Aquaporin 1 antibody | 7D11

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Mouse anti Aquaporin 1

Product Type
Monoclonal Antibody
Clone
7D11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2100 E F P* WB 0.1 mg
Mouse anti Aquaporin 1 antibody, clone 7D11 recognizes an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28)

Aquaporin 1 is a 28kDa integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kDa in mass.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
0.1%Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to amino acids 198-208 of rat aquaporin 1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended.
Shelf Life
18 months from date of despatch

More Information

UniProt
P29975 Related reagents
Entrez Gene
Aqp1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005903 brush border
GO:0010592 positive regulation of lamellipodium assembly
GO:0010634 positive regulation of epithelial cell migration
GO:0015168 glycerol transmembrane transporter activity
GO:0015250 water channel activity
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0021670 lateral ventricle development
GO:0032809 neuronal cell body membrane
GO:0042538 hyperosmotic salinity response
GO:0043627 response to estrogen stimulus
GO:0043679 axon terminus
Regulatory
For research purposes only

Applications of Aquaporin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Flow Cytometry
Immunohistology - Paraffin 1
Western Blotting 1/1000 1/2000
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA1209 0.1 mg F

Product Specific References

References for Aquaporin 1 antibody

  1. Nagy G et al. (2002) Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
    Pathol Oncol Res. 8 (2): 115-24.
  2. Bódis, B. et al. (2001) Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
    J Physiol Paris. 95 (1-6): 271-5.
  3. Felszeghy, S. et al. (2004) Expression of aquaporin isoforms during human and mouse tooth development.
    Arch Oral Biol. 49: 247-57.
  4. Cabral, P.D and Herrera, M. (2012) Membrane-associated aquaporin-1 facilitates osmotically driven water flux across the basolateral membrane of the thick ascending limb.
    Am J Physiol Renal Physiol. 303: F621-9.