CD25 antibody | OX-39

Product Code Applications Pack Size List Price Your Price Qty
MCA273GA
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SDS Safety Datasheet SDS
C E F IP P * 0.1 mg loader
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MCA273R
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C E F IP P * 0.25 mg loader
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Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.

Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).

Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Stimulated Rat T cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0/1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
  1. 1PLP fixation is recommended, see Whiteland. J.L. et al.(1995)for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD25 antibody

  1. Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
    Mol Immunol. 24 (12): 1281-90.
  2. Charteris DG & Lightman SL (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
    Immunology. 78 (3): 387-92.
  3. Hayosh, N.S. & Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors.
    J Immunol. 138 (11): 3771-5.
  4. Tellides, G. et al. (1987) Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy.
    Transplant Proc. 19 (5): 4231-3.
  5. Signore, A. et al. (1987) Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes.
    Lancet. 2 (8558): 537-40.
  6. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  7. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  8. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87 (12): 1515-22.
  9. View The Latest Product References
  10. Fujiki, M. et al. (2010) Induced tolerance to rat liver allografts involves the apoptosis of intragraft T cells and the generation of CD4(+)CD25(+)FoxP3(+) T regulatory cells.
    Liver Transpl. 16: 147-54.
  11. Ghiringhelli, F. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.
    J Exp Med. 202: 919-29.
  12. Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
    J Autoimmun. 67: 57-64.
  13. Lühder, F. et al. (2017) Laquinimod enhances central nervous system barrier functions.
    Neurobiol Dis. 102: 60-9.
  14. Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
    Int J Clin Exp Pathol. 10(5): 5252-62.
  15. Timrott, K. et al. (2020) The importance of MHC class II in allogeneic bone marrow transplantation and chimerism-based solid organ tolerance in a rat model.
    PLoS One. 15 (5): e0233497.
  16. Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
    Polymers (Basel). 13 (16): 2684.
  17. Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
    Cell Tissue Res. 386 (3): 617-36.
  18. Zakerkish, F. et al. (2021) Differential effects of the immunosuppressive calcineurin inhibitors cyclosporine-A and tacrolimus on ovulation in a murine model.
    Hum Reprod Open. 2021 (2): hoab012.

Flow Cytometry

Immunohistology - Paraffin

Synonyms
IL-2R Alpha Chain
RRID
AB_324114
UniProt
P26897
Entrez Gene
Il2ra
GO Terms
GO:0002437 inflammatory response to antigenic stimulus
GO:0016021 integral to membrane
GO:0004911 interleukin-2 receptor activity
GO:0008144 drug binding
GO:0045582 positive regulation of T cell differentiation
GO:0050687 negative regulation of defense response to virus
GO:0050777 negative regulation of immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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