CD163 antibody | ED2

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MCA342GA
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SDS Safety Datasheet SDS
C F IF IP P * WB 0.1 mg loader
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MCA342R
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SDS Safety Datasheet SDS
C F IF IP P * WB 0.25 mg loader
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Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).

Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).

Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
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Our CD163 (ED2) Antibody has been referenced in >116 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat Spleen cell homogenate.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Histology Positive Control Tissue
Liver

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References for CD163 antibody

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    J Exp Med. 202: 919-29.
  2. Hamada, T. et al. (2007) Oncostatin M gene therapy attenuates liver damage induced by dimethylnitrosamine in rats.
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  3. Harty, M.W. et al. (2010) Neutrophil depletion blocks early collagen degradation in repairing cholestatic rat livers.
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  4. Loureiro, J. et al. (2010) BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposure.
    Nephrol Dial Transplant. 25: 1098-108.
  5. Muller, D.N. et al. (2002) Immunosuppressive treatment protects against angiotensin II-induced renal damage.
    Am J Pathol. 161: 1679-93.
  6. Rosas-Ballina, M. et al. (2008) Splenic nerve is required for cholinergic antiinflammatory pathway control of TNF in endotoxemia.
    Proc Natl Acad Sci U S A. 105: 11008-13.
  7. Wehner, S. et al. (2007) Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents.
    Gut. 56: 176-85.
  8. Yu, Y. et al. (2010) Brain perivascular macrophages and the sympathetic response to inflammation in rats after myocardial infarction.
    Hypertension. 55: 652-9.
  9. View The Latest Product References
  10. Zeng, Z. et al. (2010) Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: the role of Kupffer cells.
    World J Gastroenterol. 16: 1285-92.
  11. Teng, B.T. et al. (2011) Protective effect of caspase inhibition on compression-induced muscle damage.
    J Physiol. 589: 3349-69.
  12. Dessem, D. et al. (2010) Eccentric muscle contraction and stretching evoke mechanical hyperalgesia and modulate CGRP and P2X(3) expression in a functionally relevant manner.
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  13. Santos, M. et al. (2009) An unbiased stereological study on subpopulations of rat liver macrophages and on their numerical relation with the hepatocytes and stellate cells.
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  14. Yamato, M. et al. (2009) PET and macro- and microautoradiographic studies combined with immunohistochemistry for monitoring rat intestinal ulceration and healing processes.
    J Nucl Med. 50: 266-73.
  15. Starke-Buzetti, W.A. et al. (2008) Increased glial-derived neurotrophic factor in the small intestine of rats infected with the tapeworm, Hymenolepis diminuta.
    Int J Exp Pathol. 89: 458-65.
  16. Jensen, K.T. et al. (2008) Early inflammatory response of knee ligaments to prolotherapy in a rat model.
    J Orthop Res. 26: 816-23.
  17. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
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  18. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation.
    Transplant Proc. 3: 3166-70.
  19. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
    Immunobiol. 182: 88-99.
  20. Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
    Prog Histochem Cytochem. 27 (2): 1-65.
  21. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  22. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.
    PhD Thesis. Vrije University, Amsterdam.
  23. Deng, X. et al. (2005) Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats.
    Am J Pathol. 166 (1): 93-106.
  24. Piquet-Pellorce, I. et al. (2005) Identification of the leukemia inhibitory factor cell targets within the rat testis.
    Biol Reprod. 72: 602-11.
  25. Fujita, E. et al. (2010) Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages.
    Am J Pathol. 177 (3): 1143-54.
  26. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  27. Baker, S.C. et al. (2011) Cellular integration and vascularisation promoted by a resorbable, particulate-leached, cross-linked poly(ε-caprolactone) scaffold.
    Macromol Biosci. 11 (5): 618-27.
  28. Bedi, A. et al. (2010) Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction.
    J Bone Joint Surg Am. 92: 2387-401.
  29. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87: 1515-22.
  30. Moghaddami, M. et al. (2005) MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium.
    Int Immunol. 17: 1117-30.
  31. Wojcik, M. et al. (2012) Immunodetection of cyclooxygenase-2 (COX-2) is restricted to tissue macrophages in normal rat liver and to recruited mononuclear phagocytes in liver injury and cholangiocarcinoma.
    Histochem Cell Biol. 137: 217-33.
  32. Kawakami, A.P. et al. (2011) Inflammatory Process Modulation by Homeopathic Arnica montana 6CH: The Role of Individual Variation.
    Evid Based Complement Alternat Med. 2011: 917541.
  33. Kajita, M. et al. (2011) iNOS expression in vascular resident macrophages contributes to circulatory dysfunction of splanchnic vascular smooth muscle contractions in portal hypertensive rats.
    Am J Physiol Heart Circ Physiol. 300: H1021-31.
  34. Jiao, K. et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
    PLoS One. 8(1):e53312.
  35. Fujii, Y. et al. (2013) Effect of enzymatically modified isoquercitrin on preneoplastic liver cell lesions induced by thioacetamide promotion in a two-stage hepatocarcinogenesis model using rats.
    Toxicology. 305: 30-40.
  36. Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
    PLoS One. 8: e57406.
  37. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
    Blood. 109 (12): 5223-9.
  38. Park, E.S. et al. (2014) Establishment of a rat model for canine necrotizing meningoencephalitis (NME).
    Vet Pathol. 51 (6): 1151-64.
  39. Keitel, V. et al. (2008) Expression and function of the bile acid receptor TGR5 in Kupffer cells.
    Biochem Biophys Res Commun. 372: 78-84.
  40. Hozumi, Y. et al. (2015) Expression and localization of the diacylglycerol kinase family and of phosphoinositide signaling molecules in adrenal gland.
    Cell Tissue Res. 362 (2): 295-305.
  41. Fernandez-Bustamante, A. et al. (2015) Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
    PLoS One. 10 (7): e0130764.
  42. Adamo, H.H. et al. (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
    PLoS One. 10 (11): e0141601.
  43. Santos, L. et al. (2016) In vitro and in vivo assessment of magnetically actuated biomaterials and prospects in tendon healing.
    Nanomedicine (Lond). 11 (9): 1107-22.
  44. Ibarra V et al. (2016) Evaluation of the Tissue Response to Alginate Encapsulated Islets in an Omentum Pouch Model.
    J Biomed Mater Res A. May 3. [Epub ahead of print]
  45. Tentillier N et al. (2016) Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model.
    J Neurosci. 36 (36): 9375-90.
  46. Zakrzewicz, A. et al. (2015) Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury.
    Mediators Inflamm. 2015: 429653.
  47. Rave-Fränk M et al. (2013) Rat model of fractionated (2 Gy/day) 60 Gy irradiation of the liver: long-term effects.
    Radiat Environ Biophys. 52 (3): 321-38.
  48. Wang, M. et al. (2017) Characterization of the Micro-Environment of the Testis that Shapes the Phenotype and Function of Testicular Macrophages.
    J Immunol. May 1. pii: 1700162. [Epub ahead of print]
  49. Stavenuiter, A.W. et al. (2015) Protective Effects of Paricalcitol on Peritoneal Remodeling during Peritoneal Dialysis.
    Biomed Res Int. 2015: 468574.
  50. Hawkins, K.E. et al. (2017) Targeting resolution of neuroinflammation after ischemic stroke with a lipoxin A4 analog: Protective mechanisms and long-term effects on neurological recovery.
    Brain Behav. 7 (5): e00688.
  51. Almahrog, A.J. et al. (2016) In vivo. association of immunophenotyped macrophages expressing CD163 with PDGF-β in gingival overgrowth-induced by three different categories of medications.
    J Oral Biol Craniofac Res. 6 (1): 10-7.
  52. Han, T.T. et al. (2015) Adipose-derived stromal cells mediate in vivo adipogenesis, angiogenesis and inflammation in decellularized adipose tissue bioscaffolds.
    Biomaterials. 72: 125-37.
  53. Pannell, M. et al. (2016) Adoptive transfer of M2 macrophages reduces neuropathic pain via opioid peptides.
    J Neuroinflammation. 13 (1): 262.
  54. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  55. Bloomer, S.A. et al. (2019) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride
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  56. Azam, M. et al. (2019) Addition of 2-deoxy-d-ribose to clinically used alginate dressings stimulates angiogenesis and accelerates wound healing in diabetic rats.
    J Biomater Appl. 34 (4): 463-475.
  57. Bhandari, S. et al. (2020) Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages.
    BMC Mol Cell Biol. 21 (1): 85.
  58. Solár, P. et al. (2020) Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus.
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  59. Nishida, Y. et al. (2020) Intra-Articular Injection of Stromal Cell-Derived Factor 1α Promotes Meniscal Healing via Macrophage and Mesenchymal Stem Cell Accumulation in a Rat Meniscal Defect Model.
    Int J Mol Sci. 21(15):5454.
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    Histochem Cell Biol. 153 (1): 37-48.
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    J Vet Med Sci. Jan 20 [Epub ahead of print].
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    Neuropathol Appl Neurobiol. 46 (2): 142-159.
  63. Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
    Muscle Nerve. 48 (3): 403-14.
  64. Li, R. et al. (2020) Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization.
    Arthritis Res Ther. 22 (1): 75.
  65. Matsumoto, K. et al. (2021) Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis.
    J Pharmacol Sci. 146 (3): 125-35.
  66. Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
    Polymers (Basel). 13(16):2684.
  67. Kim, S. & Son, Y. (2021) Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro. through Crosstalk between Astrocytes and Microglia.
    Int J Mol Sci. 22 (16): 8800.
  68. Takei, R. et al. (2021) Dynamic switch of immunity and antitumor effects of metformin in rat spontaneous esophageal carcinogenesis.
    Cancer Immunol Immunother. Aug 16 [Epub ahead of print].
  69. Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
    J Vet Med Sci. 83 (9): 1435-42.
  70. Garcia, G.E. et al. (2021) A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
    Cells. 10(8): 2113.
  71. Zakerkish, F. et al. (2021) Differential effects of the immunosuppressive calcineurin inhibitors cyclosporine-A and tacrolimus on ovulation in a murine model.
    Hum Reprod Open. 2021 (2): hoab012.
  72. Anderson, L.E. et al. (2021) Injection of Micronized Human Amnion/Chorion Membrane Results in Increased Early Supraspinatus Muscle Regeneration in a Chronic Model of Rotator Cuff Tear.
    Ann Biomed Eng. Nov 11 [Epub ahead of print].
  73. Hou, Y. et al. (2021) Pseudoginsenoside-F11 promotes functional recovery after transient cerebral ischemia by regulating the microglia/macrophage polarization in rats.
    Int Immunopharmacol. 99: 107896.
  74. Köhler, R. et al. (2022) Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model.
    J Biomed Mater Res A. 110 (1): 52-63.
  75. Pervin, M. et al. (2022) Possible Cytoprotection of Low Dose Lipopolysaccharide in Rat Thioacetamide-Induced Liver Lesions, Focusing on the Analyses of Hepatic Macrophages and Autophagy.
    Toxicol Pathol. : 1926233221076758.
  76. Hoff, U. et al. (2022) The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
    PLoS One. 17 (4): e0266319.
  77. Itoh, M. et al. (2022) Time-series biological responses toward decellularized bovine tendon graft and autograft for 52 consecutive weeks after rat anterior cruciate ligament reconstruction.
    Sci Rep. 12 (1): 6751.

Flow Cytometry

Functional Assays

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
ED2
RRID
AB_321966
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