CD25 antibody | OX-39
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Mouse anti Rat CD25
- Product Type
- Monoclonal Antibody
|Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.
Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Stimulated Rat T cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS0/1 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
- 1PLP fixation is recommended, see Whiteland. J.L. et al.(1995)for details.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG1 Negative Control||MCA1209||F||0.1 mg||Log in|
|List Price||Your Price|
|Description||Mouse IgG1 Negative Control|
References for CD25 antibody
Paterson, D.J. et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.
Mol Immunol. 24 (12): 1281-90.
Charteris DG & Lightman SL (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis.
Immunology. 78 (3): 387-92.
Hayosh, N.S. & Swanborg, R.H. (1987) Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors.
J Immunol. 138 (11): 3771-5.
Tellides, G. et al. (1987) Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy.
Transplant Proc. 19 (5): 4231-3.
Signore, A. et al. (1987) Detection of activated lymphocytes in endocrine pancreas of BB/W rats by injection of 123I-interleukin-2: an early sign of type 1 diabetes.
Lancet. 2 (8558): 537-40.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35.
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87 (12): 1515-22.
View The Latest Product References
Fujiki, M. et al. (2010) Induced tolerance to rat liver allografts involves the apoptosis of intragraft T cells and the generation of CD4(+)CD25(+)FoxP3(+) T regulatory cells.
Liver Transpl. 16: 147-54.
Ghiringhelli, F. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.
J Exp Med. 202: 919-29.
Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
J Autoimmun. 67: 57-64.
Lühder, F. et al. (2017) Laquinimod enhances central nervous system barrier functions.
Neurobiol Dis. 102: 60-9.
Sun, J. et al. (2017) Pentapeptide PLNPK ameliorates adjuvant arthritis and inhibits T cell activation by suppressing Lck and PI3K activities
Int J Clin Exp Pathol. 10(5): 5252-62.
Timrott, K. et al. (2020) The importance of MHC class II in allogeneic bone marrow transplantation and chimerism-based solid organ tolerance in a rat model.
PLoS One. 15 (5): e0233497.
Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
Polymers (Basel). 13 (16): 2684.
Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
Cell Tissue Res. 386 (3): 617-36.
Zakerkish, F. et al. (2021) Differential effects of the immunosuppressive calcineurin inhibitors cyclosporine-A and tacrolimus on ovulation in a murine model.
Hum Reprod Open. 2021 (2): hoab012.
Zhou, X. et al. (2022) Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.
Nat Commun. 13 (1): 6672.
- IL-2R Alpha Chain
- Entrez Gene
- GO Terms
- GO:0002437 inflammatory response to antigenic stimulus
- GO:0016021 integral to membrane
- GO:0004911 interleukin-2 receptor activity
- GO:0008144 drug binding
- GO:0045582 positive regulation of T cell differentiation
- GO:0050687 negative regulation of defense response to virus
- GO:0050777 negative regulation of immune response
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