CD163 antibody | ED2
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| Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).
Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
Our CD163 (ED2) Antibody has been referenced in >116 publications*
*Based on June 2020 data from CiteAb's antibody search engine.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat Spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/100|
|Immunohistology - Paraffin 1|
- 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Histology Positive Control Tissue
References for CD163 antibody
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Harty, M.W. et al. (2010) Neutrophil depletion blocks early collagen degradation in repairing cholestatic rat livers.
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Loureiro, J. et al. (2010) BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposure.
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Muller, D.N. et al. (2002) Immunosuppressive treatment protects against angiotensin II-induced renal damage.
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Rosas-Ballina, M. et al. (2008) Splenic nerve is required for cholinergic antiinflammatory pathway control of TNF in endotoxemia.
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Wehner, S. et al. (2007) Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents.
Gut. 56: 176-85.
Yu, Y. et al. (2010) Brain perivascular macrophages and the sympathetic response to inflammation in rats after myocardial infarction.
Hypertension. 55: 652-9.
View The Latest Product References
Zeng, Z. et al. (2010) Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: the role of Kupffer cells.
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Teng, B.T. et al. (2011) Protective effect of caspase inhibition on compression-induced muscle damage.
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Santos, M. et al. (2009) An unbiased stereological study on subpopulations of rat liver macrophages and on their numerical relation with the hepatocytes and stellate cells.
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Yamato, M. et al. (2009) PET and macro- and microautoradiographic studies combined with immunohistochemistry for monitoring rat intestinal ulceration and healing processes.
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Jensen, K.T. et al. (2008) Early inflammatory response of knee ligaments to prolotherapy in a rat model.
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Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
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Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation.
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Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
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Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
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Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
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Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.
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