SIGLEC-5 antibody | 4F7
Mouse anti Pig SIGLEC-5
- Product Type
- Monoclonal Antibody
- Clone
- 4F7
- Isotype
- IgG1
- Specificity
- SIGLEC-5
Antibody Quality and Performance Guarantee
| Mouse anti Pig siglec-5, clone 4F7 recognizes siglec-5 also known as CD170. Sialic-acid-binding immunoglobulin-like lectin (siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses. Siglec-5 is expressed on myeloid cells, neutrophils, monocytes, macrophages, blood eosinophils, and at low levels on plasmacytoid dendric cells. Mouse anti Pig Siglec-5 antibody, clone 4F7 has been successfully used to analyze siglec-5 expression in bone marrow and blood cells, and lymphoid tissues. Siglec-5 was found to be expressed on monocytes (CD172a+, CD16hi), with weak expression on B cells (CD79a+) and plasmacytoid DCs (PDCs). No expression was found on T cells or NK cells (Escalona et al 2013). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein consisting of the extracellular portion of porcine Siglec-5 fused to the Fc portion of human IgG1 (pSiglec- 5-Fc)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mouse were fused with cells of SP2/0 murine plasmacytoma
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Escalona, Z. et al. (2014) Molecular characterization and expression of porcine Siglec-5.
Dev Comp Immunol. 44 (1): 206-16.
References for SIGLEC-5 antibody
-
Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
Comp Immunol Microbiol Infect Dis. 64: 31-39. -
Poderoso, T. et al. (2020) Expression of Siglec-1, -3, -5 and -10 in porcine cDC1 and cDC2 subsets from blood, spleen and lymph nodes and functional capabilities of these cells.
Dev Comp Immunol. 109: 103692. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells.12 (16): 2103.
- UniProt
- F1RP86
MCA6168GA
163335
165414
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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