CD106 antibody | MR106

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Anti Rat CD106 Antibody, clone MR106 thumbnail image 1

Anti Rat CD106 Antibody, clone MR106 thumbnail image 2

Anti Rat CD106 Antibody, clone MR106 thumbnail image 3

Anti Rat CD106 Antibody, clone MR106 thumbnail image 4

Anti Rat CD106 Antibody, clone MR106 thumbnail image 5

Anti Rat CD106 Antibody, clone MR106 gallery image 1 — Staining of rat bone marrow cells with Mouse anti Rat CD106:FITC (**MCA4633F**)

Anti Rat CD106 Antibody, clone MR106 gallery image 2 — Staining of rat bone marrow cells with Mouse anti Rat CD106:Alexa Fluor^® 647 (**MCA4633A647**)

Anti Rat CD106 Antibody, clone MR106 gallery image 3 — Staining of rat bone marrow cells with Mouse anti Rat CD106:RPE (**MCA4633PE**)

Anti Rat CD106 Antibody, clone MR106 gallery image 4 — Figure A. FITC conjugated mouse anti rat CD11b (MCA619F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti rat CD11b (MCA619F) and RPE conjugated mouse anti rat CD106 (**MCA4633PE**). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.

Anti Rat CD106 Antibody, clone MR106 gallery image 5 — Figure A. PE conjugated mouse anti rat CD106 (**MCA4633PE**) and FITC conjugated mouse IgG2a isotype control (MCA1210FITC). Figure B. PE conjugated mouse anti rat CD106 (**MCA4633PE**) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.

Mouse anti Rat CD106:Alexa Fluor® 488

Mouse anti Rat CD106:Alexa Fluor® 647

Mouse anti Rat CD106:FITC

Mouse anti Rat CD106

Mouse anti Rat CD106:RPE

Product Type: Monoclonal Antibody
Clone: MR106
Isotype: IgG1

Product Code	Applications	Pack Size
MCA4633A488T	F	25 Tests
MCA4633A488	F	100 Tests/1ml
MCA4633A647T	F	25 Tests
MCA4633A647	F	100 Tests/1ml
MCA4633FT	F	25 µg
MCA4633F	F	0.1 mg
MCA4633GA	C F IF	0.1 mg
MCA4633PE	F	100 Tests

Mouse anti Rat CD106 antibody, clone MR106 recognizes rat CD106, otherwise known as VCAM-1 (vascular adhesion molecule 1), a 110kDa inducible type I transmembrane glycoprotein and member of the immunoglobulin supergene family, which is predominantly expressed on vascular endothelium, and also on bone marrow stromal cells, follicular dendritic cells and some macrophages.

CD106 interacts with the lymphocyte homing receptor VLA-4 (alpha4 beta1 integrin) and LPAM-1 (alpha4 beta7 integrin) and mediates leucocyte-endothelial cell adhesion and signal transduction. Expression of CD106 is upregulated following cytokine activation, and endothelial CD106 plays a role in the extravasation of leucocytes from blood vessels during inflammation.

Product Details

Target Species: Rat
Product Form: Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form: Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: Rat CD106-transfected L5178Y cells.
Approx. Protein Concentrations: IgG concentration 0.05mg/ml
Approx. Protein Concentrations: IgG concentration 0.05mg/ml
Approx. Protein Concentrations: IgG concentration 0.1mg/ml
Approx. Protein Concentrations: IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P29534 Related reagents
Entrez Gene: Vcam1 Related reagents
GO Terms: GO:0001666 response to hypoxia; GO:0002526 acute inflammatory response; GO:0002544 chronic inflammatory response; GO:0016021 integral to membrane; GO:0005178 integrin binding; GO:0007568 aging; GO:0007584 response to nutrient; GO:0010212 response to ionizing radiation; GO:0042383 sarcolemma; GO:0044419 interspecies interaction between organisms
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD106 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry		Neat
Flow Cytometry	Neat	1/5
Flow Cytometry	Neat	1/25
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 488	MCA1209A488	100 Tests/1ml	F
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA1209A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA1209F	0.1 mg	F
Mouse IgG1 Negative Control	MCA1209	0.1 mg	F
Mouse IgG1 Negative Control:RPE	MCA1209PE	100 Tests	F

Product Specific References

References for CD106 antibody

Miyao, N. et al. (2006) Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury.
Am J Physiol Lung Cell Mol Physiol. 290 (6): L1059-68.
Kubota, H. et al. (2007) Identification and characterization of vitamin A-storing cells in fetal liver: implications for functional importance of hepatic stellate cells in liver development and hematopoiesis.
Stem Cells. 25 (9): 2339-49.