Progesterone antibody | 9B4
Mouse anti progesterone antibody, clone 9B4 (7720-1704) used to evaluate progesterone production by MLTC-1, Leydig tumor cells by ELISA.
Image caption:
Effect of fluoxetine in hLH or FSK/sub-hLH -promoted steroid production in MLTC-1 cells.
(A), (B) MLTC-1 cells were preincubated with or without FLX (or MET, or A-769662) for 1 hour and then the cells were stimulated for 3 hours with hLH or FSK/sub-hLH before progesterone productions were measured. Data are means ± SEM of 3 independent experiments performed in duplicate (n = 3). Results were analyzed by one-way ANOVA, followed by the Dunnett's test posttest). *: significant difference (* P<0.05; ** P<0.01), ns: no significant difference.
From: Nguyen TMD, Klett D, Filliatreau L, Combarnous Y (2019)
Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation.
PLoS ONE 14(6): e0217519.
doi: 10.1371/journal.pone.0217519
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Progesterone
- Product Type
- Monoclonal Antibody
- Clone
- 9B4
- Isotype
- IgG1
- Specificity
- Progesterone
Antibody Quality and Performance Guarantee
| Mouse anti progesterone antibody, clone 9B4 is specific for progesterone, a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage. Mouse anti progesterone antibody, clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone. |
- Target Species
- Broad
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Progesterone conjugated to bovine serum albumin at position 3.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Paraffin 1 | |
1/50 |
- 1 Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast carcinoma and normal young ovarian tissue.
References for Progesterone antibody
-
Cahill, D.J. et al. (2003) Pre-ovulatory granulosa cells of infertile women with endometriosis are less sensitive to luteinizing hormone.
Am J Reprod Immunol. 49 (2): 66-9. -
Alexandre-Pires, G. et al. (2014) Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes.
Microsc Res Tech. 77 (4): 313-25. -
Daems, D. et al. (2017) Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor.
Anal Chim Acta. 950: 1-6. -
Nguyen, T.M.D. et al. (2019) Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation.
PLoS One. 14 (6): e0217519. -
Fanelli, D. et al. (2019) Studies on the Use of Prostaglandin F2α and Gonadotropin-Releasing Hormone Analogs for Timed Artificial Insemination in Jennies
J Equine Vet Sci. 74: 36-41.
- RRID
- AB_620620
7720-1704
152440
161158
170202
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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