Surfactant Protein D antibody | 1.7

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Anti Pig Surfactant Protein D Antibody, clone 1.7 gallery image 1 — Staining of frozen pig lung with Mouse anti pig Surfactant Protein D (**MCA2725**).

Mouse anti Pig Surfactant Protein D

Product Type: Monoclonal Antibody
Clone: 1.7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2725	C IF P* WB			0.25 mg

Mouse anti Porcine surfactant protein D antibody, clone 1.7 detects porcine surfactant protein D (pSP-D), a ~138 kDa member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs.

Product Details

Target Species: Pig
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Purified porcine surfactant protein D.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of theP3X63Ag.8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q9N1X4 Related reagents
Entrez Gene: SFTPD Related reagents
GO Terms: GO:0005529 sugar binding; GO:0005578 proteinaceous extracellular matrix; GO:0005615 extracellular space; GO:0007585 respiratory gaseous exchange; GO:0043129 surfactant homeostasis; GO:0048286 lung alveolus development
Regulatory: For research purposes only

Applications of Surfactant Protein D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Immunohistology - Frozen		1µg/ml
Immunohistology - Paraffin ¹
Western Blotting

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

TRIS/EDTA buffer pH8.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Porcine lung tissue
Western Blotting: MCA2725 detects a band of approximately 138kDa.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Surfactant Protein D antibody

Soerensen, C.M. et al. (2005) Purification, characterization and immunolocalization of porcine surfactant protein D.
Immunology. 114: 72-82.
Soerensen, C.M. et al. (2005) Pulmonary infections in swine induce altered porcine surfactant protein D expression and localization to dendritic cells in bronchial-associated lymphoid tissue.
Immunology. 115 (4): 526-35.

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