GAPDH antibody | 6C5
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Mouse anti Rabbit GAPDH
- Product Type
- Monoclonal Antibody
|Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human Pig Dog Cat Rat Mouse Xenopus Tube-nosed Bat Chicken Vertebrates Expected from Sequence Sheep African green monkey Crucian Carp
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Rabbit muscle GAPDH.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
References for GAPDH antibody
Latasa, M.U. et al. (2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.
PLoS One. 5: e15690.
Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
Drug Metab Dispos. 40 (8): 1603-10.
Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
J Biol Chem. 287: 16849-59.
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60.
Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
J Cell Biochem. 114 (6): 1355-63.
Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
J Allergy Clin Immunol. 124: 551-7.
Suzuki, K. et al. (2016) Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation.
J Immunol. 196 (3): 1338-47.
Beaudin, S. & Welsh, J. (2016) 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells.
Mol Cell Endocrinol. 424: 34-41.
View The Latest Product References
Hao, F. et al. (2017) Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
Cell Death Dis. 8 (10): e3152.
Wang, S. et al. (2019) Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse.
Biomaterials. 219: 119375.
Chen, C. et al. (2021) Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease
Cancers. 14 (1): 78.
Hihara, F. et al. (2022) In Vitro. Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study.
Int J Mol Sci. 23 (10): 5807.
- GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
- Entrez Gene
- GO Terms
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0051287 NAD binding
- View More GO Terms
- GO:0051402 neuron apoptosis
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0006094 gluconeogenesis
- GO:0048471 perinuclear region of cytoplasm
- GO:0005625 soluble fraction
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