CD44 antibody | OX-50

Mouse anti Rat CD44

Product Type
Monoclonal Antibody
Clone
OX-50
Isotype
IgG1
Specificity
CD44

Product Code Applications Pack Size List Price Your Price Qty
MCA643GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.1 mg loader
List Price Your Price
loader
MCA643R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD44 antibody, clone OX-50 recognizes the rat CD44 cell surface antigen, also known as Extracellular matrix receptor III, P90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1 or Phagocytic glycoprotein I.

CD44 is a 482 amino acid ~85 kDa single pass type I transmembrane glycoprotein, expressed by T cells, B cells, macrophages and thymocytes, with expression being increased following activation.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat T cell blasts.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0/1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/50 Pack Size: 0.1 mg
1/100 Pack Size: 0.25 mg
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD44 antibody

  1. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18:1389-98.
  2. Jiang, T.S. et al. (2010) Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats.
    Mol Vis. 16: 1304-16.
  3. Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
    Nephrol Dial Transplant. 25: 2898-908.
  4. Li, S. et al. (2010) Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
    Neurosci Res. 67: 293-9.
  5. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  6. Rice, C.M. et al. (2010) Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells.
    Br J Haematol. 148: 441-4.
  7. Carulli, D. et al (2006) Composition of perineuronal nets in the adult rat cerebellum and the cellular origin of their components.
    J Comp Neurol. 494: 559-77.
  8. Galtrey, C.M. et al. (2008) Distribution and synthesis of extracellular matrix proteoglycans, hyaluronan, link proteins and tenascin-R in the rat spinal cord.
    Eur J Neurosci. 27: 1373-90.
  9. View The Latest Product References
  10. Hofmann, N. et al. (2002) Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.
    BMC Immunol. 3: 11.
  11. Walther, M. et al. (2001) Exogenous antigen containing perivascular phagocytes induce a non-encephalitogenic extravasation of primed lymphocytes.
    J Neuroimmunol. 117: 30-42.
  12. Suzuki, A. et al. (2006) Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint.
    Anat Rec A Discov Mol Cell Evol Biol. 288: 646-52.
  13. Goransson, V. et al. (2004) Renal hyaluronan accumulation and hyaluronan synthase expression after ischaemia-reperfusion injury in the rat.
    Nephrol Dial Transplant.19: 823-30.
  14. Campbell, N.G. et al. (2016) Cell Size Critically Determines Initial Retention of Bone Marrow Mononuclear Cells in the Heart after Intracoronary Injection: Evidence from a Rat Model.
    PLoS One. 11 (7): e0158232.
  15. Bejar, M.T. et al. (2016) Inhibition of Notch rescues the angiogenic potential impaired by cardiovascular risk factors in epicardial adipose stem cells.
    FASEB J. 30 (8): 2849-59.
  16. Rochefort, G.Y. et al. (2006) Multipotential mesenchymal stem cells are mobilized into peripheral blood by hypoxia.
    Stem Cells. 24 (10): 2202-8.
  17. Redondo, J. et al. (2015) Reductions in kinesin expression are associated with nitric oxide-induced axonal damage.
    J Neurosci Res. 93 (6): 882-92.
  18. Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
    Contrast Media Mol Imaging. 2019: 5184105.
  19. Aminzadeh, A. et al. (2020) Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
    Cell J. 22 (Suppl 1): 38-48.
  20. Paiva, R.G. et al. (2020) Stem cells in end-to-side neurorrhaphy. Experimental study in rats
    Acta Cirúrgica Brasileira. 35 (12) [Epub ahead of print].
  21. Hou, B. et al. (2018) Comparison of the Effects of BMSC-derived Schwann Cells and Autologous Schwann Cells on Remyelination Using a Rat Sciatic Nerve Defect Model.
    Int J Biol Sci. 14 (13): 1910-22.
  22. Porwal, K. et al.kfz172 (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
    Toxicol Sci. kfz172.
  23. Chang, H.H. et al. (2019) Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy.
    Antioxidants (Basel). 9 (1): 2.
  24. Wu, J. et al. (2022) Reinforcing the function of bone graft via the Ca-P ceramics dynamic behavior-enhanced osteogenic microenvironment for optimal bone regeneration and reconstruction
    Applied Materials Today. 27: 101465.

Synonyms
H-CAM
PGP-1
RRID
AB_566756
UniProt
P26051
Entrez Gene
Cd44
GO Terms
GO:0001558 regulation of cell growth
GO:0001955 blood vessel maturation
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
GO:0005634 nucleus
GO:0006954 inflammatory response
GO:0014070 response to organic cyclic compound
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016477 cell migration
GO:0019901 protein kinase binding
GO:0031175 neuron projection development
GO:0033031 positive regulation of neutrophil apoptosis
GO:0033189 response to vitamin A
GO:0034238 macrophage fusion
GO:0043234 protein complex
GO:0051219 phosphoprotein binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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