GAPDH antibody | 6C5
8.75 μl of each IP was loaded onto an AnykD Criterion TGX Stain-Free gel. 8.75 μl Jurkat whole cell lysate (WCL) was run in lanes 5 & 11 while Precision Plus Protein™ Prestained Standards were run in lane 6.
Rabbit anti GAPDH antibody (VPA00187) was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot™ (STAR209P) was used at 1/200 and visualized on the Bio-Rad Chemidoc Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).
Mouse anti Rabbit GAPDH antibody, clone 6C5 (MCA4739) used as a western blotting loading control on mouse tissue lysates.
Image caption:
Loss of Caspase-8 enforces intrinsic apoptosis signaling after EtOH feeding.
(a) Left: Immunoblot analysis of CYP2E1 in the liver of WT and Casp8Δhepa mice kept on Lieber-DeCarli diet for 8 weeks. Right: Rationale for the subsequent measurements. We hypothesized that ethanol-driven apoptosis in Casp8Δhepa mice could involve de-regulated ceramide biosynthesis and enhanced cytochrome c release from mitochondria thereby inducing increased Caspase-9-mediated intrinsic apoptosis. Accordingly, the indicated intermediate steps were systematically investigated by immunoblot analysis, immunofluorescence stainings and qPCR. (b) Western blot analysis of cytochrome c levels in cytosolic and mitochondrial liver fractions. (c) Immunoblot analysis of cleaved Caspase-9 in the liver of WT and Casp8Δhepa mice. Quantification of Western blots was performed by densitometry.
From: Hao F, Cubero FJ, Ramadori P, Liao L, Haas U, Lambertz D, Sonntag R, Bangen JM, Gassler N, Hoss M, Streetz KL, Reissing J, Zimmermann HW, Trautwein C, Liedtke C, Nevzorova YA.
Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
Cell Death Dis. 2017 Oct 26;8(10):e3152.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rabbit GAPDH (MCA4739) used as a Western blotting loading control on mouse liver lysates.
Image caption:
Fibropolycystic disease in aging JnkΔhepa mice is characterized by extracellular matrix deposition and inflammation.
Western Blot of 32 week Jnkf/f and Jnkδhepa mouse liver lysates for αSMA with GAPDH used as a loading control.
From: Chen C, Wu H, Ye H, Tortajada A, Rodríguez-Perales S, Torres-Ruiz R, Vidal A, Peligros MI, Reissing J, Bruns T, Mohamed MR, Zheng K, Lujambio A, Iraburu MJ, Colyn L, Latasa MU, Arechederra M, Fernández-Barrena MG, Berasain C, Vaquero J, Bañares R, Nelson LJ, Trautwein C, Davis RJ, Martinez-Naves E, Nevzorova YA, Villanueva A, Avila MA, Cubero FJ.
Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease.
Cancers. 2022; 14(1):78.
doi: 10.3390/cancers14010078
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit GAPDH (MCA4739) used as a Western blotting loading control on mouse liver lysates.
Image caption:
Activation of the unfolded protein response (UPR) triggers increased liver injury in Jnk∆hepa mice. (A) The expression of BiP/GRP78, CHOP, spliced XBP1 (sXBP1) and unspliced XBP1 (uXBP1) was evaluated in 32-week-old Jnkf/f and Jnk∆hepa mice using Western blot. (B) The expression of the same proteins was measured in Tunicamycin (TM)-challenged Jnkf/f and JnkΔhepa mice. Numbers denote molecular weight (KDa) of proteins. GAPDH served as loading control. #/* p <0.05 intra- and intergroup significance, respectively.
From: Chen C, Wu H, Ye H, Tortajada A, Rodríguez-Perales S, Torres-Ruiz R, Vidal A, Peligros MI, Reissing J, Bruns T, Mohamed MR, Zheng K, Lujambio A, Iraburu MJ, Colyn L, Latasa MU, Arechederra M, Fernández-Barrena MG, Berasain C, Vaquero J, Bañares R, Nelson LJ, Trautwein C, Davis RJ, Martinez-Naves E, Nevzorova YA, Villanueva A, Avila MA, Cubero FJ.
Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease.
Cancers. 2022; 14(1):78.
doi: 10.3390/cancers14010078
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit GAPDH antibody, clone 6C5 (MCA4739) used as a loading control for the western blot analysis of human cell line lysates.
Image caption:
Relationships between Western blots for EGFR expression and in vitro cell-binding assay with 64Cu-anti-EGFR antibodies (cetuximab). (A) Representative images of Western blots for EGFR and GAPDH expression.
From: Hihara F, Matsumoto H, Yoshimoto M, Masuko T, Endo Y, Igarashi C, Tachibana T, Shinada M, Zhang MR, Kurosawa G, Sugyo A, Tsuji AB, Higashi T, Kurihara H, Ueno M, Yoshii Y.
In Vitro Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study.
Int J Mol Sci. 2022 May 22;23(10):5807.
doi: 10.3390/ijms23105807.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit GAPDH antibody, clone 6C5 (MCA4739) used as a loading control to assess G6PD expression in tumor tissue lysates by western blotting.
Image caption:
G6PD protein levels are significantly increased in the tumor tissue isolated from the livers of HCC patients. (A) Total proteins were isolated from the livers tissue of HCC patients, and the Western blot analysis for EPSL15L1 and G6PD was carried out in the tumor (HCC, n = 7) and in the non-tumor (NTL, n = 7) cells.
From: Paluschinski M, Schira-Heinen J, Pellegrino R, Heij LR, Bednarsch J, Neumann UP, Longerich T, Stuehler K, Luedde T, Castoldi M.
Uncovering Novel Roles of miR-122 in the Pathophysiology of the Liver: Potential Interaction with NRF1 and E2F4 Signaling.
Cancers (Basel). 2023 Aug 16;15(16):4129.
doi: 10.3390/cancers15164129.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human GAPDH antibody, clone 6C5 (MCA4739) used as a loading control in western blotting evaluation of GDM exposure and in vitro hyperglycemia on ABR and PXN expression.
Image caption:
Effect of GDM exposure and in vitro hyperglycaemia on ABR and PXN protein
Immunoblot analysis of ABR and PXN after exposure to GDM (A and C) and after exposure to hyperglycaemia (B and D). Exposure to GDM and hyperglycaemia increased ABR protein, but not PXN. Representative immunoblots are shown above the graphs. For A, n = 6 control and n = 5 GDM and for C, n = 6 control and n = 4 GDM fpEC isolations were used. For B and D, n = 4 isolations grown under normoglycaemia (5.5 mM glucose) and hyperglycaemia (25 mM glucose) for 24 h were used. Significance was tested by an unpaired (A and C) and paired (B and D) two-tailed Student's t test.
From: Tokic S, Novakovic B, Leopold-Posch B, Mühlberger M, Hoch D, Lögl J, Bernhart EM, Saffery R, Desoye G, Hiden U.
The RhoA guanine exchange factor ABR: a glucose-sensitive mediator of actin reorganization in feto-placental arterial endothelial cells altered by gestational diabetes mellitus.
J Physiol. 2024 May 22. Epub ahead of print.
doi: 10.1113/JP285910.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rabbit GAPDH antibody, clone 6C5 (MCA4739) used as a loading control for the western blotting analysis of murine liver lysates in models of dietary induced steatohepatitis.
Image caption:
Weaker onset of fibrosis in CD62L−/− mice in two models of dietary induced steatohepatitis. (C) α-SMA Western blot analysis from liver extracts of WT and CD62L−/− animals. (D) Collagen 1α1 Western blot analysis of liver extracts of chow and HFD fed WT and CD62L−/− animals.
From: Drescher HK, Schippers A, Rosenhain S, Gremse F, Bongiovanni L, Bruin A, Eswaran S, Gallage SU, Pfister D, Szydlowska M, Heikenwalder M, Weiskirchen S, Wagner N, Trautwein C, Weiskirchen R, Kroy DC.
L-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men.
Cells. 2020 Apr 29;9(5):1106.
doi: 10.3390/cells9051106.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB IP ResetMouse anti Rabbit GAPDH
- Product Type
- Monoclonal Antibody
- Clone
- 6C5
- Isotype
- IgG1
- Specificity
- GAPDH
Antibody Quality and Performance Guarantee
| Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006). |
- Target Species
- Rabbit
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Pig Dog Cat Rat Mouse Xenopus Tube-nosed Bat Chicken Vertebrates Expected from Sequence Sheep African green monkey Crucian Carp - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Rabbit muscle GAPDH.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for GAPDH antibody
-
Koetzler, R. et al. (2009) Nitric oxide inhibits IFN regulatory factor 1 and nuclear factor-kappaB pathways in rhinovirus-infected epithelial cells.
J Allergy Clin Immunol. 124: 551-7. -
Latasa, M.U. et al. (2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.
PLoS One. 5: e15690. -
Zschemisch, N.H. et al. (2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 13: 60. -
Zizza, P. et al. (2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.
J Biol Chem. 287: 16849-59. -
Haller, S. et al. (2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.
Drug Metab Dispos. 40 (8): 1603-10. -
Agarwal, P. et al. (2013) Tumor suppressor gene p16/INK4A/CDKN2A-dependent regulation into and out of the cell cycle in a spontaneous canine model of breast cancer.
J Cell Biochem. 114 (6): 1355-63. -
Beaudin, S. & Welsh, J. (2016) 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells.
Mol Cell Endocrinol. 424: 34-41. -
Suzuki, K. et al. (2016) Human Host Defense Cathelicidin Peptide LL-37 Enhances the Lipopolysaccharide Uptake by Liver Sinusoidal Endothelial Cells without Cell Activation.
J Immunol. 196 (3): 1338-47.
View The Latest Product References
-
Hao, F. et al. (2017) Inhibition of Caspase-8 does not protect from alcohol-induced liver apoptosis but alleviates alcoholic hepatic steatosis in mice.
Cell Death Dis. 8 (10): e3152. -
Wang, S. et al. (2019) Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse.
Biomaterials. 219: 119375. -
Chen, C. et al. (2021) Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease
Cancers. 14 (1): 78. -
Hihara, F. et al. (2022) In Vitro. Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study.
Int J Mol Sci. 23 (10): 5807. -
Vucur, M. et al. (2023) Sublethal necroptosis signaling promotes inflammation and liver cancer.
Immunity. 56 (7): 1578-95.e8. -
Kim, J. et al. (2023) Targeted Deletion of Thymosin Beta 4 in Hepatic Stellate Cells Ameliorates Liver Fibrosis in a Transgenic Mouse Model.
Cells. 12 (12): 1658. -
Paluschinski, M. et al. (2023) Uncovering Novel Roles of miR-122 in the Pathophysiology of the Liver: Potential Interaction with NRF1 and E2F4 Signaling.
Cancers (Basel). 15 (16): 4129. -
Chen, C. et al. (2021) Platelet glycoprotein VI-dependent thrombus stabilization is essential for the intraportal engraftment of pancreatic islets.
Am J Transplant. 21 (6): 2079-89. -
Tokic, S. et al. (2024) The RhoA guanine exchange factor ABR: a glucose-sensitive mediator of actin reorganization in feto-placental arterial endothelial cells altered by gestational diabetes mellitus.
J Physiol. May 22 [Epub ahead of print]. -
Drescher, H.K. et al. (2020) L-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men.
Cells.9 (5): 1106. -
Guedes, J.P. et al. (2024) NatB Protects Procaspase-8 from UBR4-Mediated Degradation and Is Required for Full Induction of the Extrinsic Apoptosis Pathway.
Mol Cell Biol. : 1-14 [Epub ahead of Print]. -
Kim, J. et al. (2021) sEVs from tonsil-derived mesenchymal stromal cells alleviate activation of hepatic stellate cells and liver fibrosis through miR-486-5p.
Mol Ther. 29 (4): 1471-86.
- Synonyms
- GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
- RRID
- AB_1720065
- UniProt
- P46406
- P04406
- P04797
- P16858
- P00355
- Entrez Gene
- GAPDH
- GAPDH
- Gapdh
- Gapdh
- GAPDH
- GO Terms
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0051287 NAD binding
- View More GO Terms
- GO:0051402 neuron apoptosis
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0006094 gluconeogenesis
- GO:0048471 perinuclear region of cytoplasm
- GO:0005625 soluble fraction
View more products with GAPDH specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rabbit ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up