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Siglec-10 antibody | 2E9

Mouse anti Pig Siglec-10

Product Type
Monoclonal Antibody
Clone
2E9
Isotype
IgG1
Specificity
Siglec-10

Product Code Applications Pack Size List Price Your Price Qty
MCA6169GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig Siglec-10, clone 2E9 recognizes Siglec-10.

Sialic-acid-binding immunoglobulin-like lectin (Siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses. When expressed on transfected cells, porcine Siglec-10 is able to bind red blood cells in a sialic acid-dependent manner.

Mouse anti Pig Siglec-10 antibody, clone 2E9 has been successfully used to examine Siglec-10 cell and tissue distribution in swine. Siglec-10 was found to be expressed on blood B cells (CD21+ or CD79a+) and in B cell areas of the spleen and lymph nodes. Weak expression was also detected on monocytes (Escalona et al. 2014).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
NIH/3T3 mouse cells transfected with plasmid pSiglec-10-GFP, encoding the porcine Siglec-10 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Source Reference

  1. Escalona, Z. et al. (2015) Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues.
    Dev Comp Immunol. 48 (1): 116-23.

References for Siglec-10 antibody

  1. Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
    Comp Immunol Microbiol Infect Dis. 64: 31-39.
  2. Poderoso, T. et al. (2019) Analysis of the expression of porcine CD200R1 and CD200R1L by using newly developed monoclonal antibodies.
    Dev Comp Immunol. 100: 103417.
  3. Poderoso, T. et al. (2020) Expression of Siglec-1, -3, -5 and -10 in porcine cDC1 and cDC2 subsets from blood, spleen and lymph nodes and functional capabilities of these cells.
    Dev Comp Immunol. 109: 103692.
  4. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16): 2103.

MCA6169GA

163340 164932

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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