Surfactant Protein D antibody | 1.7
Mouse anti Porcine surfactant protein D antibody, clone 1.7 (MCA2725) used for the evaluation of Surfactant protein D expression on porcine lung tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Lung injury after AH.
D) Results of IHC staining of surfactant protein D in lung tissue.
E) representative images of surfactant D staining. * p = 0.05, n.s. = not significant, graphical representation as mean ± SEM, NaCl n = 6, RBC n = 9, sham n = 6.
From: Weber B, Mendler MR, Lackner I, von Zelewski A, Höfler S, Baur M, et al. (2019)
Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.
PLoS ONE 14(7): e0219211.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig Surfactant Protein D
- Product Type
- Monoclonal Antibody
- Clone
- 1.7
- Isotype
- IgG1
- Specificity
- Surfactant Protein D
Antibody Quality and Performance Guarantee
| Mouse anti Porcine surfactant protein D antibody, clone 1.7 detects porcine surfactant protein D (pSP-D), a ~138 kDa member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. pSP-D may serve as a serum biomarker specific for bronchopneumonia in pigs (Kahlisch et al. 2009). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified porcine surfactant protein D.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of theP3X63Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
1µg/ml | |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
TRIS/EDTA buffer pH8.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Porcine lung tissue
- Western Blotting
- MCA2725 detects a band of approximately 138kDa.
Source Reference
-
Soerensen, C.M. et al. (2005) Purification, characterization and immunolocalization of porcine surfactant protein D.
Immunology. 114: 72-82.
References for Surfactant Protein D antibody
-
Soerensen, C.M. et al. (2005) Pulmonary infections in swine induce altered porcine surfactant protein D expression and localization to dendritic cells in bronchial-associated lymphoid tissue.
Immunology. 115 (4): 526-35. -
Weber, B. et al. (2019) Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.
PLoS One. 14 (7): e0219211.
- Synonyms
- SP-D
- RRID
- AB_10544550
- UniProt
- Q9N1X4
- Entrez Gene
- SFTPD
- GO Terms
- GO:0005529 sugar binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0007585 respiratory gaseous exchange
- GO:0043129 surfactant homeostasis
- GO:0048286 lung alveolus development
MCA2725
1511
158687
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