GAPDH antibody | 6C5

Mouse anti Rabbit GAPDH

Product Type
Monoclonal Antibody
Clone
6C5
Isotype
IgG1
Specificity
GAPDH

Product Code Applications Pack Size List Price Your Price Qty
MCA4739
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF IP WB 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.

GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).

Target Species
Rabbit
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Pig
Dog
Cat
Rat
Mouse
Xenopus
Tube-nosed Bat
Chicken
Vertebrates Expected from Sequence
Sheep
African green monkey
Crucian Carp
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Rabbit muscle GAPDH.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for GAPDH antibody

  1. Latasa, M.U. et al. (2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.
    PLoS One. 5: e15690.
  2. Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
    Drug Metab Dispos. 40 (8): 1603-10.
  3. Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
    J Biol Chem. 287: 16849-59.
  4. Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
    BMC Immunol. 13: 60.
  5. Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
    J Cell Biochem. 114 (6): 1355-63.
  6. Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
    J Allergy Clin Immunol. 124: 551-7.
  7. Suzuki, K. et al. (2016) Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation.
    J Immunol. 196 (3): 1338-47.
  8. Beaudin, S. & Welsh, J. (2016) 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells.
    Mol Cell Endocrinol. 424: 34-41.
  9. View The Latest Product References
  10. Hao, F. et al. (2017) Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
    Cell Death Dis. 8 (10): e3152.
  11. Wang, S. et al. (2019) Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse.
    Biomaterials. 219: 119375.
  12. Chen, C. et al. (2021) Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease
    Cancers. 14 (1): 78.
  13. Hihara, F. et al. (2022) In Vitro. Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study.
    Int J Mol Sci. 23 (10): 5807.

Western Blotting

Synonyms
GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
RRID
AB_1720065
UniProt
P46406
P04406
P04797
P16858
P00355
Entrez Gene
GAPDH
GAPDH
Gapdh
Gapdh
GAPDH
GO Terms
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0051287 NAD binding
GO:0051402 neuron apoptosis
GO:0005515 protein binding
GO:0016020 membrane
GO:0006094 gluconeogenesis
GO:0048471 perinuclear region of cytoplasm
GO:0005625 soluble fraction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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