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CD45RA antibody | OX-33

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Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 1 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 2 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 3 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 4 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 5 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 6 Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 thumbnail image 7
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 1
Figure A. A647 conjugated mouse anti rat CD45 (MCA43A647) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. A647 conjugated mouse anti rat CD45 (MCA43A647) and FITC conjugated mouse anti rat CD45RA (MCA340FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 2
Figure A. FITC conjugated mouse anti rat CD45RA (MCA340FT) and A647 conjugated mouse IgG1 isotype control (MCA1209A647). Figure B. FITC conjugated mouse anti rat CD45RA (MCA340FT) and A647 conjugated mouse anti rat CD45 (MCA43A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 3
Figure A. RPE conjugated mouse anti rat CD45RA (MCA340PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated mouse anti rat CD45RA (MCA340PE) and FITC conjugated mouse anti rat MHC-II (MCA51FT). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 4
Figure A. FITC conjugated mouse anti rat MHC-II (MCA51FT) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat MHC-II (MCA51FT) and RPE conjugated mouse anti rat CD45RA (MCA340PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 5
Published customer image:
Mouse anti Rat CD45RA antibody, clone OX-33 (MCA340PE) used for the identification of rat B-cells by flow cytometry.
Image caption:
Effects of a high fat diet (HFD) vs. standard diet (SD) on percentage of splenic total and apoptotic B cells over time. a Density plots depicting gating strategy for B220+ cells in the spleen (left plot) and analysis of early (Annexin V+/7-AAD-) and late (Annexin V+/7-AAD+) apoptotic B cells in the pleen (right plot). b, c Circles represent mean values at different age groups [grey circle: baseline value, closed circles: HFD, open circles: SD]. b percentage of splenic B220+ B cells. c percentage of early apoptotic (left graph) or late apoptotic (right graph) B cells in the spleen. ANOVA was used to determine overall difference between dietary regimen and age groups. Error bars represent the S.E.M. Young: 2 month, old: 18–24 month

From: Pongratz G, Lowin T, Kob R, Buettner R, Bertsch T, Bollheimer LC.
A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
Immun Ageing. 2015 Sep 28;12:12.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 6
Published customer image:
Mouse anti Rat CD45RA antibody, clone OX-33 (MCA340G) used to evaluate CD45RA expression in rat brain following focal injury by immunofluorescence.
Image caption:
Human bone marrow mesenchymal stem cells (hBM-MSCs) or their extracellular vesicles (EVs) transplanted intra-arterially alleviate CD45RA+ cells in the rat brain evoked by focal brain injury. a Representative images of CD45RA staining in the rat brain of control rats, focal brain injured rats left intact, and those receiving hBM-MSC or EV transplantation. Scale bars 20 μm.

From: Dabrowska S, Andrzejewska A, Strzemecki D, Muraca M, Janowski M, Lukomska B.
Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
J Neuroinflammation. 2019 Nov 13;16(1):216.
doi: 10.1186/s12974-019-1602-5. PubMed PMID: 31722731.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat CD45RA (B Cells Only) Antibody, clone OX-33 gallery image 7
Formalin fixed, paraffin embedded rat small intestinal mucosal associated lymphoid tissue (MALT) stained with Mouse Anti-Rat CD45RA (MCA340G), biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.

Mouse anti Rat CD45RA:FITC

Mouse anti Rat CD45RA (B Cells Only)

Mouse anti Rat CD45RA:RPE

Product Type
Monoclonal Antibody
Clone
OX-33
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA340FT
Datasheet
F
SDS
0.1 mg loader

MCA340G
Datasheet
C F P*
SDS
1 mg loader

MCA340R
Datasheet
C F P*
SDS
0.25 mg loader

MCA340GA
Datasheet
C F P*
SDS
0.1 mg loader

MCA340PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes (Barclay et al. 1987).

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Purified Rat spleen L-CA
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P04157
Entrez Gene
Ptprc
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0019887 protein kinase regulator activity
GO:0009898 internal side of plasma membrane
GO:0010332 response to gamma radiation
GO:0019901 protein kinase binding
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD45RA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 1
Flow Cytometry Neat 1/10
  1. 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
This antibody does not always give a clear separation between positives and negatives in some cell preparations. If users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent MCA340PE which generally exhibits a peak of higher mean fluorescence.

Use 10ul of the suggested working dilutions to label 106 cells in 100ul.

Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD45RA antibody

  1. Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
    Eur J Immunol. 15 (2): 168-73.
  2. Barclay, A.N. et al. (1987) Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus.
    EMBO J. 6:1259-1264.
  3. Barclay, A.N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42:593-600.
  4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  5. McCall, M.N. et al. (1992) Expression of soluble isoforms of rat CD45. Analysis by electron microscopy and use in epitope mapping of anti-CD45R monoclonal antibodies.
    Immunology. 76: 310-7.
  6. Cho, W.S. et al. (2012) NiO and Co3O4 nanoparticles induce lung DTH-like responses and alveolar lipoproteinosis.
    Eur Respir J. 39 (3): 546-57.
  7. Williamson Lauren L. et al. (2015) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection
    Brain, Behavior, and Immunity. pii: S0889-1591(15)00240-8.
  8. Pilatz, A. et al. (2015) Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up.
    Andrologia. 47 (2): 160-7.
  9. Stefanski, V. et al. (2013) Differential effect of severe and moderate social stress on blood immune and endocrine measures and susceptibility to collagen type II arthritis in male rats.
    Brain Behav Immun. 29: 156-65.
  10. Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
    Cell Mol Immunol. 9 (2): 163-74.
  11. Okamura, T. et al. (2013) Phenotypic Characterization of LEA Rat: A New Rat Model of Nonobese Type 2 Diabetes.
    J Diabetes Res. 2013: 986462.
  12. Denecke, C. et al. (2013) Synergistic effects of prolonged warm ischemia and donor age on the immune response following donation after cardiac death kidney transplantation.
    Surgery. 153 (2): 249-61.
  13. Marolda, R. et al. (2013) Differential targeting of immune-cells by Pixantrone in experimental myasthenia gravis.
    J Neuroimmunol. 258 (1-2): 41-50.
  14. Han, X. et al. (2013) Porphyromonas gingivalis infection-associated periodontal bone resorption is dependent on receptor activator of NF-κB ligand.
    Infect Immun. 81 (5): 1502-9.
  15. Pongratz, G. et al. (2015) A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
    Immun Ageing. 12: 12.
  16. Lu, J.H. et al. (2015) GABAergic neurons in cerebellar interposed nucleus modulate cellular and humoral immunity via hypothalamic and sympathetic pathways.
    J Neuroimmunol. 283: 30-8.
  17. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  18. Dabrowska, S. et al. (2019) Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
    J Neuroinflammation. 16 (1): 216.
  19. Ogawa, B. et al. (2019) Strain differences in histopathological features of lymphoid tissues of SD and F344 rats in a T cell-dependent antibody response assay of cyclophosphamide.
    J Toxicol Pathol. 32 (3): 143-54.

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