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CD11b antibody | ED7

Product Code Applications Pack Size List Price Your Price Qty
MCA618R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F FN * 0.25 mg loader
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loader

Mouse anti Rat CD11b antibody, clone ED7 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. Like ED8, ED7 recognises small ramified microglia in the central nervous system. No other cell types are positive for ED7, except for the cilia of the bronchus epithelium. The recognized antigen is a heterodimer (~160 and ~95 kDa) belonging to the family of adhesion molecules (CD11b/CD18). ED7 and ED8 may recognize closely related epitopes on the same molecule. ED7 and ED8 have been shown to induce homotypic aggregation of granulocytes.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat spleen cell homogenate.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 Ag-14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays 1
Immunohistology - Frozen 2 1/500
Immunohistology - Paraffin
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD11b antibody

  1. DeGroot, C.J. et al. (1988) Discrimination between different types of neuroglial cells in rat central nervous system using combined immuno- and enzyme-histochemical methods.
    Immunobiology. 178 (3): 177-90.
  2. Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
    J Leukoc Biol. 46 (6): 556-64.
  3. Damoiseaux, J.G. et al. (1989) Rat bone marrow and monocyte cultures: influence of culture time and lymphokines on the expression of macrophage differentiation antigens.
    J Leukoc Biol. 46 (3): 246-53.
  4. Huitinga, I. et al. (1993) Treatment with anti-CR3 antibodies ED7 and ED8 suppresses experimental allergic encephalomyelitis in Lewis rats.
    Eur J Immunol. 23 (3): 709-15.
  5. Drasković-pavlović, B. et al. (1999) Differential effects of anti-rat CD11b monoclonal antibodies on granulocyte adhesiveness.
    Immunology. 96 (1): 83-9.
  6. Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
    Invest Ophthalmol Vis Sci. 42 (1): 170-6.
  7. Garn, H. et al. (2006) Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats.
    Respir Res. 7: 4.
  8. Ramaglia, V. et al. (2007) The membrane attack complex of the complement system is essential for rapid Wallerian degeneration.
    J Neurosci. 27 (29): 7663-72.
  9. View The Latest Product References

Functional Assays

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_322901

MCA618R

162575 1807

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