CD163 antibody | ED2
Mouse anti Rat CD163, clone ED2 (MCA342R) used for the demonstration of infiltrating macrophages in corneal graft tissue by immunohistochemistry on acetone fixed cryosections.
Image caption:
Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01). F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by immunofluorescence microscopy and flow cytometry.
Image caption:
Increase in CD163+ cells with enhanced phagocytic activity in experimentally-induced arthritic cartilage of rat TMJs. A: Flow cytometry analysis and comparison of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within isolated type II collagen expressing (Col-II+) cells from TMJ cartilage from the 8-week experimental group and their age-matched controls. B: Confocal microscope images of the CD163+ cells and assessment of their phagocytic activity in primary cells isolated from TMJ cartilage. The images reveal an increase in CD163+ cells and enhanced co-localization with the FITC-labeled cell debris in 8-week experimental group compared with the age-matched controls. C: Serial confocal images (1–4) of the primary cells isolated from TMJ cartilage of 3-week old rats co-cultured with DiO-labeled cellular debris. Sections were stained with a CD163 antibody and a Cy3-conjugated secondary antibody. Note that the CD163+ cells showed membrane staining (red) and the cell debris (green) was located inside the cell membrane. D: Dynamic observation of the phagocytic process involving living CD163+ cells sorted from TMJ cartilage engulfing cellular debris. Note that the DiO-labeled debris is undergoing phagocytosis by the CD163+ cell indicated within the white box. E: Comparison of the nitric oxide (NO) concentration and amount of intracellular reactive oxygen species (ROS) in the primary cells isolated from TMJ cartilage from 8-week experimental group and their age-matched controls. *P<0.05.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraqffin embedded tissue sections and and flow cytometry on isolated cells.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by flow cytometry.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by flow cytometry on isolated cells.
Image caption:
Exogenous TNF-α increased CD163 expression in primary chondrocytes from TMJ cartilage of 3-week old rats. A: The primary cells isolated from TMJ cartilage of 3-week old rats were positive for type II collagen (Col-II) and aggrecan, as detected by immunofluorescence and toluidine blue, respectively (400× magnification). B: A time-course of induction of CD163 mRNA expression in primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α. C–D: Flow cytometric analysis and graphical representation of the percentage of CD163+ cells within the primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunofluorescence microscopy on isolated cells.
Image caption:
TNF-α increased the phagocytic and migratory activities of CD163+ cells isolated from rat TMJ cartilage. A–B: Confocal microscope images (A) and graphical representation (B) of the numbers of CD163+ cells and their phagocytic activity within primary cells isolated from TMJ cartilage and treated with vehicle, TNF-α alone, or TNF-α and a CD163 neutralizing antibody. The co-localization of the CD163+ cell with DiO-labeled cell debris (arrows), indicates that the cell debris is undergoing phagocytosis by the CD163+ cells, as shown in the insets. Bar: 50 μm. C: Transwell assay combined with immunohistochemical staining of CD163 indicates the migratory potential of CD163+ cells in response to 10 ng/ml TNF-α, which is impaired in the presence of the TNF-α neutralizing antibody (AT, 1 μg/ml). Arrows indicate the migrating CD163+ cells. Five fields were selected at random (at 200× magnification), and the number of CD163+ cells and total cells in each image were counted. **P<0.01..
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 used for the quantitation of CD163 (MCA342R) expressing cells in bronchiolar alvage by flow cytometry and immunofluorescence.
Image caption:
Quantification of CD163+ BAL cells by flow-cytometry. Fig (3a) shows representative flow-cytometry histograms from studied groups. Fluorescence was compared to unstained controls for 35,000 events in each histogram display, and the stained APC-CD163 positive events were compensated for unstained events by subtraction. A 5% error margin was accepted in the unstained image. GLN and IL-1/LPS showed no interaction on the percentage of CD163+ events (3b). The percentage of CD163+ events was significantly increased by GLN but not affected by IL-1/LPS. GLN and IL-1/LPS significantly interacted on the number of CD163+ macrophages (3c). CD163+ macrophage numbers were significantly increased in GLN+IL-1/LPS+ rats by both GLN and IL-1/LPS. Fig 3d shows immunofluorescence representation of CD163 stained lung regions from the GLN-IL-1/LPS+ and GLN+IL-1/LPS+ groups (white arrow points to zoomed cell in bottom right corner) (DAPI nuclear staining = blue; CD163 = red). (The significance of differences among the four groups was analyzed by two-way ANOVA. See statistical methods section for details on paired comparisons. Statistical significance was accepted as p<0.05: * compared to respective GLN- group, # compared to respective IL-1/LPS- group, ^ significant interaction between GLN and IL-1/LPS group).
From: Fernandez-Bustamante A, Agazio A, Wilson P, Elkins N, Domaleski L, He Q, et al. (2015)
Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
PLoS ONE 10(7): e0130764.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used for the identification of activated macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Representative sections showing mainly non-malignant parts of ventral prostate lobe of Dunning tumor-bearing and histologically normal prostate tissue in control rats stained to visualize CD163+ macrophages (brown, 200X magnification, the tumor border (marked T) is seen in the periphery of the sections). (B) Volume density of CD163+ macrophages in the tumor-adjacent normal prostate tissue (TINT) and in controls. a; significantly different than controls, b; significantly different than corresponding tumor at day 7, and c; significantly different than corresponding tumor at day 10, P<0.05, n = 5–13.
From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A (2015)
Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS ONE 10(11): e0141601.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used for the demonstration of CD163 expressing cells in rat kidneys by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical detection of Tgm2 and CD163. Immunohistochemical detection of Tgm2 was performed on paraffin sections of control kidneys (a) and isogenic (b) and allogenic (c) renal transplants on day 9 after transplantation. CD163-positive cells were stained on sections of isogenic (e) and allogenic (f) kidney grafts. Tgm2 or CD163 are labeled in brown; sections are lightly stained with hemalum. In (d), double-staining experiments using ED1 antibodies directed to a CD68-like antigen (in blue) and Tgm2 (in brown) reveal that a majority of Tgm2-positive intravascular leukocytes are monocytes. Representative sections out of four individual experiments are shown. The arrow points to a double-positive blood leukocyte.
From: Zakrzewicz A, Atanasova S, Padberg W, Grau V.
Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury.
Mediators Inflamm. 2015;2015:429653.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342GA) used for the demonstration of M2 macrophages in rat brain by immunohistofluorescence on cryostat sections.
Image caption:
BML‐111 increases the number of double‐positive Iba1+/ CD163+ cells 1 week post‐stroke.
(a) Representative IHC pictures taken at 40× from a vehicle‐treated rat and a BML‐111‐treated rat. DAPI marks nuclei, Iba1 (Cy3, 1:1000) marks microglia and macrophages, and CD163 (Alexa 488, 1:100) is an M2 marker. (b) Representation of infarct core (red) and peri‐infarct region (blue) in slices at bregma (±0.5 mm). Images were taken from 10 fields of view in the peri‐infarct region of vehicle and BML‐111 rats. (c) Count of Iba+/CD163+ double‐positive cells (as % of total Iba1 + cells). ***p <.01 compared to vehicle. Student's t test.
From: Hawkins KE, DeMars KM, Alexander JC, de Leon LG, Pacheco SC, Graves C, Yang C, McCrea AO, Frankowski JC, Garrett TJ, Febo M, Candelario-Jalil E.
Targeting resolution of neuroinflammation after ischemic stroke with a lipoxin A(4) analog: Protective mechanisms and long-term effects on neurological recovery.
Brain Behav. 2017 Apr 12;7(5):e00688.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in rat brain lysates by western blotting.
Image caption:
Deficiency of endogenous TSG-6 leads to an exaggerated proinflammatory microglial phenotype. Immunofluorescence labeling and quantification analysis (a–d) and western blot (e, f) and showing that inhibition of TSG-6 further enhanced the increased levels of CD86 and the enhanced CD163 levels was decreased 24 h after SAH compared with the scramble siRNA group. The samples for western blot are tissue lysates obtained from the left temporal lobe of the brain. White arrows indicate typical cells. Values of the relative densitometric analysis are expressed as mean ± SD, Scale bar = 20μm, n = 6 in each group. **P < 0.01; ***P <0.001
From: Li R, Liu W, Yin J, Chen Y, Guo S, Fan H, Li X, Zhang X, He X, Duan C.
TSG-6 attenuates inflammation-induced brain injury via modulation of microglial polarization in SAH rats through the SOCS3/STAT3 pathway.
J Neuroinflammation. 2018 Aug 20;15(1):231.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraffin embedded tissue sections and western blotting on cartilage lysate.
Image caption:
Enhanced phagocytic activity and increased CD163 and TNF-α expression in degraded TMJ cartilage. A: The gross surface morphology of rat temporomandibular joint (TMJ) condyles from control (4C) and experimental (4E, 8E, 12E) groups. Pit lesions are indicated by arrows. B: Comparison of the mRNA levels of MMP-3, MMP-9, CD163, TNF-α, IL-1, ACP-1, integrin-β1 and integrin-α4 in the condylar cartilage of control (C) and experimental (E) groups. C: Transmission electron micrographs of TMJ cartilage from control group (left top panel) and the regions with grossly damaged cartilage from experimental groups (the others panels). The apoptotic (outlined with the red dashed line) and necrotic chondrocytes are shown by arrow heads. Note that within the degraded TMJ cartilage some cells were phagocytizing neighboring apoptotic and necrotic cells. D: Serial sections of condylar cartilage from the 8-week old control (upper panels) and experimental (lower panels) groups, stained with H&E (HE), or co-stained with CD163 and TUNEL. F: fibrous layer; P: proliferative layer; H: hypertrophic layer. E: Comparison of the protein levels of CD163 and TNF-α in the condylar cartilage of control (C) and experimental (E) groups by Western blotting (left panel). Graph representing the quantification of the Western blotting results, normalized to the expression of β-actin. *P<0.05, **P<0.01. 4C: 4-week old control group; 4E: 4-week old experimental group; 8C: 8-week old control group; 8E: 8-week old experimental group; 12C: 12-week old control group; 12E: 12-week old experimental group.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used to identify macrophages in rat choroid plexus following subarachnoid hemorrhage by immunofluorescence.
Image caption:
Representative pictures illustrating double immunostaining to detect heme oxygenase-1 (HO-1) positive ED1+ (A–C) or ED2+ (D–F) macrophages in the choroid plexus 3 days after subarachnoid hemorrhage. Merged pictures with Hoechst stained nuclei (C,F) proved that heme oxygenase-1 positive Kolmer cells are either activated (ED1+) or resident (ED2+) macrophages (arrows). Scale bars – main images 80 μm; insets 20 μm.
From: Solár P, Brázda V, Levin S, Zamani A, Jančálek R, Dubový P and Joukal M (2020)
Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus.
Front. Cell. Neurosci. 14:593305.
doi: 10.3389/fncel.2020.593305
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to demonstrate M2 macrophages in rat liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis for CD68 for M1-macrophages and CD163 for M2-macrophages in rat livers after TAA injection. (A) CD68-positive macrophages are seen in the centrilobular area at 18 hr as aggregations. (B) The number of CD68-positive macrophage significantly increases at 12 hr onwards, with a peak at 18 hr. (C) There are many CD163-positive macrophages with swollen cytoplasm in the centrilobular area at12 hr. (D) The number of CD163-positive macrophages significantly increases at 12 hronwards, with a peak at 18 hr. A, C, counterstained with hematoxylin. CD, cluster of differentiation; CV, central vein; bar = 50 μm. *, P<0.05; Dunnett’s test, vs. control.
From: Kuramochi M, Izawa T, Kuwamura M, Yamate J.
Involvement of neutrophils in rat livers by low-dose thioacetamide administration.
J Vet Med Sci. 2021 Jan 20 [Epub ahead of print].
doi: 10.1292/jvms.20-0581.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) immunohistochemical staining of formalin fixed paraffin embedded human brain sections.
Image caption:
Albumin and CD163 immunoreactivity in tuberous sclerosis complex (TSC) tubers. Albumin extravasation was not observed in perituberal brain tissue of TSC patients (A). In tubers, the parenchyma was strongly positive for albumin. Albumin staining was also seen in glial cells, dysmorphic neurons and giant cells within the tuber (B). CD163‐positive perivascular macrophages were sparsely present around vessels in perituberal brain tissue (C). In tubers, CD163‐positive cells were more frequently observed around blood vessels (D) and had an activated morphology with large cell bodies and coarse processes (inset). Arrows depict dysmorphic neurons, arrowheads depict glial cells and asterisks depict giant cells. Scale bar A–D: 200 μm, scale bar inset: 25 μm.
From: Broekaart DWM, van Scheppingen J, Anink JJ, Wierts L, van Het Hof B, Jansen FE, Spliet WG, van Rijen PC, Kamphuis WW, de Vries HE, Aronica E, van Vliet EA.
Increased matrix metalloproteinases expression in tuberous sclerosis complex: modulation by microRNA 146a and 147b in vitro.
Neuropathol Appl Neurobiol. 2020 Feb;46 (2):142-59.
doi: 10.1111/nan.12572.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to label rat microglia by flow cytometry and immunofluorescence.
Image caption:
Phenotypic identity of MG d14 as M2 microglia: (A) flow cytometry analysis of MG d0 and MG d14 with CD206, CD68, and CD163 antibodies. CD206+CD68+ cells and CD206+CD163+ cells increased in MG d14; (B) fluorescence images of CD68, CCR7, CD206, and CD163 in MG d14. Left white box: magnified image of each marker positive cell.
From: Kim S, Son Y.
Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia.
Int J Mol Sci. 2021 Aug 16;22(16):8800.
doi: 10.3390/ijms22168800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Anti-inflammatory effect of EC2319. (A) Immunohistochemistry stained for CD163+ monocyte/macrophages, infiltrates of kidney sections of rats with anti-GBM GN.
From: Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, Leamon CP.
A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 2021 Aug 17;10(8):2113.
doi: 10.3390/cells10082113.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163:Alexa Fluor® 647
Mouse anti Rat CD163:Biotin
Mouse anti Rat CD163:FITC
Mouse anti Rat CD163:Low Endotoxin
Mouse anti Rat CD163
Mouse anti Rat CD163:RPE
- Product Type
- Monoclonal Antibody
- Clone
- ED2
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA342A647 | F | 100 Tests/1ml |
|
|
| MCA342B | F | 0.1 mg |
|
|
| MCA342F | F IF | 0.1 mg |
|
|
| MCA342EL | C F IF IP P* WB | 0.5 mg |
|
|
| MCA342GA | C F IF IP P* WB | 0.1 mg |
|
|
| MCA342R | C F IF IP P* WB | 0.25 mg |
|
|
| MCA342PE | F | 100 Tests |
|
Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).
Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).
Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernanant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Rat Spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD163 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Immunofluorescence | 1/10 | 1/100 | |
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/50 | 1/100 | |
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/10 |
- 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
- 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Liver
- Histology Positive Control Tissue
- Liver
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:Biotin | MCA1209B | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | F | 0.5 mg |
|
|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
|
Application Based External Images
Flow Cytometry
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
References for CD163 antibody
-
Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99. -
Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation.
Transplant Proc. 3: 3166-70. -
Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
Immunobiol. 182: 88-99. -
Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
Prog Histochem Cytochem. 27 (2): 1-65. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.
PhD Thesis. Vrije University, Amsterdam. -
Deng, X. et al. (2005) Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats.
Am J Pathol. 166 (1): 93-106. -
Piquet-Pellorce, I. et al. (2005) Identification of the leukemia inhibitory factor cell targets within the rat testis.
Biol Reprod. 72: 602-11. -
Fujita, E. et al. (2010) Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages.
Am J Pathol. 177 (3): 1143-54. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Baker, S.C. et al. (2011) Cellular integration and vascularisation promoted by a resorbable, particulate-leached, cross-linked poly(ε-caprolactone) scaffold.
Macromol Biosci. 11 (5): 618-27. -
Bedi, A. et al. (2010) Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction.
J Bone Joint Surg Am. 92: 2387-401. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Moghaddami, M. et al. (2005) MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium.
Int Immunol. 17: 1117-30. -
Wojcik, M. et al. (2012) Immunodetection of cyclooxygenase-2 (COX-2) is restricted to tissue macrophages in normal rat liver and to recruited mononuclear phagocytes in liver injury and cholangiocarcinoma.
Histochem Cell Biol. 137: 217-33. -
Kawakami, A.P. et al. (2011) Inflammatory Process Modulation by Homeopathic Arnica montana 6CH: The Role of Individual Variation.
Evid Based Complement Alternat Med. 2011: 917541. -
Kajita, M. et al. (2011) iNOS expression in vascular resident macrophages contributes to circulatory dysfunction of splanchnic vascular smooth muscle contractions in portal hypertensive rats.
Am J Physiol Heart Circ Physiol. 300: H1021-31. -
Jiao, K. et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS One. 8(1):e53312. -
Fujii, Y. et al. (2013) Effect of enzymatically modified isoquercitrin on preneoplastic liver cell lesions induced by thioacetamide promotion in a two-stage hepatocarcinogenesis model using rats.
Toxicology. 305: 30-40. -
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406. -
Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood. 109 (12): 5223-9. -
Park, E.S. et al. (2014) Establishment of a rat model for canine necrotizing meningoencephalitis (NME).
Vet Pathol. 51 (6): 1151-64. -
Keitel, V. et al. (2008) Expression and function of the bile acid receptor TGR5 in Kupffer cells.
Biochem Biophys Res Commun. 372: 78-84. -
Hozumi, Y. et al. (2015) Expression and localization of the diacylglycerol kinase family and of phosphoinositide signaling molecules in adrenal gland.
Cell Tissue Res. 362 (2): 295-305. -
Fernandez-Bustamante, A. et al. (2015) Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
PLoS One. 10 (7): e0130764. -
Adamo, H.H. et al. (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS One. 10 (11): e0141601. -
Santos, L. et al. (2016) In vitro and in vivo assessment of magnetically actuated biomaterials and prospects in tendon healing.
Nanomedicine (Lond). 11 (9): 1107-22. -
Ibarra V et al. (2016) Evaluation of the Tissue Response to Alginate Encapsulated Islets in an Omentum Pouch Model.
J Biomed Mater Res A. May 3. [Epub ahead of print] -
Tentillier N et al. (2016) Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model.
J Neurosci. 36 (36): 9375-90. -
Zakrzewicz, A. et al. (2015) Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury.
Mediators Inflamm. 2015: 429653. -
Rave-Fränk M et al. (2013) Rat model of fractionated (2 Gy/day) 60 Gy irradiation of the liver: long-term effects.
Radiat Environ Biophys. 52 (3): 321-38. -
Wang, M. et al. (2017) Characterization of the Micro-Environment of the Testis that Shapes the Phenotype and Function of Testicular Macrophages.
J Immunol. May 1. pii: 1700162. [Epub ahead of print] -
Stavenuiter, A.W. et al. (2015) Protective Effects of Paricalcitol on Peritoneal Remodeling during Peritoneal Dialysis.
Biomed Res Int. 2015: 468574. -
Hawkins, K.E. et al. (2017) Targeting resolution of neuroinflammation after ischemic stroke with a lipoxin A4 analog: Protective mechanisms and long-term effects on neurological recovery.
Brain Behav. 7 (5): e00688. -
Almahrog, A.J. et al. (2016) In vivo. association of immunophenotyped macrophages expressing CD163 with PDGF-β in gingival overgrowth-induced by three different categories of medications.
J Oral Biol Craniofac Res. 6 (1): 10-7. -
Han, T.T. et al. (2015) Adipose-derived stromal cells mediate in vivo adipogenesis, angiogenesis and inflammation in decellularized adipose tissue bioscaffolds.
Biomaterials. 72: 125-37. -
Pannell, M. et al. (2016) Adoptive transfer of M2 macrophages reduces neuropathic pain via opioid peptides.
J Neuroinflammation. 13 (1): 262. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Bloomer, S.A. et al. (2019) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride
Histochem Cell Biol. 25 Oct [Epub ahead of print]. -
Azam, M. et al. (2019) Addition of 2-deoxy-d-ribose to clinically used alginate dressings stimulates angiogenesis and accelerates wound healing in diabetic rats.
J Biomater Appl. 34 (4): 463-475. -
Bhandari, S. et al. (2020) Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages.
BMC Mol Cell Biol. 21 (1): 85. -
Solár, P. et al. (2020) Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus.
Front Cell Neurosci. 14: 593305. -
Nishida, Y. et al. (2020) Intra-Articular Injection of Stromal Cell-Derived Factor 1α Promotes Meniscal Healing via Macrophage and Mesenchymal Stem Cell Accumulation in a Rat Meniscal Defect Model.
Int J Mol Sci. 21(15):5454. -
Bloomer, S.A. et al. (2020) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride.
Histochem Cell Biol. 153 (1): 37-48. -
Kuramochi, M. et al. (2021) Involvement of neutrophils in rat livers by low-dose thioacetamide administration.
J Vet Med Sci. Jan 20 [Epub ahead of print]. -
Broekaart, D.W.M. et al. (2020) Increased matrix metalloproteinases expression in tuberous sclerosis complex: modulation by microRNA 146a and 147b in vitro..
Neuropathol Appl Neurobiol. 46 (2): 142-159. -
Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
Muscle Nerve. 48 (3): 403-14. -
Li, R. et al. (2020) Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization.
Arthritis Res Ther. 22 (1): 75. -
Matsumoto, K. et al. (2021) Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis.
J Pharmacol Sci. 146 (3): 125-35. -
Koppe, C. et al. (2021) Local Inflammatory Response after Intramuscularly Implantation of Anti-Adhesive Plasma-Fluorocarbon-Polymer Coated Ti6AI4V Discs in Rats.
Polymers (Basel). 13(16):2684. -
Kim, S. & Son, Y. (2021) Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro. through Crosstalk between Astrocytes and Microglia.
Int J Mol Sci. 22 (16): 8800. -
Takei, R. et al. (2021) Dynamic switch of immunity and antitumor effects of metformin in rat spontaneous esophageal carcinogenesis.
Cancer Immunol Immunother. Aug 16 [Epub ahead of print]. -
Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
J Vet Med Sci. 83 (9): 1435-42. -
Garcia, G.E. et al. (2021) A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 10(8): 2113.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Our CD163 (ED2) Antibody has been referenced in >116 publications*
*Based on June 2020 data from CiteAb's antibody search engine.
