CD106 antibody | MR106
Mouse anti Rat CD106:Alexa Fluor® 647
Mouse anti Rat CD106:FITC
Mouse anti Rat CD106
- Product Type
- Monoclonal Antibody
- Clone
- MR106
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA4633A647T | F | 25 Tests |
|
|
| MCA4633FT | F | 25 µg |
|
|
| MCA4633F | F | 0.1 mg |
|
|
| MCA4633GA | C F IF | 0.1 mg |
|
Mouse anti Rat CD106 antibody, clone MR106 recognizes rat CD106, otherwise known as VCAM-1 (vascular adhesion molecule 1), a 110kDa inducible type I transmembrane glycoprotein and member of the immunoglobulin supergene family, which is predominantly expressed on vascular endothelium, and also on bone marrow stromal cells, follicular dendritic cells and some macrophages.
CD106 interacts with the lymphocyte homing receptor VLA-4 (alpha4 beta1 integrin) and LPAM-1 (alpha4 beta7 integrin) and mediates leucocyte-endothelial cell adhesion and signal transduction. Expression of CD106 is upregulated following cytokine activation, and endothelial CD106 plays a role in the extravasation of leucocytes from blood vessels during inflammation.
CD106 interacts with the lymphocyte homing receptor VLA-4 (alpha4 beta1 integrin) and LPAM-1 (alpha4 beta7 integrin) and mediates leucocyte-endothelial cell adhesion and signal transduction. Expression of CD106 is upregulated following cytokine activation, and endothelial CD106 plays a role in the extravasation of leucocytes from blood vessels during inflammation.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Rat CD106-transfected L5178Y cells.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P29534
- Entrez Gene
- Vcam1
- GO Terms
- GO:0001666 response to hypoxia
- GO:0002526 acute inflammatory response
- GO:0002544 chronic inflammatory response
- GO:0016021 integral to membrane
- GO:0005178 integrin binding
- GO:0007568 aging
- GO:0007584 response to nutrient
- GO:0010212 response to ionizing radiation
- GO:0042383 sarcolemma
- GO:0044419 interspecies interaction between organisms
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD106 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/25 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Product Specific References
References for CD106 antibody
-
Miyao, N. et al. (2006) Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury.
Am J Physiol Lung Cell Mol Physiol. 290 (6): L1059-68. -
Kubota, H. et al. (2007) Identification and characterization of vitamin A-storing cells in fetal liver: implications for functional importance of hepatic stellate cells in liver development and hematopoiesis.
Stem Cells. 25 (9): 2339-49.
Further Reading
-
Bevilacqua, M.P. (1993) Endothelial-leukocyte adhesion molecules.
Annu Rev Immunol. 11: 767-804.
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