Aquaporin 1 antibody | 7D11

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Mouse anti Aquaporin 1

Product Type: Monoclonal Antibody
Clone: 7D11
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2100	E F P* WB			0.1 mg

Mouse anti Aquaporin 1 antibody, clone 7D11 recognizes an epitope within an extracellular region of the water-specific channel aquaporin 1 (also known as AQP1 or CHIP-28)

Aquaporin 1 is a 28kDa integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kDa in mass.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide
0.1%	Bovine Serum Albumin

Immunogen

Synthetic peptide corresponding to amino acids 198-208 of rat aquaporin 1.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended.
Shelf Life: 18 months from date of despatch

More Information

UniProt: P29975 Related reagents
Entrez Gene: Aqp1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0016021 integral to membrane; GO:0005903 brush border; GO:0010592 positive regulation of lamellipodium assembly; GO:0010634 positive regulation of epithelial cell migration; GO:0015168 glycerol transmembrane transporter activity; GO:0015250 water channel activity; GO:0016323 basolateral plasma membrane; GO:0016324 apical plasma membrane; GO:0021670 lateral ventricle development; GO:0032809 neuronal cell body membrane; GO:0042538 hyperosmotic salinity response; GO:0043627 response to estrogen stimulus; GO:0043679 axon terminus
Regulatory: For research purposes only

Applications of Aquaporin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/1000	1/10000
Flow Cytometry
Immunohistology - Paraffin ¹
Western Blotting	1/1000	1/2000

¹ This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA1209	0.1 mg	F

Product Specific References

References for Aquaporin 1 antibody

Nagy G et al. (2002) Development and characterisation of a monoclonal antibody family against aquaporin 1 (AQP1) and aquaporin 4 (AQP4).
Pathol Oncol Res. 8 (2): 115-24.
Bódis, B. et al. (2001) Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats.
J Physiol Paris. 95 (1-6): 271-5.
Felszeghy, S. et al. (2004) Expression of aquaporin isoforms during human and mouse tooth development.
Arch Oral Biol. 49: 247-57.
Cabral, P.D and Herrera, M. (2012) Membrane-associated aquaporin-1 facilitates osmotically driven water flux across the basolateral membrane of the thick ascending limb.
Am J Physiol Renal Physiol. 303: F621-9.

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