CD11b antibody | OX-42

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.25 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 1 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.1 mg loader
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Mouse anti Rat CD11b, clone OX-42 recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinson et al.1986).

Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinson et al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996).

Target Species
Product Form
Purified IgG - liquid
MCA275R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA275G, MCA275GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Resident rat peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative /positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD11b antibody

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Flow Cytometry

Functional Assays


Immunohistology - Frozen

Immunohistology - Paraffin

Integrin Alpha M Chain


155655 1606


156705 158804 164596 1708


154816 158080 1708

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