Monocyte/Granulocyte antibody | 74-22-15

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Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 thumbnail image 1

Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 thumbnail image 2

Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 gallery image 1 — Figure A. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and RPE conjugated Mouse anti Pig Monocyte/Granulocyte (**MCA6100PE**). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Pig Monocyte/Granulocyte Antibody, clone 74-22-15 gallery image 2 — Figure A. FITC conjugated Mouse anti Pig Granulocytes (MCA2600F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Pig Granulocytes (MCA2600F) and RPE conjugated Mouse anti Pig Monocyte/Granulocyte (**MCA6100PE**). All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Pig Monocyte/Granulocyte:FITC

Mouse anti Pig Monocyte/Granulocyte

Mouse anti Pig Monocyte/Granulocyte:RPE

Product Type: Monoclonal Antibody
Clone: 74-22-15
Isotype: IgG1

Product Code	Applications	Pack Size
MCA6100F	F	0.1 mg
MCA6100GA	F	0.1 mg
MCA6100PE	F	0.1 mg

Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15, recognizes 90% of pig monocytes and granulocytes, in addition to 5% of lymphocytes, in peripheral blood. Studies have reported that clone 74-22-15 recognizes CD172a, similarly to clone BL1H7 (Haverson et al. 1994). CD172a/SWC3a is expressed on monocytes, dendritic cells, and granulocytes.

Product Details

Target Species: Pig
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation: Purified IgG prepared by combination of precipitation and chromatography techniques
Preparation: Purified IgG prepared by combination of precipitation and chromatography techniques
Buffer Solution: Phosphate buffered saline
Buffer Solution: Borate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.1% Sodium Azide (NaN₃)
<10% Sucrose
Immunogen: Fresh dd miniature swine thymocytes
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of despatch
Shelf Life: Please see the label for the expiry

More Information

Regulatory: For research purposes only

Applications of Monocyte/Granulocyte antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry	Neat
Flow Cytometry	Neat	1/5

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse anti Pig CD14	MCA1218GA	0.1 mg	C F IF
Mouse anti Pig CD14:FITC	MCA1218F	0.1 mg	F

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