Progesterone antibody | 9B4

Mouse anti Progesterone

Product Type
Monoclonal Antibody
Clone
9B4
Isotype
IgG1
Specificity
Progesterone

Product Code Applications Pack Size List Price Your Price Qty
7720-1704
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti progesterone antibody, clone 9B4 is specific for progesterone, a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland.

Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.

Mouse anti progesterone antibody, clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone.

Target Species
Broad
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Progesterone conjugated to bovine serum albumin at position 3.
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 1/50
  1. 1 Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma and normal young ovarian tissue.

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References for Progesterone antibody

  1. Cahill, D.J. et al. (2003) Pre-ovulatory granulosa cells of infertile women with endometriosis are less sensitive to luteinizing hormone.
    Am J Reprod Immunol. 49 (2): 66-9.
  2. Alexandre-Pires, G. et al. (2014) Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes.
    Microsc Res Tech. 77 (4): 313-25.
  3. Daems, D. et al. (2017) Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor.
    Anal Chim Acta. 950: 1-6.
  4. Nguyen, T.M.D. et al. (2019) Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation.
    PLoS One. 14 (6): e0217519.

RRID
AB_620620
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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