CD152 antibody | WKH203

Mouse anti Rat CD152

Product Type
Monoclonal Antibody
Clone
WKH203
Isotype
IgG1
Specificity
CD152

Product Code Applications Pack Size List Price Your Price Qty
MCA2092
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * WB 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD152 antibody, clone WKH203 recognizes rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and by regulatory T-lymphocytes (Lin and Hunig 2003).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified rCTLA-4hIg fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c were fused with cells of the X63-Ag8-653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/20
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Lin, C.H. & Hünig, T. (2003) Efficient expansion of regulatory T cells in vitro and in vivo with a CD28 superagonist.
    Eur J Immunol. 33 (3): 626-38.

References for CD152 antibody

  1. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
    Blood. 102 (5): 1764-70.
  2. Blewett, H.J. et al. (2009) Vaccenic acid favourably alters immune function in obese JCR:LA-cp rats.
    Br J Nutr. 102 (4): 526-36.
  3. Almolda, B. et al. (2010) Activated microglial cells acquire an immature dendritic cell phenotype and may terminate the immune response in an acute model of EAE.
    J Neuroimmunol. 223 (1-2): 39-54.
  4. Beyersdorf, N. et al. (2005) Selective targeting of regulatory T cells with CD28 superagonists allows effective therapy of experimental autoimmune encephalomyelitis.
    J Exp Med. 202 (3): 445-55.
  5. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
    Blood. 102 (5): 1764-70.
  6. Hara, Y. et al. (2006) Anergic lymphocytes generated by blocking CD28 and ICOS pathways in vitro prolong rat cardiac graft survival.
    Int Immunopharmacol. 6 (7): 1143-51.

Flow Cytometry

Synonyms
CLTA-4
CTLA-4
RRID
AB_2087798
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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