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Monocyte/Granulocyte antibody | 74-22-15

Mouse anti Pig Monocyte/Granulocyte

Product Type
Monoclonal Antibody
Clone
74-22-15
Isotype
IgG1
Specificity
Monocyte/Granulocyte

Product Code Applications Pack Size List Price Your Price Qty
MCA6100GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15, recognizes 90% of pig monocytes and granulocytes, in addition to 5% of lymphocytes, in peripheral blood. The target of Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15 is CD172a, similarly to clone BL1H7 (Haverson et al. 1994, Blecha et al. 1994). CD172a/SWC3a is expressed on monocytes, dendritic cells, and granulocytes.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Fresh dd miniature swine thymocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

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Mouse anti Pig CD14 MCA1218GA C F IF 0.1 mg loader
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Description Mouse anti Pig CD14

References for Monocyte/Granulocyte antibody

  1. Labarque, G.G. et al. (2000) Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs.
    J Gen Virol. 81 (Pt 5): 1327-34.
  2. Blecha, F. et al. (1994) Workshop studies on monoclonal antibodies reactive against porcine myeloid cells.
    Vet Immunol Immunopathol. 43 (1-3): 269-72.
  3. Jarosz, ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
    Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].

Synonyms
CD172a
SWC3a

MCA6100GA

153241 157184 160495 164243

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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