Monocyte/Granulocyte antibody | 74-22-15
Mouse anti Pig Monocyte/Granulocyte:FITC
Mouse anti Pig Monocyte/Granulocyte
Mouse anti Pig Monocyte/Granulocyte:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 74-22-15
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6100F | F | 0.1 mg |
|
|
| MCA6100GA | F | 0.1 mg | ||
| MCA6100PE | F | 0.1 mg |
|
Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15, recognizes 90% of pig monocytes and granulocytes, in addition to 5% of lymphocytes, in peripheral blood. Subsequent studies have reported that Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15 recognizes CD172a, similarly to clone BL1H7 (Haverson et al. 1994, Blecha et al. 1994). CD172a/SWC3a is expressed on monocytes, dendritic cells, and granulocytes.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Fresh dd miniature swine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of Monocyte/Granulocyte antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD14 | MCA1218GA | C F IF | 0.1 mg |
|
|
| Mouse anti Pig CD14:FITC | MCA1218F | F | 0.1 mg |
|
Product Specific References
References for Monocyte/Granulocyte antibody
-
Labarque, G.G. et al. (2000) Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs.
J Gen Virol. 81 (Pt 5): 1327-34. -
Blecha, F. et al. (1994) Workshop studies on monoclonal antibodies reactive against porcine myeloid cells.
Vet Immunol Immunopathol. 43 (1-3): 269-72. -
Jarosz, ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].
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