CD45RA antibody | OX-33
Mouse anti Rat CD45RA antibody, clone OX-33 (MCA340PE) used for the identification of rat B-cells by flow cytometry.
Image caption:
Effects of a high fat diet (HFD) vs. standard diet (SD) on percentage of splenic total and apoptotic B cells over time. a Density plots depicting gating strategy for B220+ cells in the spleen (left plot) and analysis of early (Annexin V+/7-AAD-) and late (Annexin V+/7-AAD+) apoptotic B cells in the pleen (right plot). b, c Circles represent mean values at different age groups [grey circle: baseline value, closed circles: HFD, open circles: SD]. b percentage of splenic B220+ B cells. c percentage of early apoptotic (left graph) or late apoptotic (right graph) B cells in the spleen. ANOVA was used to determine overall difference between dietary regimen and age groups. Error bars represent the S.E.M. Young: 2 month, old: 18–24 month
From: Pongratz G, Lowin T, Kob R, Buettner R, Bertsch T, Bollheimer LC.
A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
Immun Ageing. 2015 Sep 28;12:12.
Mouse anti Rat CD45RA:FITC
Mouse anti Rat CD45RA (B Cells Only)
Mouse anti Rat CD45RA:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-33
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA340FT | F | 0.1 mg | ||||
| MCA340G | C F P* | 1 mg | ||||
| MCA340GA | C F P* | 0.1 mg | ||||
| MCA340R | C F P* | 0.25 mg | ||||
| MCA340PE | F | 100 Tests |
Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (Barclay et al. 1987).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Purified Rat spleen L-CA
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of reconstitution
More Information
- UniProt
- P04157 Related reagents
- Entrez Gene
- Ptprc Related reagents
- GO Terms
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0019887 protein kinase regulator activity
- GO:0009898 internal side of plasma membrane
- GO:0010332 response to gamma radiation
- GO:0019901 protein kinase binding
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
- Regulatory
- For research purposes only
Applications of CD45RA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunohistology - Frozen | 1/100 | 1/500 | |
| Immunohistology - Paraffin 1 | |||
| Flow Cytometry | Neat | 1/10 |
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- This antibody does not always give a clear separation between positives and negatives in some cell preparations. If users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent MCA340PE which generally exhibits a peak of higher mean fluorescence.
Use 10ul of the suggested working dilutions to label 106 cells in 100ul. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:RPE | MCA1209PE | 100 Tests | F |
Product Specific References
References for CD45RA antibody
-
Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73. -
Barclay, A.N. et al. (1987) Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus.
EMBO J. 6:1259-1264. -
Barclay, A.N. et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42:593-600. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
McCall, M.N. et al. (1992) Expression of soluble isoforms of rat CD45. Analysis by electron microscopy and use in epitope mapping of anti-CD45R monoclonal antibodies.
Immunology. 76: 310-7. -
Cho, W.S. et al. (2012) NiO and Co3O4 nanoparticles induce lung DTH-like responses and alveolar lipoproteinosis.
Eur Respir J. 39 (3): 546-57. -
Williamson Lauren L. et al. (2015) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection
Brain, Behavior, and Immunity. pii: S0889-1591(15)00240-8. -
Pilatz, A. et al. (2015) Experimental Escherichia coli epididymitis in rats: assessment of testicular involvement in a long-term follow-up.
Andrologia. 47 (2): 160-7. -
Stefanski, V. et al. (2013) Differential effect of severe and moderate social stress on blood immune and endocrine measures and susceptibility to collagen type II arthritis in male rats.
Brain Behav Immun. 29: 156-65. -
Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
Cell Mol Immunol. 9 (2): 163-74. -
Okamura, T. et al. (2013) Phenotypic Characterization of LEA Rat: A New Rat Model of Nonobese Type 2 Diabetes.
J Diabetes Res. 2013: 986462. -
Denecke, C. et al. (2013) Synergistic effects of prolonged warm ischemia and donor age on the immune response following donation after cardiac death kidney transplantation.
Surgery. 153 (2): 249-61. -
Marolda, R. et al. (2013) Differential targeting of immune-cells by Pixantrone in experimental myasthenia gravis.
J Neuroimmunol. 258 (1-2): 41-50. -
Han, X. et al. (2013) Porphyromonas gingivalis infection-associated periodontal bone resorption is dependent on receptor activator of NF-κB ligand.
Infect Immun. 81 (5): 1502-9. -
Pongratz, G. et al. (2015) A sustained high fat diet for two years decreases IgM and IL-1 beta in ageing Wistar rats.
Immun Ageing. 12: 12. -
Lu, J.H. et al. (2015) GABAergic neurons in cerebellar interposed nucleus modulate cellular and humoral immunity via hypothalamic and sympathetic pathways.
J Neuroimmunol. 283: 30-8.
