CD11b antibody | OX-42
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used for the evaluation of CD11b expression on rat microglia by immunofluorescence.
Image caption:
Double immunostaining to detect CD200 protein in astrocytes and neurons as well as CD200R in microglia. Double immunostaining of sections through DH of L4–L5 spinal cord segments from sCCI-operated and ACSF-treated rats. Immunofluorescence for CD200 (a) and GFAP (b) are colocalized (c), evidencing that CD200 protein is expressed by astrocytes (arrows). In addition, double immunostaining for CD200 (d) and NeuN (e) detected a presence of CD200 protein also in neurons (f, arrows). Immunolabeling for CD200R (g) and OX42 (h) was colocalized, illustrating that activated microglial cells display CD200R protein (i, arrow). Merged figures (c, f, i) contain also blue channel of Hoechst stained nuclei. Scale bars = 30 μm
From: Hernangómez M, Klusáková I, Joukal M, Hradilová-Svíženská I, Guaza C, Dubový P.
CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model.
J Neuroinflammation. 2016 Feb 18;13(1):43.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used for the identification of rat microglia byImmunohistochemistry on brain cryostat sections.
Image caption
Immunohistochemical analysis of microglial activation and neuronal loss on day 3 post-treatment with UCN. Representative photomicrographs show: (A) low power images of peri-hematomal regions as indicated by squares; (B) high power images stained with OX-42; (C) ED-1; and (D) NeuN. (E) Average cell numbers (OX 42+, ED-1+, and NeuN+) in each plane (total 6 mm2) was calculated from 54 squares in 9 planes. Values are shown as mean ± SD, calculated and analyzed by Student's t test. *p<0.05 vs. ICH + saline group.
From: Liew HK, Pang CY, Hsu CW, Wang MJ, Li TY, Peng HF, Kuo JS, Wang JY.
Systemic administration of urocortin after intracerebral hemorrhage reduces neurological deficits and neuroinflammation in rats.
J Neuroinflammation. 2012 Jan 19;9:13.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA)used for the demonstration of rat microglia by immunofluorescence on rat brain sections.
Image caption
Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).
From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN.
Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration.
BMC Neurosci. 2010 Feb 5;11:13.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used for the identification of rat microglia by immunofluorescence on rat rubrospinal sections.
Image caption
Rubrospinal injury produces little microglial activation and no accumulation of T-cells in the red nucleus. a, b: Representative images show there is little difference in CD11b immunoreactivity for microglia in the red nucleus (*) one week following axotomy. c, d: confocal images of β thymosin immunoreactive microglia in the red nucleus 3 weeks after injury. e: a rare CD6-positive T lymphocyte in the red nucleus of an unoperated rat. f: one week after injury no T lymphocytes can be identified in the red nucleus around the axotomized rubrospinal neurons, which can be recognised by their expression of ATF3 (green). Neuronal cytoplasm has been visualised by high gain in the red signal; this does not represent CD6 signal.
From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN.
Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration.
BMC Neurosci. 2010 Feb 5;11:13.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used for the identificaton of rat microglia by immunofluorescence on rat brain sections.
Image caption
CD11b immunoreactive (red) microglia in the motor cortex following cervical corticospinal tract injury. Representative images of motor cortex - a, b: one week (deconvolved images); c, d: two weeks following corticospinal tract injury. The corticospinal neurons were retrogradely labelled with fluorogold applied to the spinal cord at the time of injury. There is little sign of microglial activation. The inset in (c) shows a CD6-positive T-cell in the motor cortex, but no accumulation of such cells was detected.
From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN.
Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration.
BMC Neurosci. 2010 Feb 5;11:13.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used for the identification of microglia by immunofluorescence on in vitro cultured cells.
Image caption
MBP binds to the extracellular surface of neuronal plasma membrane. (B) Primary astrocytes, microglia, oligodendrocytes and the endothelial cell line bEND.3 were incubated with HBSS or 50 μg/mL MBP for 30 min, washed with HBSS for three times, 5 min each time, and then stained for MBP surface binding (without permeabilization). No surface-bound MBP was detected (green). GFAP and CD11b were used as markers of astrocytes and microglia, respectively (red). Scale bar, 50 μm.
From: Zhang J, Sun X, Zheng S, Liu X, Jin J, et al. (2014)
Myelin Basic Protein Induces Neuron-Specific Toxicity by Directly Damaging the Neuronal Plasma Membrane.
PLoS ONE 9(9): e108646.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used for the identification of microglia in rat brain by immunofluorescence on formalin fixed cryostat sections.
Image caption:
Cellular localization of 3MST using dual-fluorescent immunohistochemical labelling on rat cortical sections. 3MST does not colocalize with OX42-immunoreactive mciroglial cells (red). Scale bar = 50μm. All sections were observed with an Olympus confocal fluorescent microscope.
From: Zhao H, Chan S-J, Ng Y-K, Wong PT-H (2013)
Brain 3-Mercaptopyruvate Sulfurtransferase (3MST):
Cellular Localization and Downregulation after Acute Stroke.
PLoS ONE 8(6): e67322.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used for the evaluation of CD11b expression on rat microglia by immunofluorescence.
Image caption:
Double immunostaining to detect CD200 protein in astrocytes and neurons as well as CD200R in microglia. Double immunostaining of sections through DH of L4–L5 spinal cord segments from sCCI-operated and ACSF-treated rats. Immunofluorescence for CD200 (a) and GFAP (b) are colocalized (c), evidencing that CD200 protein is expressed by astrocytes (arrows). In addition, double immunostaining for CD200 (d) and NeuN (e) detected a presence of CD200 protein also in neurons (f, arrows). Immunolabeling for CD200R (g) and OX42 (h) was colocalized, illustrating that activated microglial cells display CD200R protein (i, arrow). Merged figures (c, f, i) contain also blue channel of Hoechst stained nuclei. Scale bars = 30 μm
From: Hernangómez M, Klusáková I, Joukal M, Hradilová-Svíženská I, Guaza C, Dubový P.
CD200R1 agonist attenuates glial activation, inflammatory reactions, and hypersensitivity immediately after its intrathecal application in a rat neuropathic pain model.
J Neuroinflammation. 2016 Feb 18;13(1):43.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used for the identification of microglia by immunofluorescence on rat brain sections.
Image caption
Representative images of microglia in the TRN following nerve implantation into the thalamus. (a and b) are deconvolved digital images, (c) is a non-deconvolved image and (d) is a confocal image. The neurons which regenerated axons into the grafts were retrogradely labelled with fluorogold (a, b and c) or CTB (d). There is clearly increased CD11b activity in the TRN around the retrogradely - labelled neurons which are regenerating axons (b) compared with the contralateral (uninjured) side (a). There was close contract between microglia and TRN neurons with regenerating axons (arrows in c and d). The scale bar in (a) also applies to (b).
From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN.
Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration.
BMC Neurosci. 2010 Feb 5;11:13
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (strong>MCA275GA) used for the detection of rat neurons by immunofluorescence on rat brain sections.
Image caption
Microglia in the red nucleus (a-d and inset) and motor cortex (e, f) 4 weeks after nerve graft implantation into the rubrospinal tract and cervical dorsal columns respectively. Neurons with regenerating axons were retrogradely labelled with fluorogold. Microglia established close contacts with regenerating rubrospinal neurons (arrows), which also expressed ATF3 (inset). No retrogradely labelled corticospinal neurons were detected (e and f) and there was no microglial activation in the motor cortex. (a-c) are non-deconvolved and (d-f plus inset) are deconvolved images. The scale bar in (a) also applies to (b).
From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN.
Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration.
BMC Neurosci. 2010 Feb 5;11:13.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used for the demonstration of infiltrating cells in rat brain by immunofluorescence on vibratome sections.
Image caption:
Expression of monocyte chemoattractant CCL2 in the injured brain after mTBI.
In these experiments, the depth of brain deformation was set at 1 mm, and the images shown were acquired at 6 h post-mTBI. (A, B) Double immunostaining of injured brains with anti-CCL2 antibody and an antibody to S100B, an astrocyte marker. Note that CCL2 is not only expressed ipsilaterally in astrocyte processes (arrowheads), but is also carried by neutrophils initially entering the subarachnoid space (SAS) near the injury site and then invading the injured brain parenchyma (arrows). (C) Double immunostaining of neutrophils entering the SAS with anti-CCL2 antibody and an antibody to CD11b, a neutrophil marker. (D, E) The astrocytic expression of CCL2 in the vicinity of the cistern of velum interpositum (CVI). Double immunostaining of injured brains was performed with anti-CCL2 antibody and an antibody to glial fibrillary acidic protein (GFAP), an astrocyte marker. Bars: panels A, B, D, E, 25 μm; panel C, 10 μm.
From: Citation: Szmydynger-Chodobska J, Shan R, Thomasian N, Chodobski A (2016)
The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury.
PLoS ONE 11(12): e0167677.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used for the demonstration of microglia in rat brain by immunofluorescence.
Image caption:
XRN2 and PAPD4 do not accumulate in microglial cells.
(A) In order to verify the presence of XRN2 (green) in microglial cells, we employed anti-OX42 (red) in double labeling experiments performed in vertical sections of rat adult retinas counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). (B–D) In high magnification of selected areas, we identified the nuclei of microglial cells, which were not stained for XRN2 (white arrow), in spite of the positive labeling in surrounding areas (white arrowhead). (E) We also examined the presence of PAPD4 in microglial cells. (F–H) In high magnification of selected areas, we were able to identify nuclei of microglial cells, which were not positive for PAPD4 (red arrow), although the presence of this protein was detected in cells located nearby (red arrowhead). Scale bar: 60 μm.
From: de Sousa É, Walter LT, Higa GSV, Casado OAN, Kihara AH (2013)
Developmental and Functional Expression of miRNA-Stability Related Genes in the Nervous System.
PLoS ONE 8(5): e56908.
https://doi.org/10.1371/journal.pone.0056908
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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Image caption:
Fluorescent photomicrographs illustrating IB4 (green) and OX42 (red) staining in the spinal dorsal or ventral horn on the control side (a, c, e, g) and ipsilateral to transection (b, d, f, h) of the sciatic nerve in the L4 segment of the spinal cord. Note the disappearance of IB4 staining of C-fiber primary afferent terminals from somatotopically relating projection areas of the sciatic nerve (b, f). The increase in microglial density is confined to this area in laminae I–II and to regions of the deeper laminae III–IV just ventrally to the “IB4 gap.” Note the intense perineuronal microglia reaction around sciatic motoneurons in the ventral horn of the spinal cord ipsilateral to sciatic nerve transection (h). The scale bar in (h) indicates 100 μm and holds for all photomicrographs.
From: Szeredi ID, Jancsó G, Oszlács O, Sántha P.
Prior perineural or neonatal treatment with capsaicin does not alter the development of spinal microgliosis induced by peripheral nerve injury.
Cell Tissue Res. 2020 Sep 22.
doi: 10.1007/s00441-020-03285-8.
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Mouse anti Rat CD11b antibody, clone OX-42 (MCA275G) used to label microglia in rat brain by immunofluorescence.
Image caption:
sEH inhibition reduces microglial recruitment and CD74 expression. Visualization of microglia. Whole mount retinal preparations (400x magnification) showing microglia (CD11b+/CD74+) in the superficial and deep layer of retinae from SD rats, and vehicle or sEH-I-treated PKD rats. Scale bar = 75 μm, CD11b = green, CD74 = red, lectin = blue.
From: Lin J, Hu J, Schlotterer A, Wang J, Kolibabka M, Awwad K, Dietrich N, Breitschopf K, Wohlfart P, Kannt A, Lorenz K, Feng Y, Popp R, Hoffmann S, Fleming I, Hammes HP.
Protective effect of Soluble Epoxide Hydrolase Inhibition in Retinal Vasculopathy associated with Polycystic Kidney Disease.
Theranostics. 2020 Jun 22;10(17):7857-71.
doi: 10.7150/thno.43154.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275G) used to label microglia in rat brain by immunofluorescence.
Image caption:
19,20-EDP and 19,20-DHDP impact Notch signaling and microglial activation. (A-B) For ex vivo retinal explants cultures, retinae from one-month old PKD rats were incubated for 24 hours in 10 μmol/L sEH-I with 0.03% DMSO (CTL), 3 μmol/L EDP, 3 μmol/L DHDP or 10 μmol/L DAPT. (A) CD74+ (red) and CD11b+ (green) retinal microglia, the yellow arrows indicate CD74+ microglia; bar = 50 μm.
From: Lin J, Hu J, Schlotterer A, Wang J, Kolibabka M, Awwad K, Dietrich N, Breitschopf K, Wohlfart P, Kannt A, Lorenz K, Feng Y, Popp R, Hoffmann S, Fleming I, Hammes HP.
Protective effect of Soluble Epoxide Hydrolase Inhibition in Retinal Vasculopathy associated with Polycystic Kidney Disease.
Theranostics. 2020 Jun 22;10(17):7857-71.
doi: 10.7150/thno.43154.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify microglia in vitro by immunofluorescence.
Image caption:
Dissociated and ex vivo cultures of peeled-off leptomeninges.
(C) After fixation, the aggregates were sectioned and immunostained with antibodies against CD11b (green), a marker of microglia/macrophages, and Ki67 (red). Many CD11b+ amoeboid cells expressed Ki67 in the aggregates. Nuclei were stained blue by Hoechst 33258. Scale bar = 50 μm.
From: Tanaka J, Takahashi H, Yano H, Nakanishi H.
Generation of CSF1-Independent Ramified Microglia-Like Cells from Leptomeninges In Vitro..
Cells. 2020 Dec 25;10(1):E24.
doi: 10.3390/cells10010024.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify microglia in vitro by immunofluorescence.
Image caption:
Dissociated and ex vivo cultures of peeled-off leptomeninges.
(D) Sections of an ex vivo culture of leptomeninges maintained in E2-EGF for 6 days. There are also CD11b+ (green)/Ki67+ (red) cells in an enlarged round piece of leptomeninges. Nuclei were stained blue by Hoechst 33258. Scale bar = 50 μm.
From: Tanaka J, Takahashi H, Yano H, Nakanishi H.
Generation of CSF1-Independent Ramified Microglia-Like Cells from Leptomeninges In Vitro..
Cells. 2020 Dec 25;10(1):E24.
doi: 10.3390/cells10010024.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify microglia in vitro by immunofluorescence.
Image caption:
Generation of Iba1+ ramified microglia-like cells from leptomeningeal cells on poly-L-lysine (PLL)-coated glass coverslips in Dulbecco’s modified Eagle′s medium containing 10% fetal calf serum (FCS-DMEM).
(F) Cells derived from the cultured tissue were fixed and immunostained with antibodies against CD11b (green) and GFAP (red). CD11b+ cells (green) showed only amoeboid morphology. Nuclei were stained blue by Hoechst 33258. Scale bar = 25 μm.
From: Tanaka J, Takahashi H, Yano H, Nakanishi H.
Generation of CSF1-Independent Ramified Microglia-Like Cells from Leptomeninges In Vitro..
Cells. 2020 Dec 25;10(1):E24.
doi: 10.3390/cells10010024.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to label microglia in rat brain by immunofluorescence.
Image caption:
Effects of stress and progesterone on HMGB1 distribution in activated microglia. Representative immunofluorescence merged images showing the distribution of HMGB1 (green) and CD11b positive microglia (red) in the hippocampal CA1 region at 7 and 14 days after global ischemia, or from sham animals. White arrows indicate HMGB1 located in the nuclei of surveillant microglia in sham animals; white arrowheads indicate HMGB1 translocated from the nucleus to the cytoplasm of activated microglia in ischemic animals. Right squares show enlargements of selected cells. so, stratum oriens.; sp., stratum pyramidale.; sr, stratum radiatum.. Scale bar: 50 μm.
From: Espinosa-Garcia C, Atif F, Yousuf S, Sayeed I, Neigh GN, Stein DG.
Progesterone Attenuates Stress-Induced NLRP3 Inflammasome Activation and Enhances Autophagy following Ischemic Brain Injury.
Int J Mol Sci. 2020 May 26;21(11):3740.
doi: 10.3390/ijms21113740.
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Mouse anti Rat CD11b/c antibody, clone OX-42 (MCA275R) used to label microglia in vitro following LPS treatment by immunofluorescence.
Image caption:
Confocal fluorescence imaging of MMP-9 in rat neonatal microglia treated for 18 h with either (A) E. coli LPS (1 ng/mL), or (B) P. gingivalis LPS (105 ng/mL). The subcellular and cellular markers are 4',6-diamidino-2-phenylindole (DAPI, blue), primary mouse anti-rat CD11b/c (green) and primary rabbit anti-rat MMP-9 (red). The scale bar denotes 10 μm.
From: Memedovski Z, Czerwonka E, Han J, Mayer J, Luce M, Klemm LC, Hall ML, Mayer AMS.
Classical and Alternative Activation of Rat Microglia Treated with Ultrapure Porphyromonas gingivalis Lipopolysaccharide In Vitro.
Toxins (Basel). 2020 May 19;12(5):333.
doi: 10.3390/toxins12050333.
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Mouse anti Rat CD11b/c antibody, clone OX-42 (MCA275R) used to label microglia in vitro. by immunofluorescence.
Image caption:
M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states.
From: Mecha M, Yanguas-Casás N, Feliú A, Mestre L, Carrillo-Salinas FJ, Riecken K, Gomez-Nicola D, Guaza C.
Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis.
J Neuroinflammation. 2020 Mar 19;17(1):88.
doi: 10.1186/s12974-020-01734-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275GA) used to label rat microglia by immunofluorescence.
Image caption:
Double immunofluorescence for OX-42 and β1-AR. Scale bar: 20 μm.
From: Sugama S, Takenouchi T, Hashimoto M, Ohata H, Takenaka Y, Kakinuma Y.
Stress-induced microglial activation occurs through β-adrenergic receptor: noradrenaline as a key neurotransmitter in microglial activation.
J Neuroinflammation. 2019 Dec 17;16(1):266.
doi: 10.1186/s12974-019-1632-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275G) used to assess inflammation levels in rat samples by western blotting.
Image caption:
Effect of pregnancy swimming on the pup's protein levels in right cortex extracts 24 h following HI (PND4). Bars graph represent immunoblots of (B) cell death (ccaspase 3 and fractin) and inflammation (CD11b) in the four experimental groups: sedentary sham (SESH), sedentary hypoxic-ischemic (SEHI), swimming sham (SWSH) and swimming hypoxic-ischemic (SWHI). WB results are plotted normalized to SESH level expression (100%) (mean ± SEM). Significance testing was determined using one-way ANOVA followed by Duncan's post hoc and was performed on Actin or βIII-tubulin normalized data. *HI vs. its respective SH group, Significance accepted when p <0.05.
From: Sanches EF, Van de Looij Y, Toulotte A, da Silva AR, Romero J, Sizonenko SV.
Brain Metabolism Alterations Induced by Pregnancy Swimming Decreases Neurological Impairments Following Neonatal Hypoxia-Ischemia in Very Immature Rats.
Front Neurol. 2018 Jun 25;9:480.
doi: 10.3389/fneur.2018.00480.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to label microglia in rat brain by immunofluorescence.
Image caption:
Immunomodulatory effect of chronic treatment with CBD on microglia activation and polarization in the SNc.
Representative images of the microglia activation and polarization in the SNc in both experimental groups. The results are expressed as mean ± SEM. N = 6 to 8 in each group.
From: Giuliano C, Francavilla M, Ongari G, Petese A, Ghezzi C, Rossini N, Blandini F, Cerri S.
Neuroprotective and Symptomatic Effects of Cannabidiol in an Animal Model of Parkinson's Disease.
Int J Mol Sci. 2021 Aug 18;22(16):8920.
doi: 10.3390/ijms22168920.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to label microglia in rat brain by immunofluorescence.
Image caption:
Immunomodulatory effect of chronic treatment with CBD on microglia activation and polarization in the SNc.
Representative images of the microglia activation and polarization in the SNc in both experimental groups. The results are expressed as mean ± SEM. N = 6 to 8 in each group.
From: Giuliano C, Francavilla M, Ongari G, Petese A, Ghezzi C, Rossini N, Blandini F, Cerri S.
Neuroprotective and Symptomatic Effects of Cannabidiol in an Animal Model of Parkinson's Disease.
Int J Mol Sci. 2021 Aug 18;22(16):8920.
doi: 10.3390/ijms22168920.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to label microglia in rat brain by immunofluorescence.
Image caption:
Microglia can be rapidly activated and peripheral leukocytes were recruited to the retina in EIU. A: One microgram of lipopolysaccharide (LPS) was injected into the vitreous of the 4Di-10ASP labeling rats to induce endotoxin-induced uveitis (EIU). The retinas were harvested and stained with a microglial marker CD11b (red) after infection for 3, 6, and 12 h. Representative immunofluorescence images of the retinal whole-mounts were obtained with confocal microscopy (n=6 per group). Scale bars: 40 μm.
From: Yuan Z, Chen X, Yang W, Lou B, Ye N, Liu Y.
The anti-inflammatory effect of minocycline on endotoxin-induced uveitis and retinal inflammation in rats.
Mol Vis. 2019 Jul 5;25:359-72.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to demonstrate microglia in rat brain by immunofluorescence on PFA fixed cryosections.
Image caption:
Effects of hypoxia and CsA treatment on glial cells. Immunohistochemical staining (GFAP and CD11b) of hypoxia- and CsA-treated retinas: representative staining of retinas cultured for 48 h. (A) Macroglia were labeled using a GFAP antibody (green) and cell nuclei labled with DAPI (purple); (B) microglia were stained with a CD11b antibody (green) and cell nuclei labeled with DAPI (purple). (C) The percentage of the GFAP+ area compared to the total area is presented in the diagram. Scale bar indicates 30 μm. The percentage of the macroglia area was higher than that of pure hypoxia at 6 μg/mL CsA. The higher concentration of 9 μg/mL CsA maintained GFAP abundance almost at the level of control retinas. (D) The percentage of microglia was higher in CsA-treated retinas (approximately 2.5% to 3.25%) than in controls (approximately 2%) and pure hypoxia (approximately 1.8%). Significances are indicated as * with respect to the control condition and as + with respect to hypoxia treatment using the following significance levels: + p <0.05, ** p <0.01, and *** p <0.001.
From: Schnichels S, Schultheiss M, Klemm P, Blak M, Herrmann T, Melchinger M, Bartz-Schmidt KU, Löscher M, Zeck G, Spitzer MS, Hurst J.
Cyclosporine A Protects Retinal Explants against Hypoxia
Int J Mol Sci. 2021 Sep 22;22(19):10196.
doi: 10.3390/ijms221910196.
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Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R used to identify microglia and infiltrating leukocytes in rat brain by immunofluorescence.
Image caption:
Immunofluorescent labeling of A1ARs (green), CD11b (red) and DAPI (blue) in the ischemic region, shown as merged channels and neurological outcome after ischemia. The data show the temporal evolution of A1ARs expression in microglia and infiltrated leukocytes (macrophages and neutrophils) expressing CD11b at day 0 (A), day 1 (B), day 3 (C) and day 7 (D) after ischemia.
From: Joya A, Ardaya M, Montilla A, Garbizu M, Plaza-García S, Gómez-Vallejo V, Padro D, Gutiérrez JJ, Rios X, Ramos-Cabrer P, Cossío U, Pulagam KR, Higuchi M, Domercq M, Cavaliere F, Matute C, Llop J, Martín A.
In vivo multimodal imaging of adenosine A1 receptors in neuroinflammation after experimental stroke.
Theranostics. 2021 Jan 1;11(1):410-425.
doi: 10.7150/thno.51046.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b, clone OX-42 (MCA275R) used to label microglia in rat astrocyte cultures by immunofluorescence.
Image caption:
Cultures stained for the astrocytic marker GFAP (red) and the microglial marker OX42 (green), upper figures.
Actin filament staining with Alexa488-conjugated phalloidin which shows mainly stress fibers in the control culture and after restoration while cells treated with LPS show a diffuse reorganization of the stress fibers, lower figures. Scale bar = 50μm. Representative images are presented.
From: Hansson E, Skiöldebrand E (2019)
Anti-inflammatory effects induced by ultralow concentrations of bupivacaine in combination with ultralow concentrations of sildenafil (Viagra) and vitamin D3 on inflammatory reactive brain astrocytes.
PLoS ONE 14(10): e0223648.
doi: 10.1371/journal.pone.0223648
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to stain microglia cells in rat brain sections by immunofluorescence.
Image caption:
Time course of microglia activation. (A) Double immunostaining for microglia, and TNFα in OHSCs. Panels e–h depict representative images showing immunopositive microglia-like cells at 3 (e), 7 (f), 14 (g), and 21 (h) DIV. Expression of TNFα (green) and double staining with CD11b (red) at different stages are shown in panels i–l and m–p, respectively. All images were taken in the CA1 region. Scale bar, 50.0μm
From: Chong SA, Balosso S, Vandenplas C, Szczesny G, Hanon E, Claes K, Van Damme X, Danis B, Van Eyll J, Wolff C, Vezzani A, Kaminski RM, Niespodziany I.
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.
Neurotherapeutics. 2018 Apr;15(2):470-488.
doi: 10.1007/s13311-018-0607-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275G) used to identify microglia in rat brain by immunohistochemistry on formalin fixed cryostat sections.
Image caption:
Immunohistochemical analysis of hESC-derived vmDA and vFB grafts. Activated microglia within the graft core and border (Ox42 [CD11b], Serotec, MCA275G, 1:1000) at 5× and 20× magnification in each graft group.
From: Lane EL, Harrison DJ, Ramos-Varas E, Hills R, Turner S, Lelos MJ.
Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease.
Mov Disord. 2021 Nov 12 [Epub ahead of print].
doi: 10.1002/mds.28856.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b, clone OX-42 (MCA275R) used to label microglia in rat brain by immunohistochemistry on formalin fixed cryostections.
Image caption:
Quantification of CD11b+ microglia and CD45+ leukocyte in the striatum of the grafted rats. (A,B) Bright field images showing CD11b+ cells density and morphology around the graft and their quantification respectively in the striatum of the rats received either saline, exendin-4 or liraglutide and ± LD. ** p <0.01, *** p <0.001, **** p <0.0001. Scale bar (A,C) = 100 μm.
From:Elabi OF, Davies JS, Lane EL.
L-dopa-Dependent Effects of GLP-1R Agonists on the Survival of Dopaminergic Cells Transplanted into a Rat Model of Parkinson Disease.
Int J Mol Sci. 2021 Nov 16;22(22):12346.
doi: 10.3390/ijms222212346.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275G) used to label microglia in rat brain by immunofluorescence.
Image caption:
Fluorescent labeling of astrocytes (immunostaining of glial fibrillary acidic protein, GFAP), microglia (immunostaining of CD11b and IL-1β), and cellular nuclei (DAPI staining, in blue) in the parietal cortex (Ctx) and dentate gyrus (DG) 24 h after the onset of pilocarpine-induced seizures lasting 3 h. Merge images show the colocalization of IL-1β immunosignal with microglial elements (yellow arrows) in both regions, whereas no IL-1β immunopositivity is found in the astrocytes. Two different sections of DG at different magnifications are shown.
From: Andrioli, A.; Fabene, P.F.; Mudò, G.; Barresi, V.; Di Liberto, V.; Frinchi, M.; Bentivoglio, M.; Condorelli, D.F.
Downregulation of the Astroglial Connexin Expression and Neurodegeneration after Pilocarpine-Induced Status Epilepticus.
Int. J. Mol. Sci. 2023, 24, 23.
doi: 0.3390/ijms24010023
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b, clone OX-42 (MCA275R) used to stain CD11+ve cells in rat brain by immunofluorescence.
Image caption:
Upregulation of IRAP expression in CD11b and GFAP positive cells following stroke. Representative immunofluorescent images demonstrating (A) upregulation of IRAP in activated CD11b positive cells within the stroked cortical core. The white scale bar represents 100 μm.
From:Telianidis J, Hunter A, Widdop R, Kemp-Harper B, Pham V, McCarthy C, Chai SY.
Inhibition of insulin-regulated aminopeptidase confers neuroprotection in a conscious model of ischemic stroke.
Sci Rep. 2023 Nov 13;13(1):19722.
doi: 10.1038/s41598-023-46072-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse ati Rat CD11b antibody, clone OX-42 (MCA275G) used to identify microglia in rat brain by immunofluorescence.
Image caption:
TAT-Src protects against neuronal loss in vivo following NMDA-mediated neurotoxicity.
B: Representative photomicrographs of FITC-labeled TAT-Src infusion co-labeled against markers of neuronal cells (NeuN+: red); astrocytes (GFAP+: red) and microglia (CD11b/c (Clone OX-42)+: red) and observed as orange.
From: Ameen SS, Griem-Krey N, Dufour A, Hossain MI, Hoque A, Sturgeon S, Nandurkar H, Draxler DF, Medcalf RL, Kamaruddin MA, Lucet IS, Leeming MG, Liu D, Dhillon A, Lim JP, Basheer F, Zhu HJ, Bokhari L, Roulston CL, Paradkar PN, Kleifeld O, Clarkson AN, Wellendorph P, Ciccotosto GD, Williamson NA, Ang CS, Cheng HC.
N-Terminomic Changes in Neurons During Excitotoxicity Reveal Proteolytic Events Associated With Synaptic Dysfunctions and Potential Targets for Neuroprotection.
Mol Cell Proteomics. 2023 May;22(5):100543.
doi: 10.1016/j.mcpro.2023.100543.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify microglia in rat brain by immunofluorescence.
Image caption:
TGF β activated microglia surface molecules alter OPC differentiation in vitro.(f) Representative images of cultured microglia (young and aged).
From: Baror R, Neumann B, Segel M, Chalut KJ, Fancy SPJ, Schafer DP, Franklin RJM.
Transforming growth factor-beta renders ageing microglia inhibitory to oligodendrocyte generation by CNS progenitors.
Glia. 2019 Jul;67(7):1374-1384.
doi: 10.1002/glia.23612.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b, clone OX-42 (MCA275R) used to assess CD11b expression in rat brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Immunohistochemical analysis of hESC-derived vmDA and vFB grafts. Activated microglia within the graft core and border (Ox42 [CD11b], Serotec, MCA275G, 1:1000) at 5× and 20× magnification in each graft group.
From: Lane EL, Harrison DJ, Ramos-Varas E, Hills R, Turner S, Lelos MJ.
Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease.
Mov Disord. 2022 Mar;37(3):613-619.
doi: 10.1002/mds.28856.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify activated micropglia in rat brain sections by immunofluorescence.
Image caption:
sEVs containing CCL2 from astrocytes aggravate microglial activation and neuronal apoptosis in vivo. {m} Immunofluorescence staining to detect the expression of the microglial activation marker OX42.
From: Rong Y, Ji C, Wang Z, Ge X, Wang J, Ye W, Tang P, Jiang D, Fan J, Yin G, Liu W, Cai W.
Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 2021 Sep 12;18(1):196.
doi: 10.1186/s12974-021-02268-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R) used to identify activated micropglia in rat brain sections by immunofluorescence.
Image caption:
The CCR2 antagonist INCB3344 decreases microglial activation and neuronal apoptosis after SCI. (i) Immunofluorescence staining to detect the expression of the microglial activation marker OX42.
From: Rong Y, Ji C, Wang Z, Ge X, Wang J, Ye W, Tang P, Jiang D, Fan J, Yin G, Liu W, Cai W.
Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 2021 Sep 12;18(1):196.
doi: 10.1186/s12974-021-02268-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD11b
- Product Type
- Monoclonal Antibody
- Clone
- OX-42
- Isotype
- IgG2a
- Specificity
- CD11b
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD11b, clone OX-42 recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinson et al.1986). Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinson et al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA275GA, MCA275G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA275R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Resident rat peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/50 | 1/100 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative /positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
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Log in | ||||||
| Description | Mouse IgG2a Negative Control | ||||||
References for CD11b antibody
-
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Jin, S.X. et al. (2003) p38 mitogen-activated protein kinase is activated after a spinal nerve ligation in spinal cord microglia and dorsal root ganglion neurons and contributes to the generation of neuropathic pain.
J Neurosci. 23: 4017-22.
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- Synonyms
- Integrin Alpha M Chain
- MAC-1
- RRID
- AB_321301
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