Progesterone antibody | 9B4

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Anti Progesterone Antibody, clone 9B4 thumbnail image 1

Anti Progesterone Antibody, clone 9B4 thumbnail image 2

Anti Progesterone Antibody, clone 9B4 gallery image 1 — Immunoperoxidase staining of paraffin embedded human ovary tissue with Mouse anti Progesterone antibody (**7720-1704**)

Anti Progesterone Antibody, clone 9B4 gallery image 2 — Immunoperoxidase staining of paraffin embedded human ovary tissue with Mouse anti Progesterone antibody (**7720-1704**)

Mouse anti Progesterone

Product Type: Monoclonal Antibody
Clone: 9B4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
7720-1704	E P*			1 mg

Mouse anti progesterone antibody, clone 9B4 is specific for progesterone, a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland.

Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.

Mouse anti progesterone antibody, clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone.

Product Details

Target Species

Broad

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Progesterone conjugated to bovine serum albumin at position 3.

Approx. Protein Concentrations

0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of Progesterone antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Paraffin ¹			1/50

¹ Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Histology Positive Control Tissue: Breast carcinoma and normal young ovarian tissue.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Progesterone antibody

Cahill, D.J. et al. (2003) Pre-ovulatory granulosa cells of infertile women with endometriosis are less sensitive to luteinizing hormone.
Am J Reprod Immunol. 49 (2): 66-9.
Alexandre-Pires, G. et al. (2014) Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes.
Microsc Res Tech. 77 (4): 313-25.
Daems, D. et al. (2017) Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor.
Anal Chim Acta. 950: 1-6.

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