Progesterone antibody | 9B4
Mouse anti progesterone antibody, clone 9B4 (7720-1704) used to evaluate progesterone production by MLTC-1, Leydig tumor cells by ELISA.
Image caption:
Effect of fluoxetine in hLH or FSK/sub-hLH -promoted steroid production in MLTC-1 cells.
(A), (B) MLTC-1 cells were preincubated with or without FLX (or MET, or A-769662) for 1 hour and then the cells were stimulated for 3 hours with hLH or FSK/sub-hLH before progesterone productions were measured. Data are means ± SEM of 3 independent experiments performed in duplicate (n = 3). Results were analyzed by one-way ANOVA, followed by the Dunnett's test posttest). *: significant difference (* P<0.05; ** P<0.01), ns: no significant difference.
From: Nguyen TMD, Klett D, Filliatreau L, Combarnous Y (2019)
Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation.
PLoS ONE 14(6): e0217519.
doi: 10.1371/journal.pone.0217519
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti progesterone antibody, clone 9B4 is specific for progesterone, a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland.
Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.
Mouse anti progesterone antibody, clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone.
Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.
Mouse anti progesterone antibody, clone 9B4 does not cross react with cortisol, estradiol, estriol and testosterone.
Product Details
- Target Species
- Broad
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Progesterone conjugated to bovine serum albumin at position 3.
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Progesterone antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | 1/50 |
- 1 Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast carcinoma and normal young ovarian tissue.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Progesterone antibody
-
Cahill, D.J. et al. (2003) Pre-ovulatory granulosa cells of infertile women with endometriosis are less sensitive to luteinizing hormone.
Am J Reprod Immunol. 49 (2): 66-9. -
Alexandre-Pires, G. et al. (2014) Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes.
Microsc Res Tech. 77 (4): 313-25. -
Daems, D. et al. (2017) Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor.
Anal Chim Acta. 950: 1-6. -
Nguyen, T.M.D. et al. (2019) Inhibition by fluoxetine of LH-stimulated cyclic AMP synthesis in tumor Leydig cells partly involves AMPK activation.
PLoS One. 14 (6): e0217519.
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