CD172a antibody | ED9

Mouse anti Rat CD172a

Product Type
Monoclonal Antibody
Clone
ED9
Isotype
IgG1
Specificity
CD172a

Product Code Applications Pack Size List Price Your Price Qty
MCA620R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP WB 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRPα -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Spleen cell homogenate.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1 1/100 1/1000
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD172a antibody

  1. Damoiseaux, J.G. et al. (1989) Rat bone marrow and monocyte cultures: influence of culture time and lymphokines on the expression of macrophage differentiation antigens.
    J Leukoc Biol. 46 (3): 246-53.
  2. Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
    J Leukoc Biol. 46 (6): 556-64.
  3. Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
    J Immunol. 161 (4): 1853-9.
  4. DeVries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
    J Immunol. 168 (11): 5832-9.
  5. Blackbeard, J. et al. (2007) Quantification of the rat spinal microglial response to peripheral nerve injury as revealed by immunohistochemical image analysis and flow cytometry.
    J Neurosci Methods. 164 (2): 207-17.
  6. Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
    Immunology. 123 (4): 480-90.
  7. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  8. Holler, J. et al. (2008) Neuropeptide Y is expressed by rat mononuclear blood leukocytes and strongly down-regulated during inflammation.
    J Immunol. 181 (10): 6906-12.
  9. View The Latest Product References
  10. Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
    Cell Tissue Res. Oct 04 [Epub ahead of print].

Synonyms
SIRP ALPHA
RRID
AB_323093
UniProt
P97710
Entrez Gene
Sirpa
GO Terms
GO:0016477 cell migration
GO:0017124 SH3 domain binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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