone in experimental myasthenia gravis.
J Neuroimmunol. 258 (1-2): 41-50. -
Okamura, T. et al. (2013) Phenotypic Characterization of LEA Rat: A New Rat Model of Nonobese Type 2 Diabetes.
J Diabetes Res. 2013: 986462. -
Denecke, C. et al. (2013) Synergistic effects of prolonged warm ischemia and donor age on the immune response following donation after cardiac death kidney transplantation.
Surgery. 153 (2): 249-61. -
Stefanski, V. et al. (2013) Differential effect of severe and moderate social stress on blood immune and endocrine measures and susceptibility to collagen type II arthritis in male rats.
Brain Behav Immun. 29: 156-65.
View The Latest Product References
-
Pilatz, A. et al. (2015) Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up.
Andrologia. 47 (2): 160-7. -
Pongratz, G. et al. (2015) A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
Immun Ageing. 12: 12. -
Lu, J.H. et al. (2015) GABAergic neurons in cerebellar interposed nucleus modulate cellular and humoral immunity via hypothalamic and sympathetic pathways.
J Neuroimmunol. 283: 30-8. -
Williamson, L.L. et al. (2016) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.
Brain Behav Immun. 51: 14-28. -
Ogawa, B. et al. (2019) Strain differences in histopathological features of lymphoid tissues of SD and F344 rats in a T cell-dependent antibody response assay of cyclophosphamide.
J Toxicol Pathol. 32 (3): 143-54. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Dabrowska, S. et al. (2019) Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
J Neuroinflammation. 16 (1): 216. -
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines. 10 (5): 1021.
- RRID
- AB_321425
- UniProt
- P04157
- Entrez Gene
- Ptprc
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- View More GO Terms
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
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