GAPDH antibody | 6C5
8.75 μl of each IP was loaded onto an AnykD™ Criterion™ TGX Stain-Free™ gel. 8.75 μl Jurkat whole cell lysate (WCL) was run in lanes 5 & 11 while Precision Plus Protein™ Prestained Standards were run in lane 6.
Rabbit anti GAPDH antibody (VPA00187) was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot™ (STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).
Mouse anti Rabbit GAPDH antibody, clone 6C5 (MCA4739) used as a western blotting loading control on mouse tissue lysates.
Image caption:
Loss of Caspase-8 enforces intrinsic apoptosis signaling after EtOH feeding.
(a) Left: Immunoblot analysis of CYP2E1 in the liver of WT and Casp8Δhepa mice kept on Lieber-DeCarli diet for 8 weeks. Right: Rationale for the subsequent measurements. We hypothesized that ethanol-driven apoptosis in Casp8Δhepa mice could involve de-regulated ceramide biosynthesis and enhanced cytochrome c release from mitochondria thereby inducing increased Caspase-9-mediated intrinsic apoptosis. Accordingly, the indicated intermediate steps were systematically investigated by immunoblot analysis, immunofluorescence stainings and qPCR. (b) Western blot analysis of cytochrome c levels in cytosolic and mitochondrial liver fractions. (c) Immunoblot analysis of cleaved Caspase-9 in the liver of WT and Casp8Δhepa mice. Quantification of Western blots was performed by densitometry.
From: Hao F, Cubero FJ, Ramadori P, Liao L, Haas U, Lambertz D, Sonntag R, Bangen JM, Gassler N, Hoss M, Streetz KL, Reissing J, Zimmermann HW, Trautwein C, Liedtke C, Nevzorova YA.
Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
Cell Death Dis. 2017 Oct 26;8(10):e3152.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit GAPDH:HRP
Mouse anti Rabbit GAPDH
Mouse anti Rabbit GAPDH:DyLight®800
Mouse anti Rabbit GAPDH:DyLight®680
- Product Type
- Monoclonal Antibody
- Clone
- 6C5
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA4739P | C E WB | 0.1 mg |
|
|
| MCA4739 | C E IF IP WB | 0.2 mg |
|
|
| MCA4739D800 | WB | 0.1 mg |
|
|
| MCA4739D680 | WB | 0.1 mg |
|
Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).
Product Details
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Pig Dog Cat Rat Mouse Xenopus Tube-nosed Bat Chicken Vertebrates Expected from Sequence Sheep African green monkey Crucian Carp - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to DyLight®800 - liquid
- Product Form
- Purified IgG conjugated to DyLight®680 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP stabiliser - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Rabbit muscle GAPDH.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46406
- P04406
- P04797
- P16858
- P00355
- Entrez Gene
- GAPDH
- GAPDH
- Gapdh
- Gapdh
- GAPDH
- GO Terms
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0006094 gluconeogenesis
- GO:0048471 perinuclear region of cytoplasm
- GO:0005625 soluble fraction
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
- Regulatory
- For research purposes only
Applications of GAPDH antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen | |||
| Western Blotting | 1/1000 | 1/5000 | |
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Western Blotting | 1/1000 | 1/10000 | |
| Western Blotting | 1/1000 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA4739P is suitable for use as a loading control
- Western Blotting
- MCA4739D800 is suitable for use as a loading control
- Western Blotting
- MCA4739D680 is suitable for use as a loading control
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for GAPDH antibody
-
Latasa, M.U. et al. (2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.
PLoS One. 5: e15690. -
Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
Drug Metab Dispos. 40 (8): 1603-10. -
Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
J Biol Chem. 287: 16849-59. -
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60. -
Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
J Cell Biochem. 114 (6): 1355-63. -
Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
J Allergy Clin Immunol. 124: 551-7. -
Suzuki, K. et al. (2016) Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation.
J Immunol. 196 (3): 1338-47. -
Beaudin, S. & Welsh, J. (2016) 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells.
Mol Cell Endocrinol. 424: 34-41. -
Hao, F. et al. (2017) Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
Cell Death Dis. 8 (10): e3152. -
Wang, S. et al. (2019) Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse.
Biomaterials. 219: 119375.
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