CD163 antibody | ED2
Mouse anti Rat CD163, clone ED2 (MCA342R) used for the demonstration of infiltrating macrophages in corneal graft tissue by immunohistochemistry on acetone fixed cryosections.
Image caption:
Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01). F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by immunofluorescence microscopy and flow cytometry.
Image caption:
Increase in CD163+ cells with enhanced phagocytic activity in experimentally-induced arthritic cartilage of rat TMJs. A: Flow cytometry analysis and comparison of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within isolated type II collagen expressing (Col-II+) cells from TMJ cartilage from the 8-week experimental group and their age-matched controls. B: Confocal microscope images of the CD163+ cells and assessment of their phagocytic activity in primary cells isolated from TMJ cartilage. The images reveal an increase in CD163+ cells and enhanced co-localization with the FITC-labeled cell debris in 8-week experimental group compared with the age-matched controls. C: Serial confocal images (1–4) of the primary cells isolated from TMJ cartilage of 3-week old rats co-cultured with DiO-labeled cellular debris. Sections were stained with a CD163 antibody and a Cy3-conjugated secondary antibody. Note that the CD163+ cells showed membrane staining (red) and the cell debris (green) was located inside the cell membrane. D: Dynamic observation of the phagocytic process involving living CD163+ cells sorted from TMJ cartilage engulfing cellular debris. Note that the DiO-labeled debris is undergoing phagocytosis by the CD163+ cell indicated within the white box. E: Comparison of the nitric oxide (NO) concentration and amount of intracellular reactive oxygen species (ROS) in the primary cells isolated from TMJ cartilage from 8-week experimental group and their age-matched controls. *P<0.05.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraqffin embedded tissue sections and and flow cytometry on isolated cells.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by flow cytometry.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by flow cytometry on isolated cells.
Image caption:
Exogenous TNF-α increased CD163 expression in primary chondrocytes from TMJ cartilage of 3-week old rats. A: The primary cells isolated from TMJ cartilage of 3-week old rats were positive for type II collagen (Col-II) and aggrecan, as detected by immunofluorescence and toluidine blue, respectively (400× magnification). B: A time-course of induction of CD163 mRNA expression in primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α. C–D: Flow cytometric analysis and graphical representation of the percentage of CD163+ cells within the primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunofluorescence microscopy on isolated cells.
Image caption:
TNF-α increased the phagocytic and migratory activities of CD163+ cells isolated from rat TMJ cartilage. A–B: Confocal microscope images (A) and graphical representation (B) of the numbers of CD163+ cells and their phagocytic activity within primary cells isolated from TMJ cartilage and treated with vehicle, TNF-α alone, or TNF-α and a CD163 neutralizing antibody. The co-localization of the CD163+ cell with DiO-labeled cell debris (arrows), indicates that the cell debris is undergoing phagocytosis by the CD163+ cells, as shown in the insets. Bar: 50 μm. C: Transwell assay combined with immunohistochemical staining of CD163 indicates the migratory potential of CD163+ cells in response to 10 ng/ml TNF-α, which is impaired in the presence of the TNF-α neutralizing antibody (AT, 1 μg/ml). Arrows indicate the migrating CD163+ cells. Five fields were selected at random (at 200× magnification), and the number of CD163+ cells and total cells in each image were counted. **P<0.01..
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 used for the quantitation of CD163 (MCA342R) expressing cells in bronchiolar alvage by flow cytometry and immunofluorescence.
Image caption:
Quantification of CD163+ BAL cells by flow-cytometry. Fig (3a) shows representative flow-cytometry histograms from studied groups. Fluorescence was compared to unstained controls for 35,000 events in each histogram display, and the stained APC-CD163 positive events were compensated for unstained events by subtraction. A 5% error margin was accepted in the unstained image. GLN and IL-1/LPS showed no interaction on the percentage of CD163+ events (3b). The percentage of CD163+ events was significantly increased by GLN but not affected by IL-1/LPS. GLN and IL-1/LPS significantly interacted on the number of CD163+ macrophages (3c). CD163+ macrophage numbers were significantly increased in GLN+IL-1/LPS+ rats by both GLN and IL-1/LPS. Fig 3d shows immunofluorescence representation of CD163 stained lung regions from the GLN-IL-1/LPS+ and GLN+IL-1/LPS+ groups (white arrow points to zoomed cell in bottom right corner) (DAPI nuclear staining = blue; CD163 = red). (The significance of differences among the four groups was analyzed by two-way ANOVA. See statistical methods section for details on paired comparisons. Statistical significance was accepted as p<0.05: * compared to respective GLN- group, # compared to respective IL-1/LPS- group, ^ significant interaction between GLN and IL-1/LPS group).
From: Fernandez-Bustamante A, Agazio A, Wilson P, Elkins N, Domaleski L, He Q, et al. (2015)
Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
PLoS ONE 10(7): e0130764.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used for the identification of activated macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Representative sections showing mainly non-malignant parts of ventral prostate lobe of Dunning tumor-bearing and histologically normal prostate tissue in control rats stained to visualize CD163+ macrophages (brown, 200X magnification, the tumor border (marked T) is seen in the periphery of the sections). (B) Volume density of CD163+ macrophages in the tumor-adjacent normal prostate tissue (TINT) and in controls. a; significantly different than controls, b; significantly different than corresponding tumor at day 7, and c; significantly different than corresponding tumor at day 10, P<0.05, n = 5–13.
From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A (2015)
Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS ONE 10(11): e0141601.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used for the demonstration of CD163 expressing cells in rat kidneys by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical detection of Tgm2 and CD163. Immunohistochemical detection of Tgm2 was performed on paraffin sections of control kidneys (a) and isogenic (b) and allogenic (c) renal transplants on day 9 after transplantation. CD163-positive cells were stained on sections of isogenic (e) and allogenic (f) kidney grafts. Tgm2 or CD163 are labeled in brown; sections are lightly stained with hemalum. In (d), double-staining experiments using ED1 antibodies directed to a CD68-like antigen (in blue) and Tgm2 (in brown) reveal that a majority of Tgm2-positive intravascular leukocytes are monocytes. Representative sections out of four individual experiments are shown. The arrow points to a double-positive blood leukocyte.
From: Zakrzewicz A, Atanasova S, Padberg W, Grau V.
Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury.
Mediators Inflamm. 2015;2015:429653.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342GA) used for the demonstration of M2 macrophages in rat brain by immunohistofluorescence on cryostat sections.
Image caption:
BML‐111 increases the number of double‐positive Iba1+/ CD163+ cells 1 week post‐stroke.
(a) Representative IHC pictures taken at 40× from a vehicle‐treated rat and a BML‐111‐treated rat. DAPI marks nuclei, Iba1 (Cy3, 1:1000) marks microglia and macrophages, and CD163 (Alexa 488, 1:100) is an M2 marker. (b) Representation of infarct core (red) and peri‐infarct region (blue) in slices at bregma (±0.5 mm). Images were taken from 10 fields of view in the peri‐infarct region of vehicle and BML‐111 rats. (c) Count of Iba+/CD163+ double‐positive cells (as % of total Iba1 + cells). ***p <.01 compared to vehicle. Student's t test.
From: Hawkins KE, DeMars KM, Alexander JC, de Leon LG, Pacheco SC, Graves C, Yang C, McCrea AO, Frankowski JC, Garrett TJ, Febo M, Candelario-Jalil E.
Targeting resolution of neuroinflammation after ischemic stroke with a lipoxin A(4) analog: Protective mechanisms and long-term effects on neurological recovery.
Brain Behav. 2017 Apr 12;7(5):e00688.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342GA) used for the evaluation of CD163 expression in rat brain lysates by western blotting.
Image caption:
Deficiency of endogenous TSG-6 leads to an exaggerated proinflammatory microglial phenotype. Immunofluorescence labeling and quantification analysis (a–d) and western blot (e, f) and showing that inhibition of TSG-6 further enhanced the increased levels of CD86 and the enhanced CD163 levels was decreased 24 h after SAH compared with the scramble siRNA group. The samples for western blot are tissue lysates obtained from the left temporal lobe of the brain. White arrows indicate typical cells. Values of the relative densitometric analysis are expressed as mean ± SD, Scale bar = 20μm, n = 6 in each group. **P < 0.01; ***P <0.001
From: Li R, Liu W, Yin J, Chen Y, Guo S, Fan H, Li X, Zhang X, He X, Duan C.
TSG-6 attenuates inflammation-induced brain injury via modulation of microglial polarization in SAH rats through the SOCS3/STAT3 pathway.
J Neuroinflammation. 2018 Aug 20;15(1):231.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraffin embedded tissue sections and western blotting on cartilage lysate.
Image caption:
Enhanced phagocytic activity and increased CD163 and TNF-α expression in degraded TMJ cartilage. A: The gross surface morphology of rat temporomandibular joint (TMJ) condyles from control (4C) and experimental (4E, 8E, 12E) groups. Pit lesions are indicated by arrows. B: Comparison of the mRNA levels of MMP-3, MMP-9, CD163, TNF-α, IL-1, ACP-1, integrin-β1 and integrin-α4 in the condylar cartilage of control (C) and experimental (E) groups. C: Transmission electron micrographs of TMJ cartilage from control group (left top panel) and the regions with grossly damaged cartilage from experimental groups (the others panels). The apoptotic (outlined with the red dashed line) and necrotic chondrocytes are shown by arrow heads. Note that within the degraded TMJ cartilage some cells were phagocytizing neighboring apoptotic and necrotic cells. D: Serial sections of condylar cartilage from the 8-week old control (upper panels) and experimental (lower panels) groups, stained with H&E (HE), or co-stained with CD163 and TUNEL. F: fibrous layer; P: proliferative layer; H: hypertrophic layer. E: Comparison of the protein levels of CD163 and TNF-α in the condylar cartilage of control (C) and experimental (E) groups by Western blotting (left panel). Graph representing the quantification of the Western blotting results, normalized to the expression of β-actin. *P<0.05, **P<0.01. 4C: 4-week old control group; 4E: 4-week old experimental group; 8C: 8-week old control group; 8E: 8-week old experimental group; 12C: 12-week old control group; 12E: 12-week old experimental group.
From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013)
The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti rat CD163, clone ED2 (MCA342R) used to identify macrophages in rat choroid plexus following subarachnoid hemorrhage by immunofluorescence.
Image caption:
Representative pictures illustrating double immunostaining to detect heme oxygenase-1 (HO-1) positive ED1+ (A–C) or ED2+ (D–F) macrophages in the choroid plexus 3 days after subarachnoid hemorrhage. Merged pictures with Hoechst stained nuclei (C,F) proved that heme oxygenase-1 positive Kolmer cells are either activated (ED1+) or resident (ED2+) macrophages (arrows). Scale bars – main images 80 μm; insets 20 μm.
From: Solár P, Brázda V, Levin S, Zamani A, Jančálek R, Dubový P and Joukal M (2020)
Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus.
Front. Cell. Neurosci. 14:593305.
doi: 10.3389/fncel.2020.593305
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to demonstrate M2 macrophages in rat liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis for CD68 for M1-macrophages and CD163 for M2-macrophages in rat livers after TAA injection. (A) CD68-positive macrophages are seen in the centrilobular area at 18 hr as aggregations. (B) The number of CD68-positive macrophage significantly increases at 12 hr onwards, with a peak at 18 hr. (C) There are many CD163-positive macrophages with swollen cytoplasm in the centrilobular area at12 hr. (D) The number of CD163-positive macrophages significantly increases at 12 hronwards, with a peak at 18 hr. A, C, counterstained with hematoxylin. CD, cluster of differentiation; CV, central vein; bar = 50 μm. *, P<0.05; Dunnett’s test, vs. control.
From: Kuramochi M, Izawa T, Kuwamura M, Yamate J.
Involvement of neutrophils in rat livers by low-dose thioacetamide administration.
J Vet Med Sci. 2021 Jan 20 [Epub ahead of print].
doi: 10.1292/jvms.20-0581.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) immunohistochemical staining of formalin fixed paraffin embedded human brain sections.
Image caption:
Albumin and CD163 immunoreactivity in tuberous sclerosis complex (TSC) tubers. Albumin extravasation was not observed in perituberal brain tissue of TSC patients (A). In tubers, the parenchyma was strongly positive for albumin. Albumin staining was also seen in glial cells, dysmorphic neurons and giant cells within the tuber (B). CD163‐positive perivascular macrophages were sparsely present around vessels in perituberal brain tissue (C). In tubers, CD163‐positive cells were more frequently observed around blood vessels (D) and had an activated morphology with large cell bodies and coarse processes (inset). Arrows depict dysmorphic neurons, arrowheads depict glial cells and asterisks depict giant cells. Scale bar A–D: 200 μm, scale bar inset: 25 μm.
From: Broekaart DWM, van Scheppingen J, Anink JJ, Wierts L, van Het Hof B, Jansen FE, Spliet WG, van Rijen PC, Kamphuis WW, de Vries HE, Aronica E, van Vliet EA.
Increased matrix metalloproteinases expression in tuberous sclerosis complex: modulation by microRNA 146a and 147b in vitro.
Neuropathol Appl Neurobiol. 2020 Feb;46 (2):142-59.
doi: 10.1111/nan.12572.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to label rat microglia by flow cytometry and immunofluorescence.
Image caption:
Phenotypic identity of MG d14 as M2 microglia: (A) flow cytometry analysis of MG d0 and MG d14 with CD206, CD68, and CD163 antibodies. CD206+CD68+ cells and CD206+CD163+ cells increased in MG d14; (B) fluorescence images of CD68, CCR7, CD206, and CD163 in MG d14. Left white box: magnified image of each marker positive cell.
From: Kim S, Son Y.
Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia.
Int J Mol Sci. 2021 Aug 16;22(16):8800.
doi: 10.3390/ijms22168800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Anti-inflammatory effect of EC2319. (A) Immunohistochemistry stained for CD163+ monocyte/macrophages, infiltrates of kidney sections of rats with anti-GBM GN.
From: Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, Leamon CP.
A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 2021 Aug 17;10(8):2113.
doi: 10.3390/cells10082113.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163 antibody, clone ED2 (MCA342R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Infiltrating macrophages after kidney transplantation in vehicle and Rapa treated rats.
(A) ED1 and (B) ED2 positive macrophages in the cortex calculated as percentage of positive interstitial cells by immunohistochemistry. Representative photomicrographs are shown. n = 3. (C) Comparison of ED1 and ED2 positive infiltrating cells on day 7 in vehicle and Rapa treated rats. Cortex and outer medulla were analyzed together. n = 6–8. (D) Semi-quantitative real-time PCR for mRNA transcripts of inducible NO synthase (iNOS) in whole tissue extracts from kidney allografts. n = 4–6. *P<0.05, **P<0.01, ***P<0.001.
From: Hoff U, Markmann D, Thurn-Valassina D, Nieminen-Kelhä M, Erlangga Z, Schmitz J, et al.. (2022)
The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
PLoS ONE 17(4): e0266319.
doi: 10.1371/journal.pone.0266319
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
RPE conjugated Mouse anti Rat CD163 antibody, clone ED2 (MCA342PE) used to evaluate CD163 expression on isolated spleen mononuclear cells by flow cytometry.
Image caption:
Surfactant modification improved the disease course of EAN. Spleen mononuclear cells (MNCs) were isolated from the P2-Peptide and treatment groups at day 17 of post-immunizations. With flow cytometry, these monocytes were identified by gating against the side and forward scatter. The percentage (Q2) of CD68 and CD163 of each NPs type was determined and compared with the P2-peptide group. A distinct reduced percentage of activated macrophages (CD11b+CD68+) was observed with the treatment of NP-PVA130. A slightly higher CD11b+CD163 cell percentage was observed with the treatment of NP-PVA130. No significant differences were observed between the respective groups.
From: Elahi E, Ali ME, Zimmermann J, Getts DR, Müller M, Lamprecht A.
Immune Modifying Effect of Drug Free Biodegradable Nanoparticles on Disease Course of Experimental Autoimmune Neuritis.
Pharmaceutics. 2022 Nov 8;14(11):2410.
doi: 10.3390/pharmaceutics14112410.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alexa Fluor 647 conjugated Mouse anti Rat CD163 antibody, clone ED2 (MCA342A647) used to assess CD163 expression by flow cytometry.
Image caption:
BTK inhibition regulated macrophage polarization in MCT-induced PAH.
Expression of CD68+ (macrophages), CD68+CD86+ (M1), and CD68+CD163+ (M2) in bronchoalveolar lavage was evaluated by flow cytometry analysis. (b–d) Representative images of flow cytometry analysis.
From: Yu M, Wu X, Peng L, Yang M, Zhou H, Xu J, Wang J, Wang H, Xie W, Kong H.
Inhibition of Bruton's Tyrosine Kinase Alleviates Monocrotaline-Induced Pulmonary Arterial Hypertension by Modulating Macrophage Polarization.
Oxid Med Cell Longev. 2022 Aug 29;2022:6526036.
doi: 10.1155/2022/6526036.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD163
- Product Type
- Monoclonal Antibody
- Clone
- ED2
- Isotype
- IgG1
- Specificity
- CD163
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al. |
Our CD163 (ED2) Antibody has been referenced in >116 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat spleen cell homogenate.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
1/50 | 1/100 |
| Immunohistology - Paraffin 1 | |
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| Immunoprecipitation | |
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| Western Blotting | |
- 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Histology Positive Control Tissue
- Liver
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
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Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99.
References for CD163 antibody
-
Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
Immunobiol. 182: 88-99. -
Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
Prog Histochem Cytochem. 27 (2): 1-65. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Muller, D.N. et al. (2002) Immunosuppressive treatment protects against angiotensin II-induced renal damage.
Am J Pathol. 161: 1679-93. -
Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.
PhD Thesis. Vrije University, Amsterdam. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Moghaddami, M. et al. (2005) MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium.
Int Immunol. 17: 1117-30. -
Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.
J Exp Med. 202: 919-29.
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- Synonyms
- ED2
- RRID
- AB_321966
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